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排序方式: 共有125条查询结果,搜索用时 15 毫秒
21.
Adjele Wilson James N. Kinney Petrus H. Zwart Claire Punginelli Sandrine D'Haene Fran?ois Perreau Michael G. Klein Diana Kirilovsky Cheryl A. Kerfeld 《The Journal of biological chemistry》2010,285(24):18364-18375
The photoprotective processes of photosynthetic organisms involve the dissipation of excess absorbed light energy as heat. Photoprotection in cyanobacteria is mechanistically distinct from that in plants; it involves the orange carotenoid protein (OCP), a water-soluble protein containing a single carotenoid. The OCP is a new member of the family of blue light-photoactive proteins; blue-green light triggers the OCP-mediated photoprotective response. Here we report structural and functional characterization of the wild type and two mutant forms of the OCP, from the model organism Synechocystis PCC6803. The structural analysis provides high resolution detail of the carotenoid-protein interactions that underlie the optical properties of the OCP, unique among carotenoid-proteins in binding a single pigment per polypeptide chain. Collectively, these data implicate several key amino acids in the function of the OCP and reveal that the photoconversion and photoprotective responses of the OCP to blue-green light can be decoupled. 相似文献
22.
23.
Yinghong Hu Eline M. Hutter Philipp Rieder Irene Grill Jonas Hanisch Meltem F. Aygüler Alexander G. Hufnagel Matthias Handloser Thomas Bein Achim Hartschuh Kristofer Tvingstedt Vladimir Dyakonov Andreas Baumann Tom J. Savenije Michiel L. Petrus Pablo Docampo 《Liver Transplantation》2018,8(16)
Adding cesium (Cs) and rubidium (Rb) cations to FA0.83MA0.17Pb(I0.83Br0.17)3 hybrid lead halide perovskites results in a remarkable improvement in solar cell performance, but the origin of the enhancement has not been fully understood yet. In this work, time‐of‐flight, time‐resolved microwave conductivity, and thermally stimulated current measurements are performed to elucidate the impact of the inorganic cation additives on the trap landscape and charge transport properties within perovskite solar cells. These complementary techniques allow for the assessment of both local features within the perovskite crystals and macroscopic properties of films and full devices. Strikingly, Cs‐incorporation is shown to reduce the trap density and charge recombination rates in the perovskite layer. This is consistent with the significant improvements in the open‐circuit voltage and fill factor of Cs‐containing devices. By comparison, Rb‐addition results in an increased charge carrier mobility, which is accompanied by a minor increase in device efficiency and reduced current–voltage hysteresis. By mixing Cs and Rb in quadruple cation (Cs‐Rb‐FA‐MA) perovskites, the advantages of both inorganic cations can be combined. This study provides valuable insights into the role of these additives in multiple‐cation perovskite solar cells, which are essential for the design of high‐performance devices. 相似文献
24.
Cells that are part of a multicellular structure are typically embedded in an extracellular matrix, which is produced by the community members. These matrices, the composition of which is highly diverse between different species, are typically composed of large amounts of extracellular polymeric substances, including polysaccharides, proteins, and nucleic acids. The functions of all these matrices are diverse: they provide protection, mechanical stability, mediate adhesion to surfaces, regulate motility, and form a cohesive network in which cells are transiently immobilized. In this review we discuss the role of matrix components produced by streptomycetes during growth, development and attachment. Compared to other bacteria it appears that streptomycetes can form morphologically and functionally distinct matrices using a core set of building blocks. 相似文献
25.
Shih-Hsin Chang Petrus Tang Cheng-Hung Lai Ming-Ling Kuo Lian-Chen Wang 《Memórias do Instituto Oswaldo Cruz》2013,108(6):699-706
Angiostrongylus cantonensis is an important causative agent of
eosinophilic meningitis and eosinophilic meningoencephalitis in humans.
MicroRNAs (miRNAs) are small non-coding RNAs that participate in a wide range of
biological processes. This study employed a deep-sequencing approach to study
miRNAs from young adults of A. cantonensis. Based on 16,880,456
high-quality reads, 252 conserved mature miRNAs including 10 antisense miRNAs
that belonging to 90 families, together with 10 antisense miRNAs were identified
and characterised. Among these sequences, 53 miRNAs from 25 families displayed
50 or more reads. The conserved miRNA families were divided into four groups
according to their phylogenetic distribution and a total of nine families
without any members showing homology to other nematodes or adult worms were
identified. Stem-loop real-time polymerase chain reaction analysis of
aca-miR-1-1 and aca-miR-71-1 demonstrated that their level of expression
increased dramatically from infective larvae to young adults and then decreased
in adult worms, with the male worms exhibiting significantly higher levels of
expression than female worms. These findings provide information related to the
regulation of gene expression during the growth, development and pathogenesis of
young adults of A. cantonensis. 相似文献
26.
Summary The hydrolysis by 1,3-specific lipases (Humicola lanuginosa, Mucor miehei, Rhizopus delemar andRhizopus javanicus) of the highly symmetric, high molecular weight triglycerides fromCrambe abyssinica (Crambe) seed oil is studied in an AOT-stabilized microemulsion system. Enzyme kinetic data shows that, of the lipases studied,Rhizopus javanicus lipases exerts the highest hydrolytic activity towards this new seed oil. 相似文献
27.
Nitric oxide (NO) can induce acute pain in humans and plays an important role in pain sensitization caused by inflammation and injury in animal models. There is evidence that NO acts both in the central nervous system via a cyclic GMP pathway and in the periphery on sensory neurons through unknown mechanisms. It has recently been suggested that TRPV1 and TRPA1, two polymodal ion channels that sense noxious stimuli impinging on peripheral nociceptors, are activated by NO in heterologous systems. Here, we investigate the relevance of this activation. We demonstrate that NO donors directly activate TRPV1 and TRPA1 in isolated inside-out patch recordings. Cultured primary sensory neurons display both TRPV1- and TRPA1-dependent responses to NO donors. BH4, an essential co-factor for NO production, causes activation of a subset of DRG neurons as assayed by calcium imaging, and this activation is at least partly dependent on nitric oxide synthase activity. We show that BH4-induced calcium influx is ablated in DRG neurons from TRPA1/TRPV1 double knockout mice, suggesting that production of endogenous levels of NO can activate these ion channels. In behavioral assays, peripheral NO-induced nociception is compromised when TRPV1 and TRPA1 are both ablated. These results provide genetic evidence that the peripheral nociceptive action of NO is mediated by both TRPV1 and TRPA1. 相似文献
28.
Petrusová M Vojtech M Pribulová B Lattová E Matulová M Poláková M BeMiller JN Kren V Petrus L 《Carbohydrate research》2006,341(12):2019-2025
Acid-catalysed methanolysis of 3,4,5,6-tetra-O-acetyl-1,2-dideoxy-l-arabino-hex-1-enitol proceeds via a cascade set of consecutive reactions resulting in its regiospecific conversion to a mixture of alpha- and beta-C-L-arabinofuranosylmethanal dimethyl acetals and a mixed internal methyl acetal. Structures of the final products of the overall process provide unique evidence that a kinetically controlled, five-membered-ring closure precedes a six-membered-ring closure in reversible systems capable of giving both five-membered and six-membered all-sp3-atom rings. Determination of the reaction intermediate enabled extension of the Nef reaction to C-glycosylnitromethanes. Protonated aci-nitro forms of C-glycosylnitromethanes that are resistant to the Nef reaction in aqueous acidic media undergo a modified Nef reaction in acidified methanol, and the corresponding C-glycosylmethanal dimethyl acetals with alpha-L-arabinopyranosyl, beta-D-glucopyranosyl, beta-D-galactopyranosyl, beta-D-mannopyranosyl and beta-L-rhamnopyranosyl configurations were obtained in moderate yields. 相似文献
29.
Malovichko OL Petrus AS Krysko AA Kabanova TA Andronati SA Karaseva TL Kiriyak AV 《Bioorganic & medicinal chemistry letters》2006,16(20):5294-5297
The novel fibrinogen receptor antagonists containing fragments of 7-amino-1,2,3,4-tetrahydroisoquinoline and isophthalic acids were synthesized and successfully tested for their ability to inhibit platelet aggregation in vitro and to block FITC-Fg binding to alpha(IIb)beta(3) on washed human platelets. 相似文献
30.
Chimeric G proteins, obtained by exchanging their C-terminal portion for that of a G protein from an unrelated class, drive the receptor selectivity to that corresponding to the introduced G protein domain. The 2A-adrenoceptor (2AAR), which yielded an efficacious and weak [35S]GTPS binding response by respectively G o and G i3 protein, was investigated in CHO-K1 cells co-expressing chimeric G proteins for which the six last C-terminal amino acids between G o and G i3 proteins, and reciprocally, were permuted. Activation of the chimeric G o / i3 protein was highly efficient whereas the G i3 / o protein yielded a weak stimulation. These [35S]GTPS binding responses were not different from their parental wild-type G o and G i3 proteins. Similar results were obtained with an 2AAR carrying a facilitating Thr373Lys mutation in a putative G protein interaction domain. These data indicate that the six terminal G o protein amino acids do not constitute a major 2AAR interaction domain for G protein activation. 相似文献