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991.
Peter Hays 《BMJ (Clinical research ed.)》1996,313(7068):1338
992.
993.
Bramah N. Singh James N. Weiss Koonlawee Nademanee John H. Wittig Peter Guzy 《The Western journal of medicine》1984,141(5):649-665
An edited summary of an Interdepartmental Conference arranged by the Department of Medicine, UCLA School of Medicine, Los Angeles. William M. Pardridge, MD, Associate Professor of Medicine, is Director of Conferences.This study was supported in part by grants from the Public Health Service; the National Institutes of Health (HL-23970, 1978-1981); the Medical Research Service of the Veterans Administration, and the American Heart Association, the Greater Los Angeles Affiliate. 相似文献
994.
995.
996.
James M. Bullock Stephen J. Galsworthy Pablo Manzano Peter Poschlod Carsten Eichberg Katherine Walker Matthias C. Wichmann 《Oikos》2011,120(8):1201-1208
Studies of external seed transport on animals usually assume that the probability of detachment is constant, so that seed retention should show a simple exponential relationship with time. This assumption has not been tested explicitly, and may lead to inaccurate representation of long distance seed dispersal by animals. We test the assumption by comparing the fit to empirical data of simple, two‐parameter functions. Fifty‐two data sets were obtained from five published studies, describing seed retention of 32 plant species on sheep, cattle, deer, goats and mice. Model selection suggested a simple exponential function was adequate for data sets in which seed retention was followed for short periods ( <48 h). The data gathered over longer periods (49–219 days) were best described by the power exponential function, a form of the stretched exponential which allows a changing dropping rate. In these cases the power exponential showed that seed dropping rate decreased with time, suggesting that seeds vary in attachment, with some seeds becoming deeply buried or wound up in the animal's coat. Comparison of fitted parameters across all the data sets also confirmed that seeds with adhesive structures have lower dropping rates than those without. We conclude that the seed dropping rate often changes with time during external transport on animals and that the power exponential is an effective function to describe this change. We advise that, to analyse seed dropping rates adequately, retention should be measured over reasonable time periods – until most seeds are dropped – and both the simple and power exponential functions should be fitted to the resulting data. To increase its utility, we provide functions describing the seed dropping rate and the dispersal kernel resulting from the power exponential relationship. 相似文献
997.
998.
Viktor Y. Butnev R. Russell Gotschall Vanda L. Baker William T. Moore Peter W. Gout George R. Bousfield 《Journal of Protein Chemistry》1996,15(5):413-426
Glycosylated equine prolactin (G-ePRL) and nonglycosylated ePRL were purified to homogeneity from side fractions obtained during isolation of LH/FSH from horse pituitaries. Both PRL forms were isolated together in high yield by the isolation procedure used for glycosylated porcine PRL/(G-pPRL) and pPRL, involving acetone extraction/precipitation, NaCl and isoelectric precipitation, and gel filtration. Purification of G-ePRL required additional Con A chromatography. The N-terminal amino acid sequencing for 32 cycles of G-ePRL and ePRL resulted in sequences identical to the known primary structure of ePRL. Based on MALDI mass spectrometry analysis and SDS-PAGE mobilities,G-ePRL and ePRL had estimated molecular weights of 25,000 and 23,000 Da, respectively. G-ePRL displayed only 60% of the immunoreactivity of ePRL in homologous radioimmunoassay. Using the Nb2 lymphoma cell bioassay, ePRL was found to have about l/30th the mitogenic activity of bovine PRL; G-ePRL was approximately l/10th as active as ePRL. Glycosylation of G-ePRL at Asn31 was confirmed by isolation and sequence analysis of an enzymatically derived G-ePRL glycopeptide spanning residues 29–37. Monosaccharide compositions of intact G-ePRL and this glycopeptide were very similar (Man3, GlcNAc2, GalNAc1, Fuc0.6, Gal0.2, NeuAc0.15) and resembled that of G-pPRL. The glycopeptide contained one sulfate residue as determined by ion chromatography after acid hydrolysis, indicating the presence of a sulfated monosaccharide. Comparative carbohydrate analysis of G-ePRL and other G-PRL preparations suggests that the functionally significant Asn31 carbohydrate unit is a fucosylated complex mono- and/or biantennary oligosaccharide terminating with a sulfated GalNAc residue and two or three Man residues. 相似文献
999.
Peter E.M. Gibbs Irwin M. Freedberg 《Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression》1982,696(2):124-133
A messenger RNA fraction from guinea-pig skin sediments on sucrose gradients at approx. 19 S and codes for keratin polypeptides in a messenger-dependent reticulocyte lysate system. Partial purification of this fraction was achieved by two cycles of chromatography on oligo(dT)-cellulose, followed by two cycles of sucrose gradient centrifugation. The identities of the protein products as keratins were established by co-electrophoresis on sodium dodecyl sulfate-polyacrylamide gels, by peptide mapping, and by co-electrophoresis on two-dimensional polyacrylamide gels. All of the epidermal keratin polypeptides which are present in vivo are synthesized in vitro under the direction of this messenger. Fractionation of the messenger indicates that each different polypeptide is the product of a single mRNA species, and that no keratin is formed by proteolytic processing of higher molecular weight species or by polymerization of smaller precursors. Post-translational changes such as phosphorylation, which are known to occur in vivo, cannot be identified in the reticulocyte lysate system. Translation of these keratin messenger species is strongly inhibited by 7-methylguanosine 5′-triphosphate, indicating that the molecules have a ‘capped’ 5′-terminus. 相似文献
1000.
Peter H. Raven 《Biotropica》2011,43(5):521-523
Conservation initiatives that have worked well in temperate and developed regions have often been applied in the tropics but with only limited success. Part of this failure is due to top–down conservation planning that has been conducted without taking local socio‐economic considerations into adequate account. Here, we argue that conservation approaches would benefit from a deeper understanding of human–nature interactions. 相似文献