全文获取类型
收费全文 | 355篇 |
免费 | 46篇 |
专业分类
401篇 |
出版年
2024年 | 1篇 |
2023年 | 6篇 |
2022年 | 6篇 |
2021年 | 7篇 |
2020年 | 14篇 |
2019年 | 8篇 |
2018年 | 7篇 |
2017年 | 8篇 |
2016年 | 26篇 |
2015年 | 22篇 |
2014年 | 26篇 |
2013年 | 31篇 |
2012年 | 31篇 |
2011年 | 33篇 |
2010年 | 14篇 |
2009年 | 14篇 |
2008年 | 28篇 |
2007年 | 10篇 |
2006年 | 18篇 |
2005年 | 17篇 |
2004年 | 19篇 |
2003年 | 15篇 |
2002年 | 10篇 |
2001年 | 4篇 |
2000年 | 2篇 |
1999年 | 3篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 1篇 |
1994年 | 2篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1990年 | 1篇 |
1987年 | 4篇 |
1982年 | 1篇 |
1976年 | 1篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1972年 | 1篇 |
1971年 | 1篇 |
1967年 | 1篇 |
排序方式: 共有401条查询结果,搜索用时 9 毫秒
41.
Stefan Krebs Ivica Medugorac Ingolf Russ Pete Ossent Ulrich Bleul Wolfgang Schmahl Martin Förster 《Mammalian genome》2006,17(1):67-76
Bovine spinal muscular atrophy (SMA), an autosomal recessive neurodegenerative disease, has been mapped at moderate resolution
to the distal part of Chromosome 24. In this article we confirm this location and fine-map the SMA locus to an interval of
approximately 0.8 cM at the very distal end of BTA24. Despite remarkable similarity to human SMA, the causative gene SMN can
be excluded in bovine SMA. However, the interval where the disease now has been mapped contains BCL2, like SMN an antiapoptotic
factor, and shown to bind to SMN. Moreover, knockout mice lacking the BCL2 gene show rapid motor neuron degeneration with
early postnatal onset, as observed in bovine SMA. A comparative cattle/human map of the distal end of BTA24, based on the
emerging bovine genome sequencing data, shows conserved synteny to HSA18 with hints of a segmental duplication and pericentic
inversion just after the last available bovine marker DIK4971. This synteny lets us conclude that SMA is in immediate vicinity of the telomere. Candidate gene analysis of BCL2, however,
excludes most of this gene, except its promoter region, and draws attention to the neighboring gene VPS4B, part of the endosomal
protein-sorting machinery ESCRT-III which is involved in several neurodegenerative diseases.
Stefan Krebs and Ivica Medugorac contributed equally to this work and agreed to be considered as first authors. 相似文献
42.
Kogut MH Swaggerty C He H Pevzner I Kaiser P 《Microbes and infection / Institut Pasteur》2006,8(7):1866-1874
43.
While banding ferruginous pygmy-owls (Glaucidium brasilianum) and Eastern screech-owls (Megascops asio) in south Texas during 2004, we recorded Philornis mimicola (Diptera: Muscidae) and Ornithodoros concanensis (Acari: Argasidae) parasitizing nestlings. Inspection of nestlings revealed 54 P. mimicola and one O. concanensis. Inspection of nest material revealed 111 P. mimicola, including 57 puparia. The effect (e.g., blood loss, anemia) of these hematophagous parasites might have contributed to the demise of at least one Eastern screech-owl nestling. This is the first record of P. mimicola and O. concanensis parasitizing ferruginous pygmy-owls and Eastern screech-owls. 相似文献
44.
Hossain MG Bharati P Aik S Lestrel PE Abeer A Kamarul T 《Journal of biosocial science》2012,44(4):385-399
Body mass index (BMI) is a good indicator of nutritional status in a population. In underdeveloped countries like Bangladesh, this indicator provides a method that can assist intervention to help eradicate many preventable diseases. This study aimed to report on changes in the BMI of married Bangladeshi women who were born in the past three decades and its association with socio-demographic factors. Data for 10,115 married and currently non-pregnant Bangladeshi women were extracted from the 2007 Bangladesh Demographic and Health Survey (BDHS). The age range of the sample was 15-49 years. The mean BMI was 20.85 ± 3.66 kg/m(2), and a decreasing tendency in BMI was found among birth year cohorts from 1972 to 1992. It was found that the proportion of underweight females has been increasing in those born during the last 20 years of the study period (1972 to 1992). Body mass index increased with increasing age, education level of the woman and her husband, wealth index, age at first marriage and age at first delivery, and decreased with increasing number of ever-born children. Lower BMI was especially pronounced among women who were living in rural areas, non-Muslims, employed women, women not living with their husbands (separated) or those who had delivered at home or non-Caesarean delivery. 相似文献
45.
46.
Kundu S Faulkes CG Greenwood AG Jones CG Kaiser P Lyne OD Black SA Chowrimootoo A Groombridge JJ 《Journal of virology》2012,86(9):5221-5229
Circoviruses are among the smallest and simplest of all viruses, but they are relatively poorly characterized. Here, we intensively sampled two sympatric parrot populations from Mauritius over a period of 11 years and screened for the circovirus Beak and feather disease virus (BFDV). During the sampling period, a severe outbreak of psittacine beak and feather disease, which is caused by BFDV, occurred in Echo parakeets. Consequently, this data set presents an ideal system for studying the evolution of a pathogen in a natural population and to understand the adaptive changes that cause outbreaks. Unexpectedly, we discovered that the outbreak was most likely caused by changes in functionally important regions of the normally conserved replication-associated protein gene and not the immunogenic capsid. Moreover, these mutations were completely fixed in the Echo parakeet host population very shortly after the outbreak. Several capsid alleles were linked to the replication-associated protein outbreak allele, suggesting that whereas the key changes occurred in the latter, the scope of the outbreak and the selective sweep may have been influenced by positive selection in the capsid. We found evidence for viral transmission between the two host populations though evidence for the invasive species as the source of the outbreak was equivocal. Finally, the high evolutionary rate that we estimated shows how rapidly new variation can arise in BFDV and is consistent with recent results from other small single-stranded DNA viruses. 相似文献
47.
Nadine S. Lossi Eleni Manoli Pete Simpson Cerith Jones Kailyn Hui Sarah J. Coulthurst Paul Freemont Alain Filloux 《Molecular microbiology》2012,86(2):437-456
In Pseudomonas aeruginosa three type VI secretion systems (T6SSs) coexist, called H1‐ to H3‐T6SSs. Several T6SS components are proposed to be part of a macromolecular complex resembling the bacteriophage tail. The T6SS protein HsiE1 (TagJ) is unique to the H1‐T6SS and absent from the H2‐ and H3‐T6SSs. We demonstrate that HsiE1 interacts with a predicted N‐terminal α‐helix in HsiB1 (TssB) thus forming a novel subcomplex of the T6SS. HsiB1 is homologous to the Vibrio cholerae VipA component, which contributes to the formation of a bacteriophage tail sheath‐like structure. We show that the interaction between HsiE1 and HsiB1 is specific and does not occur between HsiE1 and HsiB2. Proteins of the TssB family encoded in T6SS clusters lacking a gene encoding a TagJ‐like component are often devoid of the predicted N‐terminal helical region, which suggests co‐evolution. We observe that a synthetic peptide corresponding to the N‐terminal 20 amino acids of HsiB1 interacts with purified HsiE1 protein. This interaction is a common feature to other bacterial T6SSs that display a TagJ homologue as shown here with Serratia marcescens. We further show that hsiE1 is a non‐essential gene for the T6SS and suggest that HsiE1 may modulate incorporation of HsiB1 into the T6SS. 相似文献
48.
Pete Smith 《Global Change Biology》2012,18(1):35-43
Agricultural lands occupy about 40–50% of the Earth's land surface. Agricultural practices can make a significant contribution at low cost to increasing soil carbon sinks, reducing greenhouse gas (GHG) emissions and contributing biomass feedstocks for energy use. Considering all gases, the global technical mitigation potential from agriculture (excluding fossil fuel offsets from biomass) by 2030 is estimated to be ca. 5500–6000 Mt CO2‐eq. yr?1. Economic potentials are estimated to be 1500–1600, 2500–2700 and 4000–4300 Mt CO2‐eq. yr?1 at carbon prices of up to $US20, 50 and 100 t CO2‐eq.?1, respectively. The value of the global agricultural GHG mitigation at the same three carbon prices is $US32 000, 130 000 and 420 000 million yr?1, respectively. At the European level, early estimates of soil carbon sequestration potential in croplands were ca. 200 Mt CO2 yr?1, but this is a technical potential and is for geographical Europe as far east as the Urals. The economic potential is much smaller, with more recent estimates for the EU27 suggesting a maximum potential of ca. 20 Mt CO2‐eq. yr?1. The UK is small in global terms, but a large part of its land area (11 Mha) is used for agriculture. Agriculture accounts for about 7% of total UK GHG emissions. The mitigation potential of UK agriculture is estimated to be ca. 1–2 Mt CO2‐eq. yr?1, accounting for less than 1% of UK total GHG emissions. 相似文献
49.
50.