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111.
112.
Peter A. Siver 《Nordic Journal of Botany》2002,22(1):115-121
Two new species of Mallomonas, M. ocalensis and M. caerula , are described from a clearwater, acidic and oligotrophic waterbody in the Ocala National Forest, Florida, U.S.A. Mallomonas ocalensis , in the Series Doignonianae of the Section Torquatae, consists of relatively small cells with scales that have a series of parallel shield ribs with alternating thicknesses, collar scales with very short bristles and posterior scales with small protruding spines. Mallomonas ocalensis is most similar to Mallomonas dickii , but is distinguished from the latter species based on the highly variable thicknesses of the shield ribs and significantly larger scales. Mallomonas caerula , in the Series Mallomonas of the Section Mallomonas, consists of relatively large cells that bear large domed and dorneless scales, and long, robust and ribbed bristles. Bristles with and without helmets can be found. The shield, especially the distal portion, and the posterior flange of scales consists of series of more or less parallel ribs. Mallomonas caerula appears to be most closely related to Mallomonas acaroides var. acaroides and Mallomonas acaroides var. muskokana , but can be distinguished from the latter taxa on the basis of scale type, scale structure and bristle morphology. Both new species were found in the plankton and surface sediments of Blue Sink Pond. 相似文献
113.
Plasma endotoxin and concentrations of stable metabolites of prostacyclin, thromboxane A2, and prostaglandin E2 in postpartum dairy cows 总被引:1,自引:0,他引:1
The presence of endotoxin in plasma and patterns of stable metabolites of prostacyclin (PC), thromboxane A2 (TXA2) and prostaglandin E2 (PGE2) were determined during the first postpartum estrous cycles in sixteen dairy cows. These included 8 cows with uterine infections which exhibited shortened luteal phases (SC) and 8 cows which had normal luteal phases (NC) after the first post partum ovulations. Endotoxin was consistently detected in all SC cows during the abbreviated estrous cycles while plasma samples of NC cows were free of endotoxin. Plasma concentrations of TXA2 metabolite was higher in SC cows (p less than 0.05) (1785-3452 pg/ml) compared to NC cows (723-1240 pg/ml). Similarly, plasma concentrations of PC metabolite was higher in SC cows (p less than 0.07) (423-1847 pg/ml) compared to NC cows (159-325 pg/ml). In contrast, plasma concentrations of PGE2 metabolite was higher in NC cows (p less than 0.05) (850-2219 pg/ml) compared to SC cows (455-628 pg/ml). The results of this study suggest that postpartum uterine infections mediate the release of prostaglandins from the uteri by means of the endotoxin and endotoxin appears to stimulate selectively the production of PC and TXA2 favoring early demise of corpora lutea formed after first postpartum ovulations in dairy cows. 相似文献
114.
Mauro S. Sandrin K. Erin Lovering George Tachas Peter R. Collins Ian F. C. McKenzie 《Immunogenetics》1987,25(5):279-283
Human DNA was transfected into mouse L cells and tk+ HuLy-m2+ (= CD7+) transfectants isolated after growth in hypoxanthine, aminopterin, thymidine medium and repeated cloning. After several cycles of transfection, > 90% of HuLy-m2+ L cells could be detected, by rosetting and by cytofluorography, which showed the transfectants to have a density of CD7 two to five times that found on peripheral blood lymphocytes. Despite this, the 37 kd CD7+ dimer could only be identified with difficulty using cell-surface radioiodination and sodium dodecyl sulfate-polyacrylamide gel electrophoresis techniques. An antiserum was produced (C3H anti-HuLy-m2+ L cells) which, after absorption, was shown to react with HuLy-m2 antigens present on human thymocytes and lymphocytes and on CD7+ transfected L cells.Abbreviations BSA
bovine serum albumin
- DME
Dulbecco's modified Eagle's medium
- EDTA
ethylenediamine-tetraacetate
- HAT
hypoxanthine, aminopterin, thymidine
- HSV
herpes simplex virus
- PBL
peripheral blood lymphocyte
- PBS
phosphate-buffered saline
- RFC
rosette-forming cell
- SDS-PAGE
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
- tk
thymidine kinase 相似文献
115.
Native bovine parathyroid hormone (bPTH) was found to be readily cleaved with human leukocyte elastase to yield the fragments bPTH(1–41) and bPTH(42–84). These were then isolated by reverse-phase HPLC and characterised by gas-phase sequencing and amino acid analysis. The biological activities of these fragments were assessed in an adenylate cyclase bioassay using the rat osteosarcoma cell line UMR106. bPTH(1–41) was found to have approximately twice the molar potency of the native hormone from which it was derived, bPTH(42–84) had no biological activity and did not modulate the adenylate cyclase response to these cells to the native hormone. The possible physiological significance of these observations is discussed. 相似文献
116.
117.
Big Moose Basin: simulation of response to acidic deposition 总被引:2,自引:1,他引:1
George F. Davis John J. Whipple Steven A. Gherini Carl W. Chen Robert A. Goldstein Arland H. Johannes Peter W.H Chan Ronald K. Munson 《Biogeochemistry》1987,3(1-3):141-161
The ILWAS model has been enhanced for application to multiple-lake hydrologic basins. This version of the model has been applied to the Big Moose basin, which includes Big Moose Lake and its tributary streams, lakes, and watersheds. The basin, as defined, includes an area of 96 km2, with over 20 lakes and ponds, and 70 km of streams. Hydrologic and chemical calibrations have been made using data from seven sampling stations. When total atmospheric sulfur loading to the basin is halved, the model predicts, after four years of simulation, a decreasing sulfate concentration and to a lesser extent a rising alkalinity at Big Moose Lake outlet. At the end of four years, the results show an increase in pH of 0.1 to 0.5 pH units depending upon season. 相似文献
118.
Denitrification with methanol in the presence of high salt concentrations and at high pH levels 总被引:3,自引:0,他引:3
Jan Peter van der Hoek Paul J. M. Latour Abraham Klapwijk 《Applied microbiology and biotechnology》1987,27(2):199-205
Summary In the combined ion exchange/biological denitrification process for nitrate removal from ground water, in which nitrate is removed by ion exchange, the resins are regenerated in a closed circuit by a biological denitrification reactor. This denitrification reactor eliminates nitrate from the regenerant. Methanol is used as electron donor for biological denitrification. To obtain sufficient regeneration of the resins within a reasonable time, high NaCl or NaHCO3 concentrations (10–30 g/l) in the regenerant are necessary. High NaHCO3 concentrations affected the biological denitrification in three ways: a) a slight decrease in denitrification capacity (30%) was observed; b) the yield coefficient and CH3OH/NO3
-–N ratio decreased. When high NaHCO3 concentrations (above 10g NaHCO3/l) were used, the yield coefficient was 0.10–0.13 g VSS/g NO3
-–N and the CH3OH/NO3
-–N ratio was 2.00–2.03 g/g; c) high NaHCO3 concentrations influenced nitrite production. Nitrite is an intermediate product of biological denitrification and with rising NaHCO3 concentrations nitrite accumulation was suppressed. This was explained by the effect of high NaHCO3 concentrations on the pH in the microenvironment of the denitrifying organisms. High NaCl concentrations also resulted in a slight decrease in denitrification capacity, but the second and third effects were not observed in the presence of high NaCl concentrations.Although the pH in the regenerant will rise as a result of biological denitrification, the capacity of a denitrification reactor did not decrease significantly when a pH of 8.8–9.2 was reached. 相似文献
119.
Summary Washed microsomal preparations (100 000 xg sediment) from the yeast Sporopachydermia cereana that had been grown on trimethylamine N-oxide as sole nitrogen source catalysed the NAD(P)H-dependent reduction of trimethylamine N-oxide to trimethylamine. Under anaerobic conditions, this was the sole reaction product, but under aerobic conditions only small amounts of trimethylamine accumulated, most being further metabolized to methylamine and formaldehyde (no detectable dimenthylamine accumulated due to its rapid turnover). In the absence of NAD(P)H, no formation of amines or formaldehyde from trimethylamine N-oxide was detected. The trimethylamine N-oxide reductase activity was inhibited by quinacrine, Cu2+ ions, triethylamine N-oxide (apparent K
i 0.43 mM) and dimethyl sulphoxide (K
i 0.94 mM). Chlorate and nitrate failed to inhibit the enzyme. The K
m for trimethylamine N-oxide was 29 M. Triethylamine N-oxide was also reduced by the microsomal preparation with the formation of acetaldehyde, and this reduction was sensitive to the same inhibitors as trimethylamine N-oxide, suggesting that both amine oxides are metabolized by the same enzyme(s). It is concluded that trimethylamine N-oxide is metabolized in this yeast via an NAD(P)H-dependent reductase.Abbreviations TMAO
triemthylamine N-oxide 相似文献
120.
Nigel W. Kerby Gordon W. Niven Peter Rowell William D. P. Stewart 《Applied microbiology and biotechnology》1987,25(6):547-552
Summary Mutant strains of the N2-fixing cyanobacterium bacterium Anabaena variabilis resistant to 6-fluorotryptophan or to ethionine were isolated. Many of these strains liberated amino acids into their media in the absence of 6-fluorotryptophan and ethionine. Nitrogenase activity was higher in mutant strains than in the parent strain. Mutant strains were immobilised in calcium alginate and sustained photoproduction of amino acids has been demonstrated.Abbreviations ETH
ethionine
- FT
6-fluorotryptophan
- Hepes
4-(2-hydroxyethyl)-1, piperazine ethanesulphonic acid
- PEP
phosphoenolpyruvate
- DAHP
3-deoxy-d-arabinoheptulosonate 7-phosphate
- chl a
chlorophyll a 相似文献