Lamellar bodies in the epithelial bronchiolar cells in the mouse

Summary Lamellar bodies are described in the non-ciliated epithelial bronchiolar cells of the normal mouse lung. They are constituted of smooth concentric membranes, with a cytoplasmic center. They are related to mitochondria. They seem to belong to smooth endoplasmic reticulum. An origin from Golgi elements is discussed. Acknowledgment. This work was supported by Grant No 69088 of Conseil de la Recherche Médicale du Québec.     

Use of Carbowax as a high molecular weight nonprotein substance for cultivation of lymphoid cell and study of their morphology

Lymphoid cells prepared from rabbit popliteal lymph nodes were cultivated in medium in which the high molecular weight component was replaced by Carbowax 20 M. This substance, in 0.2% concentration, proved to be a substitute for the high molecular weight component in short-term cultivation, and the viability results were similar to those in medium containing normal rabbit serum or 0.5% human serum albumin. In addition, mitoses and the formation of large basophilic cells resembling the blastoid cells induced during cultivation with phytohaemagglutinin were observed in this medium. The results are discussed.     

Secondary responsein vitro to antigen ΦX 174 phage

In a model secondary reactionin vitro, a correlation was demonstrated between the size of the antigen dose used for the prestimulation of spleen tissue donors and the type of antibodies formed in the anamnestic reaction. After a small dose of antigen ΦX 174, the antibody response three days after prestimulation was of the 19 S (IgM) type, but later secondary contactin vitro (after four months) did not produce a 19 S anamnestic reaction. After large primary doses of antigen, a short interval between primary and secondary contact led to the formation of 19 and 7 S type antibodies, while after a long interval only 7 S (IgG) type antibodies were formed. The results are discussed in relation to differences in the size of the antigen dose needed to induce short-term 19 S and long-term 7 S immunological memory.     

A Comparative study on the reactions for protein sulphydryl,carboxyl and tyrosine in plant nuclei

V této práci byly aplikovány na rostlinných objektech reakce s β-oxynaftyl-merkurichloridem a p-nitrobromacetofenonem, popsané k pr?kazu bílkovinných SH na materiálu ?ivo?i?ném. V rostlinných meristematických buňkách obě tyto techniky stejně jako DDD¹ a RSR¹ jeví vět?inou intensivněj?í zbarvení jader ne? cytoplasmy. Rovně? po aplikaci reakcí na bílkovinné karboxyly a tyrosin je jádro intensivněji zbarveno ne? cytoplasma. Z toho lze soudit, ?e rozdíly v intensitě zbarvení jádra a cytoplasmy v meristematických buňkách rostlinných technikami k pr?kazu bílkovinných SH jsou zp?sobeny spí? rozdílem v celkové koncentraci bílkovin ne? zv??ením podílu cysteinu v jaderných bílkovinách.     

List of publications on human biometeorology in Czechoslovakia (period 1920–1960)

International Journal of Biometeorology -     

Relation of some M1 characters to the frequency of M2 mutants inArabidopsis thaliana

The relation of the M₁ root length and the frequency of M₁ chlorophyll chimeras to the sterility grade and to the frequency of M₂ mutants ofArabidopsis thaliana is demonstrated.     

Estimation of the mean floristic similarity between and within sets of vegetational relevés

Various possibilities of usingSörensen's coefficient of floristic similarity in plant sociology are summarized. A new formula (11), derived from that bySörensen and based on constancy values, is suggested in order to calculate the mean floristic similarity within a set of relevés.     

Estimation of the mean floristic similarity between and within sets of vegetational relevés

Various possibilities of usingSörensen’s coefficient of floristic similarity in plant sociology are summarized. A new formula (11). derived from that bySörensen and based on constancy values, is suggested in order to calculate the mean floristic similarity within a set of relevés.     

The genusSaccharomyces (Meyen) Reess

The authors submit a taxonomic evaluation of an intermediate group of strains between the speciesSaccharomyces carlsbergensis Hansen andSaccharomyces cerevisiae Hansen. The material consisted of atypical strains of “bottom” brewer’s yeasts and the synonymous strainsSaccharomyces monacensis Hansen andSaccharomyces mandshuricus Saito. It was found that there were two different serological types in the speciesSaccharomyces carlsbergensis, one of which was characterized by the presence of antigen “C” and was typical for this species, while the other possessed antigen “M” and was grouped roundSaccharomyces monacensis. This second serological type merges with a group of strains which gives only one third fermentation of raffinose, so that it is actually an intermediate betweenSaccharomyces cerevisiae Hansen andSaccharomyces carlsbergensis Hansen and indicates the course of progressive development from the former species to the latter. No close similarity was found betweenSaccharomyces mandshuricus Saito and some of the strains of the transitional group or typical representatives of the two main species, and the authors therefore consider that there is some obscurity as to its synonymity withSaccharomyces carlsbergensis.