Methanogenic Fermentation of Benzoate

A methanogenic enrichment culture decomposed small concentrations of (14)C-benzoate to (14)C(4) and (14)CO(2) under stringently anaerobic conditions with or without preceding exposure to benzoate.     

Effect of slide projections and tape-recorded suggestions on thermal biofeedback training

A previous experiment (Herzfeld & Taub, 1977) demonstrated that slide projections and explicit suggestions relating to thermal experiences can significantly augment temperature self-regulations training (.5 degrees F, p less than .05). however, the experimental design was thought to result in an underestimate of the magnitude of the effect, since each subject served as his own control and could therefore make use on nonsuggestion training days of material provided on training days. In this experiment, separate groups of subjects either were given feedback combined with the suggestion procedure on each of 8 training days or were given feedback alone. The mean temperature self-regulation of the suggestion group (2.01 degrees F) was significantly better than the self-regulation (.73) of the nonsuggestion group (t test, p less than 0.01), a difference of 1.28 degrees F.     

Platinum(II) binding to metallothioneins

The reaction of equine renal metallothionein (MT) with excess K2PtCl4 at pH 2 results in a polymeric adduct containing 17 +/- 2 mol Pt/mol MT. A monomeric adduct containing 7 mol Pt/mol MT is obtained at neutral pH. Rates of reaction of Pt7MT with DTNB and iodoacetic acid are consistent with Pt2+ to cysteine thiolate coordination, and the extent of reaction in both cases is 11 +/- 2 mol cys/mol MT. Adducts from the reaction of K2PtCl4 with apoMT chemically modified at the N-terminal methionine residue, Cd7MT, and native MT are also reported. A structural model of Pt7MT is proposed in which the square planar tetrathiolate Pt(II) unit is incorporated into a three-metal beta cluster. Implications for the metabolism of platinum anticancer drugs are discussed.