全文获取类型
收费全文 | 497篇 |
免费 | 29篇 |
出版年
2022年 | 4篇 |
2021年 | 8篇 |
2020年 | 5篇 |
2019年 | 4篇 |
2018年 | 9篇 |
2017年 | 11篇 |
2016年 | 8篇 |
2015年 | 23篇 |
2014年 | 27篇 |
2013年 | 33篇 |
2012年 | 37篇 |
2011年 | 27篇 |
2010年 | 28篇 |
2009年 | 21篇 |
2008年 | 27篇 |
2007年 | 27篇 |
2006年 | 18篇 |
2005年 | 21篇 |
2004年 | 13篇 |
2003年 | 17篇 |
2002年 | 9篇 |
2001年 | 4篇 |
2000年 | 2篇 |
1999年 | 3篇 |
1998年 | 6篇 |
1997年 | 5篇 |
1996年 | 4篇 |
1995年 | 9篇 |
1994年 | 7篇 |
1993年 | 7篇 |
1992年 | 3篇 |
1991年 | 9篇 |
1989年 | 3篇 |
1988年 | 9篇 |
1987年 | 7篇 |
1986年 | 4篇 |
1985年 | 4篇 |
1984年 | 5篇 |
1983年 | 4篇 |
1982年 | 5篇 |
1981年 | 3篇 |
1980年 | 5篇 |
1979年 | 2篇 |
1978年 | 4篇 |
1977年 | 3篇 |
1973年 | 2篇 |
1970年 | 2篇 |
1967年 | 2篇 |
1853年 | 2篇 |
1852年 | 17篇 |
排序方式: 共有526条查询结果,搜索用时 635 毫秒
51.
Carl Petter 《Plant Systematics and Evolution》1862,12(11):350-356
Ohne Zusammenfassung 相似文献
52.
53.
Per-Olof Eriksson Emil Aaltonen Rodrigo Petoral Jr Petter Lauritzson Hideki Miyazaki Kristian Pietras Sven M?nsson Lennart Hansson Peter Leander Oskar Axelsson 《PloS one》2014,9(10)
The current study was carried out to test the potential of a new nanomaterial (Spago Pix) as a macromolecular magnetic MR contrast agent for tumor detection and to verify the presence of nanomaterial in tumor tissue. Spago Pix, synthesized by Spago Nanomedical AB, is a nanomaterial with a globular shape, an average hydrodynamic diameter of 5 nm, and a relaxivity (r1) of approximately 30 (mM Mn)−1 s−1 (60 MHz). The material consists of an organophosphosilane hydrogel with strongly chelated manganese (II) ions and a covalently attached PEG surface layer. In vivo MRI of the MMTV-PyMT breast cancer model was performed on a 3 T clinical scanner. Tissues were thereafter analyzed for manganese and silicon content using inductively coupled plasma-atomic emission spectroscopy (ICP-AES). The presence of nanomaterial in tumor and muscle tissue was assessed using an anti-PEG monoclonal antibody. MR imaging of tumor-bearing mice (n = 7) showed a contrast enhancement factor of 1.8 (tumor versus muscle) at 30 minutes post-administration. Contrast was retained and further increased 2–4 hours after administration. ICP-AES and immunohistochemistry confirmed selective accumulation of nanomaterial in tumor tissue. A blood pharmacokinetics analysis showed that the concentration of Spago Pix gradually decreased over the first hour, which was in good agreement with the time frame in which the accumulation in tumor occurred. In summary, we demonstrate that Spago Pix selectively enhances MR tumor contrast in a clinically relevant animal model. Based on the generally higher vascular leakiness in malignant compared to benign tissue lesions, Spago Pix has the potential to significantly improve cancer diagnosis and characterization by MRI. 相似文献
54.
The dynamic nature of human working memory, the general-purpose system for processing continuous input, while keeping no longer externally available information active in the background, is well captured in immediate free recall of supraspan word-lists. Free recall tasks produce several benchmark memory phenomena, like the U-shaped serial position curve, reflecting enhanced memory for early and late list items. To account for empirical data, including primacy and recency as well as contiguity effects, we propose here a neurobiologically based neural network model that unifies short- and long-term forms of memory and challenges both the standard view of working memory as persistent activity and dual-store accounts of free recall. Rapidly expressed and volatile synaptic plasticity, modulated intrinsic excitability, and spike-frequency adaptation are suggested as key cellular mechanisms underlying working memory encoding, reactivation and recall. Recent findings on the synaptic and molecular mechanisms behind early LTP and on spiking activity during delayed-match-to-sample tasks support this view. 相似文献
55.
Bethany M. Henrick Lucie Rodriguez Tadepally Lakshmikanth Christian Pou Ewa Henckel Aron Arzoomand Axel Olin Jun Wang Jaromir Mikes Ziyang Tan Yang Chen Amy M. Ehrlich Anna Karin Bernhardsson Constantin Habimana Mugabo Ylva Ambrosiani Anna Gustafsson Stephanie Chew Heather K. Brown Petter Brodin 《Cell》2021,184(15):3884-3898.e11
- Download : Download high-res image (173KB)
- Download : Download full-size image
56.
A taxonomic reappraisal of the European Daphnia longispina complex (Crustacea, Cladocera, Anomopoda)
Adam Petrusek Anders Hobæk Jens Petter Nilssen Morten Skage Martin ČErný Nora Brede Klaus Schwenk 《Zoologica scripta》2008,37(5):507-519
The Daphnia longispina complex contains some of the most common water flea species in the northern hemisphere, and has been a model organism for many ecological and evolutionary studies. Nevertheless, the systematics and nomenclature of this group, in particular its Palaearctic members, have been in flux for the past 150 years; this hinders the correct interpretation of scientific results and promotes the erroneous use of species names. We revise the systematics of this species complex based on mitochondrial sequence variation (12S rDNA and COI) of representative populations across Europe, with a special focus on samples from type localities of the respective taxa. Combining genetic evidence and morphological assignments of analysed individuals, we propose a comprehensive revision of the European members of the D. longispina complex. We show that D. hyalina and D. rosea morphotypes have evolved several times independently, and we find no evidence to maintain these morphotypes as distinct biological species. Alpine individuals described as D. zschokkei are conspecific with the above-mentioned lineage. We suggest that this morphologically and ecologically plastic but genetically uniform hyalina–rosea–zschokkei clade should be identified as D. longispina (O. F. Müller, 1776). The valid name of Fennoscandian individuals labelled D. longispina sensu stricto in the recent literature is D. lacustris G. O. Sars, 1862. Additionally, we discovered another divergent lineage of this group, likely an undescribed species, in southern Norway. Our results present a solution for several prevailing taxonomic problems in the genus Daphnia , and have broad implications for interpretation of biogeographical patterns, and ecological and evolutionary studies. 相似文献
57.
Martin Breitbach Kenichi Kimura Tiago C. Luis Christopher J. Fuegemann Petter S. Woll Michael Hesse Raffaella Facchini Sarah Rieck Katarzyna Jobin Julia Reinhardt Osamu Ohneda Daniela Wenzel Caroline Geisen Christian Kurts Wolfgang Kastenmüller Michael Hölzel Sten E.W. Jacobsen Bernd K. Fleischmann 《Cell Stem Cell》2018,22(2):262-276.e7
58.
Medium-sized predators sometimes switch to alternative prey species as their main prey declines. Our objective of this study was to test the alternative prey hypothesis for a medium sized predator (red fox, Vulpes vulpes ), a small cyclically fluctuating main prey (microtine voles) and larger alternative prey (roe deer fawns, Capreolus capreolus ). We used long-term time series (28 years) on voles, red fox and roe deer from the Grimsö Wildlife Research Area (59°40'N, 15°25'E) in south-central Sweden to investigate interspecific relationships in the annual fluctuations in numbers of the studied species. Annual variation in number of roe deer fawns in autumn was significantly and positively related to vole density and significantly and negatively related to the number of fox litters in the previous year. In years of high vole density, predation on roe deer fawns was small, but in years of low vole density predation was more severe. The time lag between number of fox litters and predation on fawns was due to the time lag in functional response of red fox in relation to voles. This study demonstrates for the first time that the alternative prey hypothesis is applicable to the system red fox, voles and roe deer fawns. 相似文献
59.
Espen Burum-Auensen Paula M De Angelis Aasa R Schj?lberg Katherine L Kravik Marit Aure Ole Petter F Clausen 《The journal of histochemistry and cytochemistry》2007,55(5):477-486
The spindle checkpoint, the primary mechanism to ensure that two daughter cells receive the same amount of DNA, is compromised in many malignant tumors and has been implicated as a contributor to aneuploidy and carcinogenesis. The extent of expression and subcellular localization of the spindle proteins Aurora A, Mad2, and BUBR1 varies considerably in different immunohistochemical (IHC) reports from archival tumor tissues. Given the conflicting reports in the literature about the localization of these proteins, we examined the subcellular localization of Aurora kinase A, Mad2, and BUBR1 in normal and cancerous human tissues by IHC. In normal tissues, Aurora A was mainly localized to the nucleus when monoclonal or purified polyclonal antibodies were used, and Mad2 was localized to the nucleus, whereas BUBR1 was localized to the cytoplasm. In malignant tissues, Aurora A showed additional staining in the cytoplasm in the majority of tumors analyzed. Furthermore, BUBR1 was also localized to both the nucleus and cytoplasm in a significant fraction of tumors. Subcellular localization of Mad2 was similar in normal and malignant tissues. Thus, the validity of some earlier IHC studies of Aurora A, Mad2, and BUBR1 should be reconsidered, indicating that high-quality antibodies and a high-alkaline antigen-retrieval technique are required to achieve optimal results. We conclude that the subcellular localizations of these spindle proteins are different, although they have overlapping biological functions, and that Aurora A and BUBR1 undergo a shift in the subcellular localization during malignant transformation. 相似文献
60.
T. Tvrdik Suzanne Marcus Sai-Mei Hou Susann Fält Peri Noori Natalia Podlutskaja Folker Hanefeld Petter Strømme Bo Lambert 《Human genetics》1998,103(3):311-318
Mutations identified in the hypoxanthine phosphoribosyltransferase (HPRT) gene of patients with Lesch-Nyhan (LN) syndrome
are dominated by simple base substitutions. Few hotspot positions have been identified, and only three large genomic rearrangements
have been characterized at the molecular level. We have identified one novel mutation, two tentative hot spot mutations, and
two deletions by direct sequencing of HPRT cDNA or genomic DNA from fibroblasts or T-lymphocytes from LN patients in five
unrelated families. One is a missense mutation caused by a 610C→T transition of the first base of HPRT exon 9. This mutation
has not been described previously in an LN patient. A nonsense mutation caused by a 508C→T transition at a CpG site in HPRT
exon 7 in the second patient and his younger brother is the fifth mutation of this kind among LN patients. Another tentative
hotspot mutation in the third patient, a frame shift caused by a G nucleotide insertion in a monotonous repeat of six Gs in
HPRT exon 3, has been reported previously in three other LN patients. The fourth patient had a tandem deletion: a 57-bp deletion
in an internally repeated Alu-sequence of intron 1 was separated by 14 bp from a 627-bp deletion that included HPRT exon 2 and was flanked by a 4-bp repeat.
This complex mutation is probably caused by a combination of homologous recombination and replication slippage. Another large
genomic deletion of 2969 bp in the fifth patient extended from one Alu-sequence in the promoter region to another Alu-sequence of intron 1, deleting the whole of HPRT exon 1. The breakpoints were located within two 39-bp homologous sequences,
one of which overlapped with a well-conserved 26-bp Alu-core sequence previously suggested as promoting recombination. These results contribute to the establishment of a molecular
spectrum of LN mutations, support previous data indicating possible mutational hotspots, and provide evidence for the involvement
of Alu-mediated recombination in HPRT deletion mutagenesis.
Received: 21 April 1998 / Accepted: 16 July 1998 相似文献