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191.
Nuclear organization of splicing small nuclear ribonucleoproteins in adenovirus-infected cells. 总被引:10,自引:4,他引:6 下载免费PDF全文
We have studied the effect of adenovirus infection on the nuclear organization of splicing small nuclear ribonucleoproteins (snRNPs) in HeLa cells. In uninfected HeLa cells, snRNPs are widespread throughout the nucleoplasm but also are concentrated in specific nuclear structures, including coiled bodies, interchromatin granules, and perichromatin fibrils. We have used immunofluorescence microscopy to study the localization of splicing snRNPs relative to centers of viral DNA synthesis and accumulation identified with antiserum against the viral 72,000-molecular-weight single-stranded DNA-binding protein (72K protein). Splicing snRNPs were independently detected with both monoclonal and polyclonal antibodies specific for common snRNP antigens, snRNP-specific proteins, and the snRNA-specific 2,2,7-trimethylguanosine 5' cap structure. We have examined infected cells 2 to 24 h after infection, and, in the majority of these cells, we observed no colocalization of the snRNP and 72K-protein staining patterns. In the late phase, snRNPs were found to markedly concentrate in discrete clusters that were distinct from the centers of viral DNA synthesis and accumulation identified with anti-72K protein. We have treated cells with hydroxyurea at various times after infection to inhibit aspects of the virus infectious program. We have found that the accumulation of snRNP clusters is correlated with late gene expression rather than with DNA synthesis or early gene expression. Finally, we show that the late-phase snRNP clusters colocalize with a monoclonal antibody that primarily stains interchromatin granules. These results suggest that the centers of snRNP concentration in late-phase infected cells are likely to correspond to interchromatin granule clusters. 相似文献
192.
T. Lindberg T. A. Bonde L. Bergström R. Pettersson T. Rosswall J. Schnürer 《Plant and Soil》1989,119(1):25-37
An annual cereal, barley, and a perennial grass ley, meadow fescue, were grown in field lysimeters in Sweden and fertilized
with 12 and 20g Ca(NO3)2-N m−2 yr−1, respectively. Isotope-labeled (15N) fertilizer was added during year 1 of the study, whereafter similar amounts of unlabeled N were added during years 2 and
3. The grass ley lysimeters were ploughed after the growing season of year 3 and sown with barley during year 4. The barley
harvest in year 1 removed 59% of the added fertilizer N, while the fertilizer N export by two meadow fescue harvests in year
1 was 65%. The labeled N export decreased rapidly after year 1, especially in the barley, but increased slightly after ploughing
of the grass ley.
The microbial biomass, measured with the chloroform fumigation method, incorporated a maximum of 1.4–1.7% of the labeled N
during the first seven weeks after application. Later on, the incorporation stabilized at less than 1% in both cropping systems.
The susceptibility of the residual labeled N to mineralization was evaluated three years after application by means of long-term
laboratory incubations. The curves of cumulative mineralized N were described by a two-component first-order regression model
that differentiated between an available and a more recalcitrant fraction of potentially mineralizable N. There was no difference
in the amounts of potentially mineralizable N between the cropping systems. The labeled N comprised 5 and 2% of the amounts
of potentially mineralizable N in the available and more recalcitrant fraction, respectively. The mineralization rate constants
for the labeled N were almost twice as high as for the total potentially mineralizable N. The available fraction of the total
potentially mineralizable N was 12%, while twice that proportion of the labeled N was available.
It was concluded that the short-term ley did not differ from the annual crop with respect to the early disposition of the
fertilizer N and the behaviour of the residual organic N. 相似文献
193.
194.
195.
Growth and cellulase formation by Cellvibrio fulvus 总被引:1,自引:0,他引:1
196.
The electrical and mechanical activities of myocardial strips from Rana pipiens treated with isoproterenol (ISO) were studied during cyanide hypoxia at different bath temperatures (12, 25 and 35 degrees C). In normal myocardium at 12 degrees C, the action potential duration (APD) was almost unchanged but the isometric force (P) was reduced to about 60% after 30 min in 3 mmol/l NaCN. At 25 degrees C, APD and P decreased to about 80 and 60%, respectively, after exposure to cyanide for 30 min. At 35 degrees C, a fast decrease of APD (to about 30%) and P (to about 10%) was seen within 30 min. In all cases, washout of cyanide interactions was possible. Large effects occurred when ISO-damaged myocardium was exposed to cyanide. During the initial 30 min of CN-treatment, APD and P were significantly reduced in the whole temperature range between 12 and 35 degrees C, when compared with controls. When the cyanide exposure times were long enough, all preparations developed contracture. Increase of the temperature and/or ISO-pretreatment shortened the time-course for resting tension increase. The effects of cyanide on APD and resting tension (RT) were strongly correlated and presumably a result of a cyanide-induced rise of the intracellular free calcium concentration (Ca2+i). 相似文献
197.
198.
Vibeke S.M. Berson Per Lundberg Bertil Pettersson 《Biochimica et Biophysica Acta (BBA)/General Subjects》1979,587(3):353-361
Noradrenaline-stimulated oxidative metabolism in isolated hamster brown fat cells is very reproducible between different cell preparations, 565 ± 81 (S.D.) nmol O/min per 106 cells (n = 25).In contrast, the oxygen consumption rate induced by the addition of succinate or sn-glycerol 3-phosphate strongly varies between different cell preparation, although these substances have been reported to be potent substrates for isolated hamster brown fat cells.By filtration and by successive washings we demonstrate that the flavoprotein-linked substrate oxidation is mainly dependent on extracellular succinate and sn-glycerol 3-phosphate-oxidizing enzymes. These enzymes originate from damaged and broken cells and are present in different amounts in different cell preparations.In discriminating between intra- and extracellular succinate oxidation 5,5′-dithiobis(2-nitrobenzoate) is used as an inhibitor of the extracellular portion. This application of 5,5′-dithiobis(2-nitrobenzoate) ougth to be useful also in other cell or tissue preparations.Added succinate can, however, be oxidized by the intact brown adipocyte but a very low rate, probably as a result of a limited transport rate over the membrane(s). In the presence of noradrenaline, added succinated can potentiate the noradrenaline-inducible oxygen consumption by catalytically increasing the oxidative capacity of the citric acid cycle.Our conclusions is that the only effectors which significantly increase oxidative metabolism in intact isolated hamster brown fat cells are catecholamines and free fatty acids. Provided the cells are uncoupled, also pyruvate can function as substrate for these cells. 相似文献
199.
Caesar R Palmfeldt J Gustafsson JS Pettersson E Hashemi SH Blomberg A 《Proteomics》2007,7(22):4135-4147
The proteomes of three industrial lager beer strains, CMBS33, OG2252 and A15, were analysed under standardised laboratory growth conditions. Protein spots in the 2-DE pattern of the lager strains were subjected to MS/MS to identify protein variants. We found the protein composition of the three lager strains to be qualitatively rather similar, while being substantially different from the Saccharomyces cerevisiae strain BY4742. Database searches using several fully sequenced genomes from the Saccharomyces genera indicated that the non-cerevisiae proteins in the 2-D pattern of lager strains were most closely related to S. bayanus. For many proteins the regulation of the bayanus-like protein and its cerevisiae counterpart varied in a strain-dependent manner, e.g. the bayanus-like form of Tdh3p was roughly eight-fold more abundant than the cerevisiae form in the OG2252 strain. We also found differential regulation of cerevisiae- and bayanus-like proteins during various stress conditions like low temperature growth, and adaptation to high temperatures or high salinity, e.g. for Arg1p, Sti1p and Pdc1p. Our data on the differential regulation of the two genomes in these hybrid strains may have important industrial implications for strain improvement and strain protection. 相似文献
200.
Pettersson M Johnson DS Subramanyam C Bales KR am Ende CW Fish BA Green ME Kauffman GW Lira R Mullins PB Navaratnam T Sakya SM Stiff CM Tran TP Vetelino BC Xie L Zhang L Pustilnik LR Wood KM O'Donnell CJ 《Bioorganic & medicinal chemistry letters》2012,22(8):2906-2911
We report the discovery and optimization of a novel series of dihydrobenzofuran amides as γ-secretase modulators (GSMs). Strategies for aligning in vitro potency with drug-like physicochemical properties and good microsomal stability while avoiding P-gp mediated efflux are discussed. Lead compounds such as 35 and 43 have moderate to good in vitro potency and excellent selectivity against Notch. Good oral bioavailability was achieved as well as robust brain Aβ42 lowering activity at 100 mg/kg po dose. 相似文献