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Liu Xiaoxiao Zhang Li Thu Pyone Myat Min Wenjian Yang Peng Li Ji Li Ping Xu Xiaojun 《中国科学:生命科学英文版》2021,64(8):1295-1310
Science China Life Sciences - Despite the use of many types of chemotherapies for pancreatic cancer, optimal efficacy has not been obtained so far. Pancreatic cancer shows a high incidence of TP53... 相似文献
74.
Van T. Hoang Quynh-Mai Trinh Dam Thi Minh Phuong Hue Thi Hong Bui Le Minh Hang Nguyen Thi Hong Ngan Nguyen Thi Tuyet Anh Phung Yen Nhi Trinh Thi Hong Nhung Ha Thi Lien Tu Dac Nguyen Liem Nguyen Thanh Duc M. Hoang 《Cytotherapy》2021,23(1):88-99
Background aimsMesenchymal stem/stromal cells (MSCs) are of interest for the treatment of graft-versus-host disease, autoimmune diseases, osteoarthritis and neurological and cardiovascular diseases. Increasing numbers of clinical trials emphasize the need for standardized manufacturing of these cells. However, many challenges related to diverse isolation and expansion protocols and differences in cell tissue sources exist. As a result, the cell products used in numerous trials vary greatly in characteristics and potency.MethodsThe authors have established a standardized culture platform using xeno- and serum-free commercial media for expansion of MSCs derived from umbilical cord (UC), bone marrow and adipose-derived (AD) and examined their functional characteristics.ResultsMSCs from the tested sources stably expanded in vitro and retained their biomarker expression and normal karyotype at early and later passages and after cryopreservation. MSCs were capable of colony formation and successfully differentiated into osteogenic, adipogenic and chondrogenic lineages. Pilot expansion of UC-MSCs and AD-MSCs to clinical scale revealed that the cells met the required quality standard for therapeutic applications.ConclusionsThe authors’ data suggest that xeno- and serum-free culture conditions are suitable for large-scale expansion and enable comparative study of MSCs of different origins. This is of importance for therapeutic purposes, especially because of the numerous variations in pre-clinical and clinical protocols for MSC-based products. 相似文献
75.
Nguyen Thi Quynh Mai Nguyen Thi Kim Thu Le Dac Tuyen Truong Chi Lam Vu Dinh Lam Nguyen Thi Quynh Hoa 《Plasmonics (Norwell, Mass.)》2021,16(5):1583-1592
Plasmonics - Developing a simple structure using low-cost material that enables both large-scale fabrication and broadband absorption response is highly desirable but very challenging for achieving... 相似文献
76.
Takuji Tachi Yu-Zen Huang Shin Komagata Kunio Araya Mahadimenakbar Mohamed Dawood Thai Hong Pham Ding Yang Theo Zeegers Hans-Peter Tschorsnig Hiroshi Shima 《Journal of Asia》2021,24(1):285-296
Compsilura concinnata (Meigen) is one of the most famous, most polyphagous and most widely distributed tachinid flies (Diptera, Tachinidae) in the world. This species is well known as a biocontrol agent of some injurious pests of cultural and wild plants and has been introduced from Europe to the United States to control mainly the gypsy moth. Recently we found three new species very closely resembling C. concinnata from Southeast and East Asia: C. lobata sp. nov. (Japan and Thailand), C. malayana sp. nov. (Malaysia) and C. pauciseta sp. nov. (Japan and Taiwan). Additionally, C. samoaensis Malloch is treated as a junior synonym of C. concinnata based on the examination of the type specimen. The genetic differences in the mitochondrial COI gene data are examined to assess the accuracy of species delimitation of Compsilura. The male postabdominal characters of these species are illustrated. The piercing female postabdomen of C. concinnata is illustrated and compared to those of other members belonging to the Blondelia group including Blondelia Robineau-Desvoidy, Celatoria Coquillett, Eucelatoria Townsend and Vibrissina Rondani. 相似文献
77.
William B. Parker Sue C. Shaddix Lucy M. Rose Phuong T. Pham Mei Hua Robert Vince 《Nucleosides, nucleotides & nucleic acids》2013,32(4):795-804
Abstract The metabolism of O6-propyl-carbovir and N6-propyl-carbovir, two selective inhibitors of HIV replication, has been evaluated in CEM cells. Both compounds were phosphorylated in intact cells to carbovir-5′-triphosphate. The metabolism of these two agents was inhibited by deoxycoformycin and mycophenolic acid, but not erythro-9-(2-hydroxy-3-nonyl)adenine. No evidence of the 5′-triphosphate of either compound was detected in CEM cells. 相似文献
78.
Karine Monceau Mariangela Arca Lisa Leprêtre Florence Mougel Olivier Bonnard Jean-Fran?ois Silvain Nevile Maher Gérard Arnold Denis Thiéry 《PloS one》2013,8(6)
Contrary to native predators, which have co-evolved with their prey, alien predators often benefit from native prey naïveté. Vespa velutina, a honeybee predator originating from Eastern China, was introduced into France just before 2004. The present study, based on video recordings of two beehives at an early stage of the invasion process, intends to analyse the alien hornet hunting behaviour on the native prey, Apis mellifera, and to understand the interaction between the activity of the predator and the prey during the day and the season. Chasing hornets spent most of their time hovering facing the hive, to catch flying honeybees returning to the hive. The predation pressure increased during the season confirming previous study based on predator trapping. The number of honeybee captures showed a maximum peak for an intermediate number of V. velutina, unrelated to honeybee activity, suggesting the occurrence of competition between hornets. The number of honeybees caught increased during midday hours while the number of hornets did not vary, suggesting an increase in their efficacy. These results suggest that the impact of V. velutina on honeybees is limited by its own biology and behaviour and did not match the pattern of activity of its prey. Also, it could have been advantageous during the invasion, limiting resource depletion and thus favouring colonisation. This lack of synchronization may also be beneficial for honeybee colonies by giving them an opportunity to increase their activity when the hornets are less effective. 相似文献
79.
Gerard J. Casey Antonio Montresor Luca T. Cavalli-Sforza Hoang Thu Luong B. Phu Ta T. Tinh Nong T. Tien Tran Q. Phuc Beverley-Ann Biggs 《PLoS neglected tropical diseases》2013,7(4)