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31.
An automated procedure has been described based on the manual method of Hagen and Hagen [(1962) Canad. J. Biochem.40, 1129] which will rapidly and reproducibly measure glycerol concentrations. The method was developed primarily for the determination of glycerol in adipose tissue and various incubation media. The glycerol in solution is estimated by its partial conversion to dihydroxyacetone by glycerol dehydrogenase from Aerobacter aerogenes. The range of glycerol concentrations able to be measured is more extensive than with other automated methods. 相似文献
32.
A monoclonal antibody that identifies a membrane molecule unique in rat lung for type II alveolar epithelial cells was used to isolate these cells from enzymatically dispersed lung cells by fluorescence-activated cell sorting. Although multistep physical separation techniques have permitted the isolation of large quantities of these cells and flow cytometry has been used by others to isolate lamellar body-containing cells, the application of this antibody-directed sorting has distinct advantages. Because the marker molecule is expressed on immature type II cells prior to the development of lamellar bodies, the antibody will also permit their isolation and study. 相似文献
33.
Organic matter contributed to soil by plant roots during the growth and decomposition of maize 总被引:1,自引:0,他引:1
Maize (Zea mays var. Caldera) plants were grown under sterile and not sterile conditions in soil in an atmosphere continuously enriched with 14CO2 for 36 days. At harvest the above ground parts of the maize were cut off and the roots were separated from the soil by washing with water. The soil was dispersed using ultrasonics and separated into soluble clay silt and sand fraction. Roots were included in the coarse sand fraction. 25% of the total label present in the soil 5.5% of that in the soil-plant system, was water soluble. Very little label was present in the clay and silt fractions (5% in each) and most (65%) was in the sand fraction as root material.Rapid extraction of soil after the removal of roots without ultrasonic treatment released soluble matter which amounted to <0.5% of the total activity in the soil-plant system.Isolated roots steeped in water released about 18% of their activity. Much of the soluble fraction may therefore be root lysate.The soil and roots accounted for 22% of the total activity in the soil-plant system. Glucose accounted for 89% of the sugars in the soluble fraction of the soil.78% or more of the 14C present in glucose, arabinose and xylose constituents of the root-soil mixture occurred in the coarse and fine sand fractions, which also included root material. For mannose and galactose the value was 70% and for rhamnose, 50%.After reinoculation of the soil-root mixture and decomposition for 56 weeks, the water soluble material obtained on fractionation of the soil decreased to less than 1% of the total activity. A much greater proportion, 25%, was present in the clay fraction as a result of decomposition. 相似文献
34.
35.
The roles of cell replication and shape change as morphogenetic forces in epithelial invagination were examined in instar II Artemia. The epidermal cells underwent a fixed pattern of cell division during the first 5 hr of instar II. Greater cell replication in the thoracopod bud (ThB) than in the arthrodial membrane (AM) region resulted in a higher density of epidermal cells in the ThB region (differential cell density). The ratio of cell density (AM/ThB) declined from 1.0 to less than 0.80 by Hour 2 of instar II. Invagination of the AM occurred during Hour 4 when the AM/ThB reached 0.75. A 2-hr pulse with 5'-fluorodeoxyuridine (FudR) during instar I delayed completion of the cell replication pattern and development of transverse cell files in the ThB region for a period equal to the length of the exposure. The delay in the cell division program resulted in a cell density ratio of 0.93 at Hour 4, a value normally observed in Hour 2 larvae, and evagination of the epidermis did not occur at apolysis (Hour 4). The FudR treatment did not perturb the cytoskeleton or the initial steps in cell shape change and the larvae formed small segments during instar III. Cell shape change within the AM began during Hour 4 as this region became significantly thinner than the neighboring ThB region (thickness ratio, AM/ThB = 0.77). Before apolysis the AM cells became wedge shaped, a change which occurred when the basal region of the cell enlarged. The microtubules and microfilaments were reorganized from the apical cytoplasm to the lateral border of apposing AM cells. Following apolysis (Late Hour 4) shape change was completed as the cells attained a thin spindle form, with microtubule- and microfilament-rich filopodial extensions which overlapped adjacent AM cells. As contact with ThB cells shifted from lateral to apicolateral, the AM cells formed the innermost edge of the invagination. Microtubules in the differentiating AM cells contained tyrosinated, detyrosinated, and acetylated alpha-tubulin isoforms. Treatment with nocodazole, colchicine, taxol, or cytochalasin B blocked AM cell shape change and inhibited segmentation, but did not affect the mitotic pattern or differential cell density. We conclude that the specific pattern of cell division led to differential cell density which, along with AM cell shape change, established the conditions necessary to achieve epidermal evagination. 相似文献
36.
Hamish Gibbs Emily Nightingale Yang Liu James Cheshire Leon Danon Liam Smeeth Carl A. B. Pearson Chris Grundy LSHTM CMMID COVID- working group Adam J. Kucharski Rosalind M. Eggo 《PLoS computational biology》2021,17(7)
On March 23 2020, the UK enacted an intensive, nationwide lockdown to mitigate transmission of COVID-19. As restrictions began to ease, more localized interventions were used to target resurgences in transmission. Understanding the spatial scale of networks of human interaction, and how these networks change over time, is critical to targeting interventions at the most at-risk areas without unnecessarily restricting areas at low risk of resurgence. We use detailed human mobility data aggregated from Facebook users to determine how the spatially-explicit network of movements changed before and during the lockdown period, in response to the easing of restrictions, and to the introduction of locally-targeted interventions. We also apply community detection techniques to the weighted, directed network of movements to identify geographically-explicit movement communities and measure the evolution of these community structures through time. We found that the mobility network became more sparse and the number of mobility communities decreased under the national lockdown, a change that disproportionately affected long distance connections central to the mobility network. We also found that the community structure of areas in which locally-targeted interventions were implemented following epidemic resurgence did not show reorganization of community structure but did show small decreases in indicators of travel outside of local areas. We propose that communities detected using Facebook or other mobility data be used to assess the impact of spatially-targeted restrictions and may inform policymakers about the spatial extent of human movement patterns in the UK. These data are available in near real-time, allowing quantification of changes in the distribution of the population across the UK, as well as changes in travel patterns to inform our understanding of the impact of geographically-targeted interventions. 相似文献
37.
Seo ES Blaum BS Vargues T De Cecco M Deakin JA Lyon M Barran PE Campopiano DJ Uhrín D 《Biochemistry》2010,49(49):10486-10495
Human β-defensin 2 (HBD2) is a member of the defensin family of antimicrobial peptides that plays important roles in the innate and adaptive immune system of both vertebrates and invertebrates. In addition to their direct bactericidal action, defensins are also involved in chemotaxis and Toll-like receptor activation. In analogy to chemokine/glycosaminoglycan (GAG) interactions, GAG-defensin complexes are likely to play an important role in chemotaxis and in presenting defensins to their receptors. Using a gel mobility shift assay, we found that HBD2 bound to a range of GAGs including heparin/heparan sulfate (HS), dermatan sulfate (DS), and chondroitin sulfate. We used NMR spectroscopy of (15)N-labeled HBD2 to map the binding sites for two GAG model compounds, a heparin/HS pentasaccharide (fondaparinux sodium; FX) and enzymatically prepared DS hexasaccharide (DSdp6). We identified a number of basic amino acids that form a common ligand binding site, which indicated that these interactions are predominantly electrostatic. The dissociation constant of the [DSdp6-HBD2] complex was determined by NMR spectroscopy to be 5 ± 5 μM. Binding of FX could not be quantified because of slow exchange on the NMR chemical shift time scale. FX was found to induce HBD2 dimerization as evidenced by the analysis of diffusion coefficients, (15)N relaxation, and nESI-MS measurements. The formation of FX-bridged HBD2 dimers exhibited features of a cooperative binding mechanism. In contrast, the complex with DSdp6 was found to be mostly monomeric. 相似文献
38.
Bernhardt A Lechner E Hano P Schade V Dieterle M Anders M Dubin MJ Benvenuto G Bowler C Genschik P Hellmann H 《The Plant journal : for cell and molecular biology》2006,47(4):591-603
Cullins are central scaffolding subunits in eukaryotic E3 ligases that facilitate the ubiquitination of target proteins. Arabidopsis contains at least 11 cullin proteins but only a few of them have been assigned biological roles. In this work Arabidopsis cullin 4 is shown to assemble with DDB1, RBX1, DET1 and DDB2 in vitro and in planta. In addition, by using T-DNA insertion and CUL4 antisense lines we demonstrate that corresponding mutants are severely affected in different aspects of development. Reduced CUL4 expression leads to a reduced number of lateral roots, and to abnormal vascular tissue and stomatal development. Furthermore, cul4 mutants display a weak constitutive photomorphogenic phenotype. These results therefore assign an important function to CUL4 during plant development and provide strong evidence that CUL4 assembles together with RBX1 and DDB1 proteins to form a functional E3 ligase in Arabidopsis. 相似文献
39.
Guile SD Bantick JR Cheshire DR Cooper ME Davis AM Donald DK Evans R Eyssade C Ferguson DD Hill S Hutchinson R Ingall AH Kingston LP Martin I Martin BP Mohammed RT Murray C Perry MW Reynolds RH Thorne PV Wilkinson DJ Withnall J 《Bioorganic & medicinal chemistry letters》2006,16(8):2260-2265
A novel series of potent blockers of the monocarboxylate transporter, MCT1, is disclosed. From very potent but lipophilic lead compounds, systematic changes to all parts of the molecule, targeting reduction in log D, afforded compounds with significantly improved overall properties. These compounds show potent immunomodulatory activity. 相似文献
40.
J. C. Stahl J. A. Bartle N. G. Cheshire C. Petyt P. M. Sagar 《New Zealand journal of zoology.》2013,40(2):109-137
Abstract The distribution and movements of Buller's albatross in Australasian seas are analysed using results of shipborne surveys (13 238 10‐min counts), counts from trawlers, banding data, recoveries on beaches and fishing vessels, and records from the literature. Patterns of marine distribution are documented by monthly accounts and maps. During the breeding season, highest abundances are recorded over shelves and slopes off southern New Zealand (The Snares shelf to 41–43°S off the South Island, D. b. bulleri), around the Chatham Islands and over oceanic subtropical waters east of New Zealand (probably D. b. platei), with marked seasonal variations observed off southern New Zealand. Both subspecies disperse mostly outside Australasian waters during the non‐breeding season. Birds banded on The Snares were recovered off south‐eastern New Zealand (Stewart Island to Cook Strait) and in the eastern tropical Pacific. Immatures accounted for only 0.25% of birds censused during the ship‐borne surveys; they are recorded around the New Zealand mainland in August‐October and February‐May, off south‐eastern Australia and in the Tasman Sea in November‐December, February, and June‐July. Around New Zealand, males predominate among birds recovered along the eastern seaboard, whereas the sex ratio in south‐western waters tends to vary according to water depth and season. Distribution patterns and movements in New Zealand and Australian seas are discussed in relation to breeding events and breeding status. 相似文献