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951.
Tannin-rich extracts were painted on leaves of Q. emoryi (Fagaceae) that contained first instar larvae of a leaf miner, Cameraria sp. (Lepidoptera: Gracillariidae, Agrifoliella group), to test the role of tannins in increasing parasitism, while controlling for changes in protein content and physical damage. Miners from leaves that were treated with tannins had higher rates of parasitism by hymenopterans, but decreased mortality from other causes. The negative and positive effects of tannins balanced since overall larval survivorship was equivalent for leaf miners on tannin-enriched and control leaves. The tannin-rich extract also affected larval development because pupal weight was decreased for leaf miners in treated leaves. Tannins can have both adverse and beneficial effects on these phytophagous insects.
Résumé Des extraits riches en tanins ont été étalés sur des feuilles de Quercus emoryi contenant des chenilles de ler stade de Cameraria sp., pour contrôler l'influence du tanin dans l'accroissement du parasitisme sans modifier la teneur en protéines ni provoquer de dommages physiques. Les mineuses de feuilles traitées avaient des taux plus élevés de parasitisme par les hyménoptères, mais une diminution des autres causes de mortalité. Les effets négatifs et positifs des tanins se sont équilibrés, puisque la survie des mineuses était la même pour les feuilles traitées que pour les témoins. Les extraits riches en tanin ont agi sur le développement larvaire puisque le poids des chrysalides des mineuses de feuilles traitées était plus faible. Les tanins peuvent ainsi avoir des effets nuisibles et bénéfiques sur ces insectes phytophages.相似文献
952.
Effects of protein kinase C activators on germinal vesicle breakdown and polar body emission of mouse oocytes 总被引:4,自引:0,他引:4
Elayne A. Bornslaeger William T. Poueymirou Peter Mattei Richard M. Schultz 《Experimental cell research》1986,165(2):507-517
Protein phosphorylation mediated by cAMP-dependent protein kinase is instrumental in maintaining meiotic arrest of mouse oocytes. To assess whether protein phosphorylation mediated by calcium/phospholipid-dependent protein kinase (protein kinase C) might also inhibit the resumption of meiosis, we treated oocytes with activators of this enzyme. The active phorbol esters 12-O-tetra-decanoyl phorbol-13-acetate (TPA) and 4 beta-phorbol 12,13-didecanoate (4 beta-PDD) inhibited germinal vesicle breakdown (GVBD), as did a more natural activator of protein kinase, C, sn-1,2-dioctanoylglycerol (diC8). An inactive phorbol ester, 4 alpha-phorbol 12,13-didecanoate (4 alpha-PDD), did not inhibit GVBD. We then examined whether protein kinase C activators inhibit a step in the cAMP-modulated pathway that regulates resumption of meiosis. TPA did not inhibit the maturation-associated decrease in oocyte cAMP. Microinjected heat-stable protein inhibitor of cAMP-dependent protein kinase failed to induce GVBD in the presence of TPA. Both TPA and diC8 partially inhibited specific changes in oocyte phosphoprotein metabolism that are tightly correlated with resumption of meiosis; these agents also induced the apparent phosphorylation of specific oocyte proteins. These results suggest that protein kinase C activators may inhibit resumption of meiosis by acting distal to a decrease in cAMP-dependent protein kinase activity, but prior to changes in oocyte phosphoprotein metabolism that are presumably required for resumption of meiosis. Finally, we compared the effects of db-cAMP and protein kinase C activators on polar body emission following GVBD. TPA, 4 beta-PDD or diC8, but not 4 alpha-PDD or db-cAMP, inhibited polar body emission in a dose-dependent manner. The morphology and cytology of oocytes in which polar body emission was inhibited by TPA or 4 beta-PDD differed from that of oocytes treated with diC8. Thirty to 60% of the former were round in shape and exhibited a clump of chromosomes but no spindle; the remainder were distended in shape and exhibited a metaphase I spindle. All oocytes treated with diC8, however, were round, had dispersed chromosomes, and no spindle. These results suggest that, in contrast to resumption of meiosis, polar body emission is inhibited by activation of protein kinase C but not cAMP-dependent protein kinase. 相似文献
953.
The enzyme asparaginase, which hydrolyses asparagine to aspartic acid, inhibited cell-free protein synthesis by reticulocyte lysates. The inhibition was rapid and complete when sufficient enzyme was added but could be prevented or reversed by the addition of asparagine. The initial effect of asparaginase appears to be a block in polypeptide chain elongation due to asparagine deprivation, but there are some indications that prolonged incubation under these conditions may give rise to a secondary decrease in initiation of protein synthesis. 相似文献
954.
955.
We monitored an intertidal mussel (Mytilus edulis L.) population between June 1981 and June 1982 in the Eastern Scheldt estuary (S.W. Netherlands). Density and biomass of the population remained relatively constant over the study period. The shell length growth was described by a Gompertz growth curve. The parameters of this equation were estimated from a log-log-modified Ford-Walford plot of the growth-ring data. The slope of the relationship between animal weight and shell length is season-dependent, mainly due to the spawning cycle in larger mussels.Secondary production is estimated with the growth rate method. In the calculated growth rates the change in slope of the length-weight relationship is incorporated, as well as differences in length growth rates between summer and winter. Secondary production amounts to 156 g AFDW m–2a –1 (expressed per m2 of mussel bank). P:B is 0.50 a–1. The mussel productivity is probably a limiting factor for the density of overwintering Oystercatchers (Haematopus ostralegus). 相似文献
956.
957.
Methodologies are presented whereby the fresh organic carbon weight of formaldehyde preserved macrofaunal samples may be estimated. Length-organic carbon weight regressions were determined for the four numerically dominant bivalves in Narragansett Bay, Rhode Island (Nucula annulata, Yoldia limatula, Mulinia lateralis, and Pandora gouldiana) and one commercially important, but less abundant species (Mercenaria mercenaria). Constants were determined to convert the dry weight of preserved softbodied organisms (polychaetes, oligochaetes, amphipods, etc.) to fresh (unpreserved) organic carbon weight. These results can be used by investigators studying the energetics of benthic communities similar to those in Narragansett Bay. 相似文献
958.
Distinguishing between Nitrification and Denitrification as Sources of Gaseous Nitrogen Production in Soil 总被引:12,自引:2,他引:10
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The source of N2O produced in soil is often uncertain because denitrification and nitrification can occur simultaneously in the same soil aggregate. A technique which exploits the differential sensitivity of these processes to C2H2 inhibition is proposed for distinguishing among gaseous N losses from soils. Denitrification N2O was estimated from 24-h laboratory incubations in which nitrification was inhibited by 10-Pa C2H2. Nitrification N2O was estimated from the difference between N2O production under no C2H2 and that determined for denitrification. Denitrification N2 was estimated from the difference between N2O production under 10-kPa C2H2 and that under 10 Pa. Laboratory estimates of N2O production were significantly correlated with in situ N2O diffusion measurements made during a 10-month period in two forested watersheds. Nitrous oxide production from nitrification was most important on well-drained sites of a disturbed watershed where ambient NO3− was high. In contrast, denitrification N2O was most important on poorly drained sites near the stream of the same watershed. Distinction between N2O production from nitrification and denitrification was corroborated by correlations between denitrification N2O and water-filled pore space and between nitrification N2O and ambient NO3−. This technique permits qualitative study of environmental parameters that regulate gaseous N losses via denitrification and nitrification. 相似文献
959.
The inactivation of simian rotavirus SA-11 and human rotavirus type 2 (Wa) by chlorine was compared at 4 degrees C by using single-particle virus stocks. Both virus types were usually more readily inactivated at pH 6.0 than at pH 8.0 when low chlorine concentrations (0.05 to 0.2 mg/liter) were used. A complete (5 log) reduction of both was obtained within 20 s at all pH levels when chlorine concentrations were increased to 0.3 mg/liter. Slight differences in the chlorine sensitivities of SA-11 and human rotavirus type 2 were noted but were not considered to be significant. 相似文献
960.
Inhibitory Effect of Autoclaving Whey-Based Medium on Propionic Acid Production by Propionibacterium shermanii 总被引:3,自引:1,他引:2
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Thomas M. Anderson Elizabeth A. Bodie Nelson Goodman Robert D. Schwartz 《Applied microbiology》1986,51(2):427-428
Propionic acid production by Propionibacterium shermanii was compared in pasteurized and autoclaved whey-based media. Propionic acid production decreased with increasing whey concentration in autoclaved media but not in pasteurized media. Increasing the yeast extract concentration from 5 to 10 g/liter greatly reduced the inhibitory effect of autoclaving. 相似文献