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961.
V.P. da Cunha T.M. Preisser M.P. Santana D.C.C. Machado V.B. Pereira A. Miyoshi 《Journal of applied microbiology》2020,129(5):1389-1401
962.
Raquel Tatiane Pereira Caroline Nebo Luciana de Paula Naves Rodrigo Fortes-Silva Izabela Regina Cardoso de Oliveira Renan Rosa Paulino Cristina Delarete Drummond Priscila Vieira Rosa 《Journal of morphology》2020,281(1):55-67
Goblet cells (GCs) and endocrine cells (ECs) play an important role in intestine physiology, and few studies currently exist for Amazonian fishes. This study aimed to quantify the distribution of GCs and ECs producing cholecystokinin-8 and neuropeptide Y, assessed by mucin histochemistry and peptides immunohistochemistry, in the intestine of two Amazonian species with different feeding habits Tambaqui (Colossosoma macropomum) and hybrid catfish (Pseudoplatystoma reticulatum × Leiarius marmoratus), an omnivore and carnivore, respectively. A systematic literature review correlating feeding habit and GC and EC distribution was also included to contribute to the comparative study. The results of this study provided novel information about the gut cells of Tambaqui and hybrid catfish. Both, GCs and ECs can be found sweeping the entire intestine of Tambaqui and hybrid catfish although the cells can be more concentrated in certain segments. The GCs and ECs in Tambaqui were more uniformly distributed in the midgut segments (T1, T2, and T3). Unlike, in hybrid catfish GCs were more concentrated in the hindgut (C4) and ECs mainly in the two midgut segments (C1 and C2) of hybrid catfish. Based on the comparison between Tambaqui, hybrid catfish, and other fishes in the literature review, we suggest that cell distribution can be partially explained by feeding habits, carnivorous vs. omnivorous. 相似文献
963.
Crislei Larentis Mara Cristina Baldasso Bruna Caroline Kotz Kliemann Mayara Pereira Neves Arielli Giachini Zavaski Leticia Mazzuco Sandri Ana Cristina Ribeiro Daniel Pereira de Sousa Simes Xavier Giovanni de Oliveira Nagasawa Costa Rosilene Luciana Delariva 《Zeitschrift fur angewandte Ichthyologie》2019,35(5):1164-1168
964.
Maria J. Pena Susana G. Guerreiro Júlio C. Rocha Tiago Morais Sofia S. Pereira Mariana P. Monteiro Nuno Borges 《Journal of cellular biochemistry》2019,120(8):13056-13065
Amino acid mixtures (AAM) are protein substitutes used for phenylketonuria treatment, but their metabolic effects have not been well characterized. The objective of this study was to compare the acute glycemic response to free amino acids (free AA) from AAM with the response to intact protein (iProtein). Male Wistar rats (n = 14) were administered by gavage a bolus of free AA (n = 7) or iProtein as albumin (n = 7) containing equivalent amounts of nitrogen. Blood glucose and insulin levels were measured at baseline and 15, 30, 60 and 120 minutes later, when gut GLP-1 content and pancreatic insulin, GLP-1 receptor and Ki67 expression were quantified at 120 minutes time point. After AAM, glucose area under the curve (free AA vs iProtein; P < 0.01), serum insulin levels at 120 minutes (free AA vs iProtein; P < 0.05), colon GLP-1 content (free AA vs iProtein; P < 0.01), pancreatic GLP-1 receptor (free AA vs iProtein; P < 0.01) and insulin expression (free AA vs iProtein; p < 0.01) were significantly lower as compared with iProtein. AAM increased Ki67 expression in pancreatic islets (free AA vs iProtein; P < 0.05). In conclusion, this study demonstrated that acute response to AAM differs from iProtein and is characterized by a lower glucose excursion, along with a decrease in gut GLP-1 and pancreatic GLP-1 receptor and insulin. This data suggests the modulation of glycemia by free AA is mediated by the incretin axis. 相似文献
965.
Dahyana Santos Britto Carlos Priminho Pirovani Bruno Silva Andrade Tassiara Pereira dos Santos Cristina Pungartnik Júlio Cezar M. Cascardo Fabienne Micheli Abelmon S. Gesteira 《Molecular biology reports》2013,40(9):5417-5427
In this work, we identified a gene from Theobroma cacao L. genome and cDNA libraries, named TcGlu2, that encodes a β-1,3-1,4-glucanase. The TcGlu2 ORF was 720 bp in length and encoded a polypeptide of 239 amino acids with a molecular mass of 25.58 kDa. TcGlu2 contains a conserved domain characteristic of β-1,3-1,4-glucanases and presented high protein identity with β-1,3-1,4-glucanases from other plant species. Molecular modeling of TcGlu2 showed an active site of 13 amino acids typical of glucanase with β-1,3 and 1,4 action mode. The recombinant cDNA TcGlu2 obtained by heterologous expression in Escherichia coli and whose sequence was confirmed by mass spectrometry, has a molecular mass of about 22 kDa (with His-Tag) and showed antifungal activity against the fungus Moniliophthora perniciosa, causal agent of the witches’ broom disease in cacao. The integrity of the hyphae membranes of M. perniciosa, incubated with protein TcGlu2, was analyzed with propidium iodide. After 1 h of incubation, a strong fluorescence emitted by the hyphae indicating the hydrolysis of the membrane by TcGlu2, was observed. To our knowledge, this is the first study of a cacao β-1,3-1,4-glucanase expression in heterologous system and the first analysis showing the antifungal activity of a β-1,3-1,4-glucanase, in particular against M. perniciosa. 相似文献
966.
Lucas Pereira Martins Anderson Matos Medina Thomas M. Lewinsohn Mário Almeida-Neto 《Biotropica》2020,52(4):664-674
The structural organization of several antagonistic networks has been demonstrated to be largely conserved through time and space even when species beta-diversity is high. This might occur either because species are replaced by others that fulfill similar network roles or because interaction probabilities are given by species relative abundances rather than by their functional traits. Alternatively, if species-specific traits are important drivers of realized interactions, any change in species composition should promote a certain degree of network structural dissimilarity. Here, we used a spatial-temporal system comprising asteraceous plants and flower head herbivores from remnants of Brazilian Cerrado to investigate whether the relationship between spatial beta-diversity of species and network structural dissimilarity changes over time. We measured species beta-diversity using Sørensen's dissimilarity index (βsor) and its components of species replacement (β-3) and richness differences (βrich). Network structural dissimilarity was estimated using three different metrics: connectance, modularity, and web asymmetry. We show that, in general, the effect of species beta-diversity on network structure was time-dependent: While some periods presented a positive relationship between spatial beta-diversity and network structural dissimilarity, others presented no significant relationship. This indicates that functionally similar species may present different turnover rates at distinct periods, and different non-exclusive processes affect plant–herbivore network organization across time. 相似文献
967.
968.
Inês Correia Rosa Joana Luísa Pereira Raquel Costa Fernando Gon?alves Robert Prezant 《PloS one》2012,7(10)
Temperature is a determinant environmental variable in metabolic rates of organisms ultimately influencing important physiological and behavioural features. Stressful conditions such as increasing temperature, particularly within high ranges occurring in the summer, have been suggested to induce flotation behaviour in Corbicula fluminea which may be important in dispersal of this invasive species. However, there has been no experimental evidence supporting this hypothesis. It was already proven that C. fluminea drift is supported by a mucilaginous drogue line produced by mucocytes present in the ctenidia. Detailed microscopic examination of changes in these cells and quantification of clam flotation following one, two and three weeks of exposure to 22, 25 and 30°C was carried out so that the effects of increasing water temperatures in dispersal patterns could be discussed. Results show that changes in temperature triggered an acceleration of the mucocytes production and stimulated flotation behaviour, especially following one week of exposure. Dilution of these effects occurred following longer exposure periods. It is possible that these bivalves perceive changing temperature as a stress and respond accordingly in the short-term, and then acclimate to the new environmental conditions. The response patterns suggest that increasing water temperatures could stimulate C. fluminea population expansion. 相似文献
969.
M J Avila-Campos N L Raymundo L M Farias S L Silva M A Carvalho C A Damasceno L H Pereira E O Cisalpino 《Laboratory animals》1990,24(1):68-70
Thirty-five strains of the Bacteroides fragilis group were isolated from oral and intestinal samples from 5 wild caught, captive Callithrix penicillata. Nine oral strains of Bacteroides fragilis (7) and Bacteroides distasonis (2), and 26 intestinal strains of Bacteroides fragilis (14) and Bacteroides distasonis (12) were identified. 相似文献
970.
Anatomy of the herpes simplex virus 1 strain F glycoprotein B gene: primary sequence and predicted protein structure of the wild type and of monoclonal antibody-resistant mutants. 总被引:58,自引:49,他引:9
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In this paper we report the nucleotide sequence and predicted amino acid sequence of glycoprotein B of herpes simplex virus 1 strain F and the amino acid substitutions in the domains of the glycoprotein B gene of three mutants selected for resistance to monoclonal antibody H126-5 or H233 but not to both. Analyses of the amino acid sequence with respect to hydropathicity and secondary structure yielded a two-dimensional model of the protein. The model predicts an N-terminal, 29-amino-acid cleavable signal sequence, a 696-amino-acid hydrophilic surface domain containing six potential sites for N-linked glycosylation, a 69-amino-acid hydrophobic domain containing three segments traversing the membrane, and a charged 109-amino-acid domain projecting into the cytoplasm and previously shown to marker rescue glycoprotein B syn mutations. The nucleotide sequence of the mutant glycoprotein B DNA fragments previously shown to marker transfer or rescue the mutations revealed that the amino acid substitutions cluster in the hydrophilic surface domain between amino acids 273 and 305. Analyses of the secondary structure of these regions, coupled with the experimentally derived observation that the H126-5- and H233-antibody cognitive sites do not overlap, indicate the approximate locations of the epitopes of these neutralizing, surface-reacting, and immune-precipitating monoclonal antibodies. The predicted perturbations in the secondary structure introduced by the amino acid substitutions correlate with the extent of loss of reactivity with monoclonal antibodies in various immunoassays. 相似文献