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131.
The evolutionary dynamics of the lion Panthera leo revealed by host and viral population genomics 下载免费PDF全文
Antunes A Troyer JL Roelke ME Pecon-Slattery J Packer C Winterbach C Winterbach H Hemson G Frank L Stander P Siefert L Driciru M Funston PJ Alexander KA Prager KC Mills G Wildt D Bush M O'Brien SJ Johnson WE 《PLoS genetics》2008,4(11):e1000251
The lion Panthera leo is one of the world's most charismatic carnivores and is one of Africa's key predators. Here, we used a large dataset from 357 lions comprehending 1.13 megabases of sequence data and genotypes from 22 microsatellite loci to characterize its recent evolutionary history. Patterns of molecular genetic variation in multiple maternal (mtDNA), paternal (Y-chromosome), and biparental nuclear (nDNA) genetic markers were compared with patterns of sequence and subtype variation of the lion feline immunodeficiency virus (FIV(Ple)), a lentivirus analogous to human immunodeficiency virus (HIV). In spite of the ability of lions to disperse long distances, patterns of lion genetic diversity suggest substantial population subdivision (mtDNA Phi(ST) = 0.92; nDNA F(ST) = 0.18), and reduced gene flow, which, along with large differences in sero-prevalence of six distinct FIV(Ple) subtypes among lion populations, refute the hypothesis that African lions consist of a single panmictic population. Our results suggest that extant lion populations derive from several Pleistocene refugia in East and Southern Africa ( approximately 324,000-169,000 years ago), which expanded during the Late Pleistocene ( approximately 100,000 years ago) into Central and North Africa and into Asia. During the Pleistocene/Holocene transition ( approximately 14,000-7,000 years), another expansion occurred from southern refugia northwards towards East Africa, causing population interbreeding. In particular, lion and FIV(Ple) variation affirms that the large, well-studied lion population occupying the greater Serengeti Ecosystem is derived from three distinct populations that admixed recently. 相似文献
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134.
Background
Demographic models are widely used in conservation and management, and their parameterisation often relies on data collected for other purposes. When underlying data lack clear indications of associated uncertainty, modellers often fail to account for that uncertainty in model outputs, such as estimates of population growth.Methodology/Principal Findings
We applied a likelihood approach to infer uncertainty retrospectively from point estimates of vital rates. Combining this with resampling techniques and projection modelling, we show that confidence intervals for population growth estimates are easy to derive. We used similar techniques to examine the effects of sample size on uncertainty. Our approach is illustrated using data on the red fox, Vulpes vulpes, a predator of ecological and cultural importance, and the most widespread extant terrestrial mammal. We show that uncertainty surrounding estimated population growth rates can be high, even for relatively well-studied populations. Halving that uncertainty typically requires a quadrupling of sampling effort.Conclusions/Significance
Our results compel caution when comparing demographic trends between populations without accounting for uncertainty. Our methods will be widely applicable to demographic studies of many species. 相似文献135.
Abstract. Plant defense theories suggest that chemical or structural defences should be maximized when and where browsing is most likely to occur. We tested this hypothesis on four evergreen woody species growing in a Mediterranean area with a high density of ungulates. In this system, levels of browsing are more intense in the winter (due to the lack of annual plants) and young foliage is often preferred. Therefore we predicted that the chemical defences of these species, namely their phenolic content, would vary with leaf age, season and damage intensity. In addition, we tested whether ungulates preferentially selected species containing lower phenolic levels, and also whether browsing induced either chemical or morphological changes in damaged plants. Phenolic levels varied greatly between plant species; ungulates browsed preferentially on the species with the lowest phenolic levels. No difference in phenolic content was found between browsed and unbrowsed trees. Morphological changes in heavily browsed trees included an increase in shoot and leaf density and a net decrease in leaf size. We suggest that for Mediterranean plants, which have evolved under high browsing pressure from large mammals, the production of small leaves and dense shoots in response to browsing might decrease ungulate foraging efficiency and hence reduce the rate of further damage as effectively as high levels of chemical defence. 相似文献
136.
Bengt Steen 《The International Journal of Life Cycle Assessment》2007,12(2):103-108
Goal, Scope and Background The life cycles of many products including textiles contain chemicals for which process flow data are not known or are too
time consuming to collect. Although each chemical may not contribute significantly to the LCA results of the product, which
might justify excluding them, but together their contribution could be significant. Similarly, rough estimates of the process
flows for the production of a single chemical may be very uncertain and considered meaningless, while the estimates of the
cumulative data of process flows for several chemicals may be less uncertain and be a meaningful contribution to the quality
of the LCA results. There are methods for estimation of process flows for different types of products, with varying demands
regarding input data and time and with varying accuracy of the results. This work contributes to the available methods, focusing
on simple estimations for production of chemical substances. The goal was to create a fast method for estimation of emissions,
resource and energy flows (process flows) for the production of chemicals, based on easily available data on the properties
of the chemicals. The process flows investigated were limited to those normally associated with process industries and contributing
most to depletion of resources, to global warming, acidification, eutrophication and photochemical ozone production, i.e.
use of energy, crude oil, coal, natural gas, uranium in ore and emissions of CO2, SOx, NOx, NMVOC, methane, BOD, COD and total
N. Toxic substances were excluded, since toxic emissions are substance specific and cannot be included in a generalization.
Method Available data for the process flows for the production of chemicals of mainly fossil origin were correlated to properties
of chemicals such as amount of carbon in the molecule, heat of formation and average number of chemical reaction steps in
the production. The production procedures were found in readily available literature. Up to about six reaction steps were
evaluated in the correlation study. The variations in the process flows among the chemicals studied were calculated.
Results and Discussion There were weak correlations between average number of chemical reaction steps in the production and energy use, COD measured
in water emissions, and SOx and NOx emissions to air. For the remaining properties of chemicals and process flows, there were
only weak correlations for share of double bonding in the molecule if only molecules containing double bondings were included.
Conclusions The precision in estimation of the process flows increases non-significantly when adding information on the number of reaction
steps or share of double bonding for chemicals containing double bonding is added.
Recommendations and Outlook It seems reasonable to start with a simple grouping method to estimate the process flows for the production of a chemical
of fossil origin. Further investigations might investigate whether there is a correlation between process flows and the costs
of chemicals, and further study the correlations between process flows and share of double bonding for chemicals containing
double bondings. 相似文献
137.
Images of human erythrocytes from a healthy donor were recorded under differential interference contrast (DIC) microscopy;
they were acquired rapidly (~336 Hz) and the intensity of the centermost pixel of each cell was recorded for ~60 s (20,000
values). Various techniques were used to analyze the data, including detrended fluctuation analysis (DFA) and multiscale entropy
(MSE); however, power spectrum analysis was deemed the most appropriate for metrifying and comparing results. This analysis
was used to compare cells from young and old populations, and after perturbing normal conditions, with changes in temperature,
adenosine triphosphate (ATP) concentration (using NaF, an inhibitor of glycolysis, and α-toxin, a pore-forming molecule used
to permeabilize red cells to ATP), and water transport rates [using glycerol, and p-chloromercuriphenylsulfonic acid (pCMBS) to inhibit aquaporins, AQPs]. There were measurable differences in the membrane
fluctuation characteristics in populations of young and old cells, but there was no significant change in the flickering time
series on changing the temperature of an individual cell, by depleting it of ATP, or by competing with the minor water exchange
pathway via AQP3 using glycerol. However, pCMBS, which inhibits AQP1, the major water exchange pathway, inhibited flickering
in all cells, and yet it was restored by the membrane intercalating species dibutyl phthalate (DBP). We developed a computer
model to simulate acquired displacement spectral time courses and to evaluate various methods of data analysis, and showed
how the flexibility of the membrane, as defined in the model, affects the flickering time course. 相似文献
138.
Charles G. Crispens Philip S. Porter John R. J. Sorenson 《Biological trace element research》1986,10(1):19-24
Plasma copper and zinc levels were measured in SJL/J mice, an inbred strain characterized by a high, spontaneous incidence
of reticulum cell sarcoma (RCS). The changes with age in mean concentrations of these metals were consistent with a physiological
response that is required for remission of neoplasia. Treatment of SJL/J mice with a copper complex, Cu(II)(3,4-diisopropylsalicylate)2 (Cu 3,5-DIPS), dissolved in a 10% Tween 80-saline solution revealed a decrease in survival and decline in the incidence of
RCS at 52 wk of age. The toxic effects of Cu 3,5-DIPS therapy appeared to be related to the intraperitoneal route of administration
and to extracellular deposition of collagen. The inhibitory effect on tumor development was not related to Cu 3,5-DIPS. Rather,
Tween 80 was found to be the factor of importance. 相似文献
139.
Extraction of Dictyonema glabratum with hot 2% (w/v) aqueous KOH at 100 degrees C, followed by neutralisation and freeze-thawing, gave an insoluble glucan. The residue was further extracted by a similar process, but with hot 10% (w/v) aqueous KOH, furnishing a mixture of glucan, mannan and xylan. The mannan and xylan were obtained via precipitation of its copper complex with Fehling's solution, leaving the glucan in the supernatant. The insoluble complex was finally purified through gel permeation chromatography. Methylation analysis, one- and two-dimensional nuclear magnetic resonance examination showed the polysaccharides to be a (1-->3)-linked alpha-glucan (pseudonigeran) and a (1-->4)-linked beta-xylan, both not previously encountered in lichens, and a newly discovered (1-->6)-linked beta-mannan. 相似文献
140.
Structures of the O-linked oligosaccharides of a complex glycoconjugate from Pseudallescheria boydii
Nonreducing O-linked oligosaccharides were obtained from the peptidorhamnomannan of mycelia of Pseudallescheria boydii by alkaline beta-elimination under reducing conditions. They were separated by gel filtration chromatography to give three oligosaccharide fractions. The major oligosaccharide from fraction 1 was characterized by a combination of techniques including electrospray ionization quadrupole time-of-flight tandem mass spectrometry (ESI MS/MS), matrix-assisted laser desorption ionization mass spectrometry (MALDI MS), nuclear magnetic resonance (NMR), and methylation gas-liquid chromatography-mass spectrometry (GC-MS) analysis. It was branched, with a principal chain of alpha-Rhap-(1 --> 3)-alpha-Rhap-(1 --> 3)-alpha-Manp-(1 --> 2)-Man-ol substituted at O-6 of mannitol with an alpha-Glcp-(1 --> 4)-beta-Galp group. Species containing one and two additional alpha-Glcp-(1 --> 4) substituents in the rhamnose branch were also present. The major component of fraction 2 was a substructure of oligosaccharide-1, lacking a hexose from the Glc-Gal branch. Fraction 3 contained a mixture of smaller, unbranched, oligosaccharides. In hapten inhibition tests, fractions 1 and 2 blocked the reaction between peptidorhamnomannan (PRM) and rabbit anti-P. boydii mycelium hyperimmune serum by approximately 75%, whereas fraction 3 inhibited by approximately 55%. 相似文献