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91.
Intracellular localization of enzymes in yeast 总被引:18,自引:0,他引:18
92.
93.
Michael E. Perlman 《Nucleosides, nucleotides & nucleic acids》2013,32(1):73-82
Abstract The 2′-deuterio arabino analogs of tubercidin and adenosine have been prepared by Swern oxidation of the 3′,5′-TPDS derivatives of tubercidin and adenosine and reduction with NaBD4. Subsequent inversion of stereochemistry at C-2′ yielded [2′-2H]tubercidin and [2′-2H]adenosine with 98% deuterium incorporation. 相似文献
94.
Basal body addition in ciliary rows of Tetrahymena pyriformis 总被引:1,自引:0,他引:1
B S Perlman 《The Journal of experimental zoology》1973,184(3):365-368
95.
Seetharamaiyer Padmanabhan Ruth C. Lavin Paresh M. Thakker Jinqing Guo Lu Zhang Deke Moore Michael E. Perlman Cassandra Kirk Deborah Daly Kathy J. Burke-Howie Teresa Wolcott Suchitra Chari David Berlove James B. Fischer William F. Holt Graham J. Durant Robert N. McBurney 《Bioorganic & medicinal chemistry letters》2001,11(24):1945-3155
Solution-phase synthesis of various acylguanidine derivatives and the evaluation of a small library of compounds as potential sodium channel blockers are described. 相似文献
96.
J H Perlman D R Nussenzveig R Osman M C Gershengorn 《The Journal of biological chemistry》1992,267(34):24413-24417
Thyrotropin-releasing hormone, TRH (< Glu-His-Proamide), and [N tau-Me-His]TRH (MeTRH) are present as neutral and positively charged forms at physiologic pH, and it was possible that they bind to the TRH receptor (TRH-R) as charged (protonated) species. Binding affinities of TRH and MeTRH to endogenous rat TRH-Rs and to transfected wild type mouse TRH-Rs decreased below pH 7.1. Half-maximal decreases in binding occurred at the approximate pK alpha values of these ligands. Asp to Ala mutations in extracellular loop 1, TM-4, and TM-5 did not decrease binding affinity, but an Asp to Ala mutation in TM-2 caused the affinity to decrease 8-fold. The pH dependences of binding of MeTRH, however, were similar in wild type and all mutant receptors and were consistent with the protonated form of MeTRH binding less well. Thus, the binding of TRH to its receptor does not involve ionic interactions and may be a prototype for binding of neutral peptide ligands to G protein-coupled receptors. 相似文献
97.
Drosophila recens is parasitized in the wild by two nematodes, Howardula aoronymphium , a host generalist, and Parasitylenchus nearcticus , a host specialist known only from D . recens . In order to understand how these two parasite species coexist, we compared their ability to infect and grow in D . recens , their effects on host fecundity and survival, and whether one parasite species was competitively superior in double infections. The specialist nematode P. nearcticus had greater rates of infection and reproduction than the generalist H. aoronymphium , and completely sterilized females in single and mixed infections. The specialist was competitively superior in mixed infections, as generalist motherworms were significantly smaller than in single infections. These results suggest that P. nearcticus might competitively exclude H. aoronymphium if D. recens were the only host available. It is likely that H. aoronymphium persists in D. recens by transmission from other, more suitable host species. 相似文献
98.
The fermentation of L-sorbose by Gluconobacter melanogenus. I. General characteristics of the fermentation 总被引:2,自引:0,他引:2
Growing cultures, washed cells, and cell-free preparations of Gluconobacter melanogecnus IFO 3293 converted L -sorbose to 2-keto-L -gulonic acid, to D -sorbitol (which was metabolized further) and to 5-keto-D -fructose. 相似文献
99.
We have performed a detailed analysis of the properties of glucose-repressed cells of a commercial strain of Saccharomyces cerevisiae. They contain measurable amounts of the respiratory enzymes NADH oxidase, cytochrome c oxidase, succinate dehydrogenase, succinate:cytochrome c reductase and NADH:cytochrome c reductase (antimycin A-sensitive) as well as the dehydrogenases for l-malate, l-glutamate, and l8-isocitrate. Cytochromes b, c1, and aa3 are present in amounts that may be in excess of those required for cytochrome-linked enzyme activities. Enzymes and cytochromes are localized in large, presumably mitochondrial organelles among which no compositional or functional heterogeneity could be detected.We have also analyzed the kinetics of synthesis of respiratory enzymes and cytochromes during the release from catabolite(glucose) repression. All activities assayed except for cytochrome c oxidase begin their derepression before the external glucose concentration falls below 0.4%; derepression of cytochrome oxidase occurs only after the glucose concentration falls below 0.1%. The earlier events comprise the “fermentative” phase of derepression while the later events comprise the “oxidative” phase. The two phases can be distinguished operationally by their sensitivity to antimycin A. Only the oxidative phase is blocked by the inhibitor. Respiratory enzymes and cytochromes appear to fall into two classes distinguishable by their increase during derepression. An apparently constitutive one consists of cytochrome c oxidase, ATPase, and cytochromes aa3, b, and c1; these entities increase in amount per cell but not in amount per unit of mitochondrial mass and are of the order of 5-fold or less. The second class consists of those activities that increase by more than 6-fold and may be considered derepressible in the strict sense. Thus, proliferation and differentiation of mitochondria both contribute to the cellular changes associated with derepression.The fermentative phase of derepression does not require mitochondrial function, mitochondrial protein, or RNA synthesis, or the gradual accumulation of regulatory elements for either its initiation or persistence. This phase of derepression also occurs in cytoplasmic petites. In contrast, the oxidative phase of derepression requires mitochondrial function. Mitochondrial gene expression is required for the biogenesis of fully functional mitochondria but, except for cytochrome c, it plays little or no role in regulating the expression of nuclear genes the products of which are localized in mitochondria. 相似文献