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81.
Modification of Photosystem I Light Harvesting of Bundle-Sheath Chloroplasts Occurred during the Evolution of NADP-Malic Enzyme C4 Photosynthesis
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Low-temperature emission spectra and excitation spectra for chlorophyll fluorescence were recorded from leaves of species of the genus Flaveria (Asteraceae) with C3, C3-C4-intermediate, C4-like, and C4 photosynthesis. Among the latter two groups, high chlorophyll b absorption was observed in excitation spectra for photosystem I (PSI) fluorescence. By comparing leaf data with those from isolated chloroplast fractions, the high chlorophyll b absorption was attributed to the specific properties of the bundle-sheath chloroplasts in leaves from C4 plants. The deconvolution of the PSI excitation spectra and the use of a model revealed that the contribution of photosystem II absorption to the functional antenna of PSI was markedly increased in leaves from three of the five C4-like and C4 species investigated in detail. The two other species exhibited normal, C3-like light-harvesting properties of PSI. The former species are known for efficient carbon assimilation, the latter for decreased efficiencies of carbon assimilation. It is concluded that photosystem II becomes a substantial part of the functional PSI antenna late in the evolution of C4 photosynthesis, and that the composite antenna optimizes the light-harvesting of PSI in bundle-sheath chloroplasts to meet the energy requirements of C4 photosynthesis. 相似文献
82.
Chunjian Li Heidrun Pfeffer Frank Dannel Volker Römheld Fritz Bangerth 《Physiologia plantarum》2001,111(2):212-219
Two experiments were carried out to study the effects of boron (B) deficiency on 7-day-old pea plants for 6 or 9 days under controlled growth chamber conditions. Growth and apical dominance (AD) of the plants and their B concentration and compartmentation were followed throughout the starvation period. Additionally, auxin (indoleacetic acid, IAA) concentration in the shoot apex and polar transport from it were measured along with the cytokinin (CK) concentration in the shoot apex and the roots. The results demonstrate that during a 6-day B-deficiency period, B concentration in the water-insoluble residue of the roots was very stable and could not easily be reduced. In contrast, B concentration in the cell sap fraction was very sensitive to external B supply. Twelve hours after transferring the plants from B-sufficient to B-deficient solutions, the B concentration in root cell sap declined to half the concentration of the control plants. In addition, B concentration in the new aerial plant parts, which developed after the onset of the B-deficiency treatment, was extremely low. A decline in elongation growth could be observed as soon as about 4 days after the imposition of B deficiency. This preceded the first measurable growth of lateral buds (release from AD). Before the onset of these morphological changes, there was a considerable decline in CK concentration, accompanied by a dramatic decrease in IAA export out of the shoot apex, a decline in IAA concentration in the shoot apex and the roots and a reduced capacity for polar IAA-transport. These changes are discussed as possible reasons for the observed reduction in elongation growth and AD. These hormonal changes themselves are possibly the result of the decreased symplasmic B concentration, which in turn may be responsible for the reduced concentration in apical CKs. A sequence of events, which may be causally related, is suggested to explain the effects of B deficiency on the growth and AD of pea plants. 相似文献
83.
Replenishment of medium after 72 hr of growth of HeLa-S3 cells in dense suspension cultures increased [3H]-thymidine uptake into cells and incorporation into DNA, with the levels reaching a peak ~ 12 hr following medium change; β interferon inhibits the enhanced uptake of [3H]-thymidine and labeling of DNA in a dose-dependent manner. Some reduction in these processes is observed at a concentration as low as 1 u/ml, and ~ 75% inhibition at 640 u/ml. Kinetic analysis has revealed that the rate of labeling of the acid-soluble pool with [3H]-thymidine, measured either at 22°C, or 37°C, is reduced in interferon-treated (640 u/ml, 24 hr) HeLa-S3 cells. At 22°C, the initial rate of thymidine transport at a high (500 μM) thymidine concentration, determined within the first 30 sec of [3H]-thymidine addition was depressed by 44% in interferon-treated HeLa cells. At 37°C, labeled precursors accumulate in acid-soluble material for ~ 8 min after the addition of [3H]-thymidine, after which an apparent equilibrium level is attained. At this temperature, the rate of thymidine uptake and the apparent equilibrium level attained were depressed by 70% in interferon-treated HeLa cells. The reduced incorporation of [3H]-thymidine into DNA in interferon-treated HeLa-S3 cells can be largely explained by interferon inhibition of thymidine transport and phosphorylation. 相似文献
84.
Recruitment of TBK1 to cytosol‐invading Salmonella induces WIPI2‐dependent antibacterial autophagy
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Teresa LM Thurston Keith B Boyle Mark Allen Benjamin J Ravenhill Maryia Karpiyevich Stuart Bloor Annie Kaul Jessica Noad Agnes Foeglein Sophie A Matthews David Komander Mark Bycroft Felix Randow 《The EMBO journal》2016,35(16):1779-1792
Mammalian cells deploy autophagy to defend their cytosol against bacterial invaders. Anti‐bacterial autophagy relies on the core autophagy machinery, cargo receptors, and “eat‐me” signals such as galectin‐8 and ubiquitin that label bacteria as autophagy cargo. Anti‐bacterial autophagy also requires the kinase TBK1, whose role in autophagy has remained enigmatic. Here we show that recruitment of WIPI2, itself essential for anti‐bacterial autophagy, is dependent on the localization of catalytically active TBK1 to the vicinity of cytosolic bacteria. Experimental manipulation of TBK1 recruitment revealed that engagement of TBK1 with any of a variety of Salmonella‐associated “eat‐me” signals, including host‐derived glycans and K48‐ and K63‐linked ubiquitin chains, suffices to restrict bacterial proliferation. Promiscuity in recruiting TBK1 via independent signals may buffer TBK1 functionality from potential bacterial antagonism and thus be of evolutionary advantage to the host. 相似文献
85.
Carbon Uptake and the Metabolism and Transport of Lipids in an Arbuscular Mycorrhiza 总被引:15,自引:2,他引:13
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Philip E. Pfeffer David D. Douds Jr. Guillaume Bcard Yair Shachar-Hill 《Plant physiology》1999,120(2):587-598
Both the plant and the fungus benefit nutritionally in the arbuscular mycorrhizal symbiosis: The host plant enjoys enhanced mineral uptake and the fungus receives fixed carbon. In this exchange the uptake, metabolism, and translocation of carbon by the fungal partner are poorly understood. We therefore analyzed the fate of isotopically labeled substrates in an arbuscular mycorrhiza (in vitro cultures of Ri T-DNA-transformed carrot [Daucus carota] roots colonized by Glomus intraradices) using nuclear magnetic resonance spectroscopy. Labeling patterns observed in lipids and carbohydrates after substrates were supplied to the mycorrhizal roots or the extraradical mycelium indicated that: (a) 13C-labeled glucose and fructose (but not mannitol or succinate) are effectively taken up by the fungus within the root and are metabolized to yield labeled carbohydrates and lipids; (b) the extraradical mycelium does not use exogenous sugars for catabolism, storage, or transfer to the host; (c) the fungus converts sugars taken up in the root compartment into lipids that are then translocated to the extraradical mycelium (there being little or no lipid synthesis in the external mycelium); and (d) hexose in fungal tissue undergoes substantially higher fluxes through an oxidative pentose phosphate pathway than does hexose in the host plant. 相似文献
86.
Michael Wink Heidi Staudter Yevgeny Bragin Ralf Pfeffer Robert Kenward 《Journal of Ornithology》1999,140(4):481-489
Summary DNA fingerprinting of nestlings ofFalco cherrug was used to determine indirectly the survival of the corresponding adult parent birds, which are difficult to catch in sufficient numbers. This approach is possible because Saker falcons show a high degree of site and mate tenacity. DNA profiles of nestlings from the same territory but from different years were compared. Three patterns of band-sharing coefficients between broods from the same territory were found: if band-sharing coefficients within and between broods from consecutive years were similar but significantly different from those of unrelated birds, it indicated that all young were full sibs and that neither adult was replaced between years. If band-sharing coefficients between broods at the same site indicated no relatedness across years and were equal to those of unrelated birds, then both breeding partners apparently had changed. If the band-sharing coefficients between broods of the same territory and consecutive years were significantly lower than those of full sibs, but higher than those of unrelated birds, the loss of one adult bird was indicated. The analysis of 32 broods (years 1993 to 1997) provided a minimal estimate for annual adult survival of 82% for a wild population of Saker Falcons in Kazakhstan.
Abschätzung der jährlichen Überlebensraten des Sakerfalken(Falco cherrug) mittels DNA-Fingerprinting
Zusammenfassung Um die Gefährdung und Populationsdynamik des Sakerfalken (Falco cherrug) beurteilen zu können, benötigen wir genaue Angaben zu Mortalität und Überlebensraten. Während es bei dieser Art relativ einfach ist, Nestlinge zu fangen, ist es nahezu unmöglich, eine ausreichend große Zahl an Altvögeln zu markieren, um durch Wiederfang oder Ringfundmeldungen die jährliche Überlebensrate zu ermitteln. Durch DNA-Fingerprinting von Jungfalken haben wir versucht, die minimale Überlebensrate von Altfalken indirekt zu bestimmen. Dieser Forschungsansatz wird dadurch möglich, daß die Sakerfalken eine hohe Philopatrie aufweisen und jedes Jahr im selben Revier brüten. Wenn man mehrere Jahre lang Blutproben der Jungvögel aus denselben Revieren sammelt, so kann man mittels DNA Fingerprinting indirekt ermitteln, ob die jeweiligen Altvögel identisch waren oder gewechselt haben: Vergleicht man die Band-Sharing-Koeffizienten (BSK) von Jungvögeln von zwei oder mehr Jahren aus demselben Revier, so ergeben sich drei Muster: Wenn die BSK-Werte innerhalb der Bruten und zwischen den Bruten identisch aber signifikant verschieden von denen nicht verwandter Vögel sind, so handelt es sich bei den Jungvögeln um Vollgeschwister; demnach sind die Altvögel identisch geblieben, d. h. sie haben von einem Jahr zum nächsten überlebt. Wenn die BSK-Werte zwischen zwei Bruten aus demselben Revier einen Wert annehmen, wie man ihn für unverwandte Tiere ermittelt, so müssen die Eltern gewechselt haben. Liegen die Werte zwischen zwei Bruten signifikant höher als die von nicht verwandten Tieren, aber niedriger als diejenigen von Vollgeschwistern, so ist vermutlich 1 Altvogel gewechselt worden. Die Analyse von 32 Bruten des Sakerfalken aus Kasachstan zeigt, daß die minimale jährliche Adultüberlebensrate bei 82% liegt.相似文献
87.
88.
Scott Emr Benjamin S. Glick Adam D. Linstedt Jennifer Lippincott-Schwartz Alberto Luini Vivek Malhotra Brad J. Marsh Akihiko Nakano Suzanne R. Pfeffer Catherine Rabouille James E. Rothman Graham Warren Felix T. Wieland 《The Journal of cell biology》2009,187(4):449-453
The Golgi apparatus is essential for protein sorting and transport. Many researchers have long been fascinated with the form and function of this organelle. Yet, despite decades of scrutiny, the mechanisms by which proteins are transported across the Golgi remain controversial. At a recent meeting, many prominent Golgi researchers assembled to critically evaluate the core issues in the field. This report presents the outcome of their discussions and highlights the key open questions that will help guide the field into a new era. 相似文献
89.
90.
Emily HM Wong David K Smith Raul Rabadan Malik Peiris Leo LM Poon 《BMC evolutionary biology》2010,10(1):253