排序方式: 共有49条查询结果,搜索用时 15 毫秒
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K. K. Lal A. Mandal R. K. Singh P. Punia D. Kapoor U. K. Chauhan S. P. Singh V. Mohindra 《Zeitschrift fur angewandte Ichthyologie》2006,22(5):369-373
Allozyme and RAPD profiles reveal markers that discriminate Chitala chitala and Notopterus notopterus. Thirty‐five allozyme loci were scored from 23 allozyme systems. Species‐specific differences were found at 16 loci. Fifteen RAPD markers with 77 size fragments, 244–2902 bp, were identified. The number of fragments specific to C. chitala and N. notopterus was found to be 20 and 31, respectively. Theta estimates of 0.9798 (allozymes) and 0.9471 (RAPD) indicated a large genetic divergence between C. chitala and N. notopterus. The observed genetic heterogeneity clearly demonstrated that the two genera, Chitala and Notopterus, are distinct from each other. 相似文献
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Chaturvedi A Mohindra V Singh RK Lal KK Punia P Bhaskar R Mandal A Narain L Lakra WS 《Molecular biology reports》2011,38(5):3513-3529
We examined population structure of Labeo dero (Hamilton, 1822) from different riverine locations in India using 10 polymorphic allozyme and eight microsatellite loci.
For analysis, 591 different tissue samples were obtained from commercial catches covering a wide geographic range. Allozyme
variability (An = 1.28–1.43, Ho = 0.029–0.071) was much lower than for microsatellites (An = 4.625–6.125, Ho = 0.538–0.633).
Existence of rare alleles was found at three allozyme (MDH-2*, GPI* and PGDH*) and at two microsatellite loci (R-3* and MFW-15*). Deviation from Hardy–Weinberg equilibrium (P < 0.05, after the critical probability levels were adjusted for sequential Bonferroni adjustment) could be detected at three
loci (EST-1*, -2* and XDH*) whereas, after correction for null alleles, two microsatellite loci (MFW-1*,-15*) deviated from HWE in the river Yamuna. Fst for all the samples combined over all allozyme loci was found to be 0.059 suggesting
that 5.9% of the total variation was due to genetic differentiation while microsatellite analysis yielded 0.019 which was
concordant to mean Rst (0.02). Hierarchical partition of genetic diversity (AMOVA) showed that greater variability (approx.
95%) was due to within population component than between geographical regions. Based on distribution of genetic differentiation
detected by both markers, at least five different genetic stocks of L. dero across its natural distribution could be identified. These results are useful for the evaluation and conservation of L. dero in natural water bodies. 相似文献
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The randomly amplified polymorphic DNA (RAPD) markers were used to detect interspecific genetic variability and genetic relatedness
among five Indian sciaenids namely Otolithes cuvieri, Johnieops sina, Johnieops macrorhynus, Johnieops vogleri and Protonibea diacanthus for the first time. Eight RAPD primers (OPA01, OPA06, OPA07, OPA18, OPP12, OPP14, OPP16 and OPP11) generated 40 species specific
diagnostic bands. The highest genetic divergence was detected between J. macrorhynus and P. diacanthus (0.586) where as the lowest one was observed between J. sina and J. vogleri (0.274).
Handling editor: C. Strumbauer 相似文献
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KuldeepK.LAL RajeevK.SINGH VindhyaMOHINDRA AnupMANDAL PeyushPUNIA B.N.MISHRA W.S.LAKRA 《动物学报》2007,53(4):73-776
利用鉴别性的同工酶位点从印度恒河水系的支流Banganga中检测到了蓝黑鲮(Labeo calbasu)与南亚黑鲮(Labeorohita)的一个杂交个体。线粒体DNA的单倍型模式进一步确认这个杂交个体的母本是河蓝黑鲮。 相似文献
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Mandal A Mohindra V Singh RK Punia P Singh AK Lal KK 《Molecular biology reports》2012,39(2):1765-1775
Genetic variation at mitochondrial cytochrome b (cyt b) and D-loop region reveals the evidence of population sub-structuring in Indian populations of highly endangered primitive
feather-back fish Chitala chitala. Samples collected through commercial catches from eight riverine populations from different geographical locations of India
were analyzed for cyt b region (307 bp) and D-loop region (636–716 bp). The sequences of the both the mitochondrial regions revealed high haplotype
diversity and low nucleotide diversity. The patterns of genetic diversity, haplotypes networks clearly indicated two distinct
mitochondrial lineages and mismatch distribution strongly suggest a historical influence on the genetic structure of C. chitala populations. The baseline information on genetic variation and the evidence of population sub-structuring generated from
this study would be useful for planning effective strategies for conservation and rehabilitation of this highly endangered
species. 相似文献
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Luhariya RK Lal KK Singh RK Mohindra V Punia P Chauhan UK Gupta A Lakra WS 《Molecular biology reports》2012,39(4):3659-3665
The present study examined partial cytochrome b gene sequence of mitochondrial DNA for polymorphism and its suitability to determine the genetic differentiation in wild
Labeo rohita. The 146 samples of L. rohita were collected from nine distant rivers; Satluj, Brahmaputra, Son, Chambal Mahanadi, Rapti, Chauka, Bhagirathi and Tons were
analyzed. Sequencing of 307 bp of Cyto b gene revealed 35 haplotypes with haplotype diversity 0.751 and nucleotide diversity (π) 0.005. The within population variation
accounted for 84.21% of total variation and 15.79% was found to among population. The total Fst value, 0.158 (P < 0.05) was found to be significant. The results concluded that the partial cyto b is polymorphic and can be a potential marker to determining genetic stock structure of L. rohita wild population. 相似文献