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121.
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Kramer Gerard F.H. Jurrius Otto de Rijk Theo C. Derksen Johannes T.P. Cuperus F. Petrus 《Biotechnology Techniques》1997,11(5):293-295
Defatted soy flour with a membrane-bound epoxide hydrolase wasused for the conversion on a preparative scale of9,10-epoxy-octadecanoic acid, 9,10-epoxy-12-hydroxy-octadecanoicacid and 12,13-epoxy-9-octadecenoic acid (vernolic acid) intotheir 1,2-diols. The products might be used in coatings, lubricantsor for the synthesis of fine chemicals. 相似文献
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A modified overlap extension technique for the creation of chimeric genes is described: the method consists in three PCR steps. The first step is a conventional PCR reaction, in which oligonucleotide primers are partially complementary at their 5' ends to the adjacent fragments that are fused to create the chimer. The second PCR step consists in the fusion of the PCR fragments generated in the first step using the complementary extremities of the primers. The third step corresponds to the PCR amplification of the fusion product. The final PCR product is a chimeric gene built up with the different amplified PCR fragments. The technique is illustrated by the construction of a chimeric 5- hydroxytryptamine (5-HT, serotonin)1B/D receptor by combining one part of the human 5-HT1B (h5-HT1B) and two parts of the h5-HT1D receptor gene. The chimeric gene expressed in Cos-7 cells yielded similar binding properties as the wild type h5-HT1D receptor. © Rapid Science Ltd. 1998 相似文献
125.
Temperature and pH had only a slight effect on the astaxanthin content of a Phaffia rhodozyma mutant, but influenced the maximum specific growth rate and cell yield profoundly. The optimum conditions for astaxanthin production were 22°C at pH 5.0 with a low concentration of carbon source. Astaxanthin production was growth-associated, and the volumetric astaxanthin concentration gradually decreased after depletion of the carbon source. The biomass concentration decreased rapidly during the stationary growth phase with a concomitant increase in the cellular content of astaxanthin. Sucrose hydrolysis exceeded the assimilation rates of D-glucose and D-fructose and these sugars accumulated during batch cultivation. D-Glucose initially delayed D-fructose uptake, but D-fructose utilization commenced before glucose depletion. In continuous culture, the highest astaxanthin content was obtained at the lowest dilution rate of 0.043 h–1. The cell yield reached a maximum of 0.48 g cells·g–1 glucose utilized between dilution rates of 0.05 h–1 and 0.07 h–1 and decreased markedly at higher dilution rates.
Correspondence to: J. C. Du Preez 相似文献