全文获取类型
收费全文 | 1954篇 |
免费 | 180篇 |
国内免费 | 210篇 |
出版年
2024年 | 7篇 |
2023年 | 48篇 |
2022年 | 100篇 |
2021年 | 104篇 |
2020年 | 98篇 |
2019年 | 127篇 |
2018年 | 102篇 |
2017年 | 76篇 |
2016年 | 112篇 |
2015年 | 109篇 |
2014年 | 134篇 |
2013年 | 141篇 |
2012年 | 187篇 |
2011年 | 179篇 |
2010年 | 101篇 |
2009年 | 91篇 |
2008年 | 97篇 |
2007年 | 74篇 |
2006年 | 59篇 |
2005年 | 40篇 |
2004年 | 52篇 |
2003年 | 33篇 |
2002年 | 30篇 |
2001年 | 20篇 |
2000年 | 33篇 |
1999年 | 21篇 |
1998年 | 17篇 |
1997年 | 10篇 |
1996年 | 10篇 |
1995年 | 21篇 |
1994年 | 12篇 |
1993年 | 8篇 |
1992年 | 12篇 |
1991年 | 8篇 |
1990年 | 10篇 |
1989年 | 12篇 |
1988年 | 4篇 |
1987年 | 7篇 |
1986年 | 5篇 |
1985年 | 5篇 |
1984年 | 5篇 |
1983年 | 5篇 |
1981年 | 3篇 |
1980年 | 2篇 |
1974年 | 1篇 |
1973年 | 2篇 |
1972年 | 2篇 |
1971年 | 1篇 |
1970年 | 3篇 |
1969年 | 1篇 |
排序方式: 共有2344条查询结果,搜索用时 15 毫秒
81.
Hang Thi Dao G. Andrew C. Beattie Amy Y. Rossman Lester W. Burgess Paul Holford 《Mycological Progress》2016,15(5):47
This study led to the discovery of four putative entomopathogenic fungi of armoured scale insects on citrus trees in coastal New South Wales. Two of these species belong in Podonectria as P. coccicola (Ellis & Everh.) Petch (syn. Tetracrium coccicola (Höhn.) Ellis & Everh.) and P. novae-zelandiae Dingley. Members of this genus are grown in culture for the first time. Formerly placed in the Pleosporales, Tubeufiaceae, or more recently in the Tubeufiales, these species are herein placed in the new family Podonectriaceae fam. nov., Pleosporales. Another species is placed in the Hypocreales, Bionectriaceae as Clonostachys coccicola (J.A. Stev.) H.T. Dao comb. nov. (basionym Tubercularia coccicola J.A. Stev., syn. Nectria tuberculariae Petch). The fourth species is Myriangium citri Henn. (Myriangiales, Myriangiaceae). Each fungal species is characterized and the phylogenetic placement confirmed by molecular analyses of the ITS and 28 s rDNA regions. In addition, their biology is noted, including location of the fungi within tree canopies. 相似文献
82.
83.
Cemal Cagatay Bilgin Gerald Fontenay Qingsu Cheng Hang Chang Ju Han Bahram Parvin 《PloS one》2016,11(3)
BioSig3D is a computational platform for high-content screening of three-dimensional (3D) cell culture models that are imaged in full 3D volume. It provides an end-to-end solution for designing high content screening assays, based on colony organization that is derived from segmentation of nuclei in each colony. BioSig3D also enables visualization of raw and processed 3D volumetric data for quality control, and integrates advanced bioinformatics analysis. The system consists of multiple computational and annotation modules that are coupled together with a strong use of controlled vocabularies to reduce ambiguities between different users. It is a web-based system that allows users to: design an experiment by defining experimental variables, upload a large set of volumetric images into the system, analyze and visualize the dataset, and either display computed indices as a heatmap, or phenotypic subtypes for heterogeneity analysis, or download computed indices for statistical analysis or integrative biology. BioSig3D has been used to profile baseline colony formations with two experiments: (i) morphogenesis of a panel of human mammary epithelial cell lines (HMEC), and (ii) heterogeneity in colony formation using an immortalized non-transformed cell line. These experiments reveal intrinsic growth properties of well-characterized cell lines that are routinely used for biological studies. BioSig3D is being released with seed datasets and video-based documentation. 相似文献
84.
Wan-Jun Chen Sheng-Jie Lai Yang Yang Kun Liu Xin-Lou Li Hong-Wu Yao Yu Li Hang Zhou Li-Ping Wang Di Mu Wen-Wu Yin Li-Qun Fang Hong-Jie Yu Wu-Chun Cao 《PLoS neglected tropical diseases》2016,10(4)
BackgroundAnthrax, a global re-emerging zoonotic disease in recent years is enzootic in mainland China. Despite its significance to the public health, spatiotemporal distributions of the disease in human and livestock and its potential driving factors remain poorly understood.Conclusions/SignificanceAnthrax in China was characterized by significant seasonality and spatial clustering. The spatial distribution of human anthrax was largely driven by livestock husbandry, human density, land cover, elevation, topsoil features and climate. Enhanced surveillance and intervention for livestock and human anthrax in the high-risk regions, particularly on the Qinghai-Tibetan Plateau, is the key to the prevention of human infections. 相似文献
85.
Mitigation of ammonia inhibition by internal dilution in high‐rate anaerobic digestion of food waste leachate and evidences of microbial community response
下载免费PDF全文
![点击此处可从《Biotechnology and bioengineering》网站下载免费的PDF全文](/ch/ext_images/free.gif)
86.
87.
Pedigree‐based analysis of derivation of genome segments of an elite rice reveals key regions during its breeding
下载免费PDF全文
![点击此处可从《Plant biotechnology journal》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Degui Zhou Wei Chen Zechuan Lin Haodong Chen Chongrong Wang Hong Li Renbo Yu Fengyun Zhang Gang Zhen Junliang Yi Kanghuo Li Yaoguang Liu William Terzaghi Xiaoyan Tang Hang He Shaochuan Zhou Xing Wang Deng 《Plant biotechnology journal》2016,14(2):638-648
Analyses of genome variations with high‐throughput assays have improved our understanding of genetic basis of crop domestication and identified the selected genome regions, but little is known about that of modern breeding, which has limited the usefulness of massive elite cultivars in further breeding. Here we deploy pedigree‐based analysis of an elite rice, Huanghuazhan, to exploit key genome regions during its breeding. The cultivars in the pedigree were resequenced with 7.6× depth on average, and 2.1 million high‐quality single nucleotide polymorphisms (SNPs) were obtained. Tracing the derivation of genome blocks with pedigree and information on SNPs revealed the chromosomal recombination during breeding, which showed that 26.22% of Huanghuazhan genome are strictly conserved key regions. These major effect regions were further supported by a QTL mapping of 260 recombinant inbred lines derived from the cross of Huanghuazhan and a very dissimilar cultivar, Shuanggui 36, and by the genome profile of eight cultivars and 36 elite lines derived from Huanghuazhan. Hitting these regions with the cloned genes revealed they include numbers of key genes, which were then applied to demonstrate how Huanghuazhan were bred after 30 years of effort and to dissect the deficiency of artificial selection. We concluded the regions are helpful to the further breeding based on this pedigree and performing breeding by design. Our study provides genetic dissection of modern rice breeding and sheds new light on how to perform genomewide breeding by design. 相似文献
88.
89.
Suppressive effect of epigallocatechin‐3‐O‐gallate on endoglin molecular regulation in myocardial fibrosis in vitro and in vivo
下载免费PDF全文
![点击此处可从《Journal of cellular and molecular medicine》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Chiu‐Mei Lin Hang Chang Bao‐Wei Wang Kou‐Gi Shyu 《Journal of cellular and molecular medicine》2016,20(11):2045-2055
Epigallocatechin‐3‐O‐gallate (EGCG), derived from green tea, has been studied extensively because of its diverse physiological and pharmacological properties. This study evaluates the protective effect of EGCG on angiotensin II (Ang II)‐induced endoglin expression in vitro and in vivo. Cardiac fibroblasts (CFs) from the thoracic aorta of adult Wistar rats were cultured and induced with Ang II. Western blotting, Northern blotting, real‐time PCR and promoter activity assay were performed. Ang II increased endoglin expression significantly as compared with control cells. The specific extracellular signal‐regulated kinase inhibitor SP600125 (JNK inhibitor), EGCG (100 μM) and c‐Jun N‐terminal kinase (JNK) siRNA attenuated endoglin proteins following Ang II induction. In addition, pre‐treated Ang II‐induced endoglin with EGCG diminished the binding activity of AP‐1 by electrophoretic mobility shift assay. Moreover, the luciferase assay results revealed that EGCG suppressed the endoglin promoter activity in Ang II‐induced CFs by AP‐1 binding. Finally, EGCG and the JNK inhibitor (SP600125) were found to have attenuated endoglin expression significantly in Ang II‐induced CFs, as determined through confocal microscopy. Following in vivo acute myocardial infarction (AMI)‐related myocardial fibrosis study, as well as immunohistochemical and confocal analyses, after treatment with endoglin siRNA and EGCG (50 mg/kg), the area of myocardial fibrosis reduced by 53.4% and 64.5% and attenuated the left ventricular end‐diastolic and systolic dimensions, and friction shortening in hemodynamic monitor. In conclusion, epigallocatechin‐3‐O‐gallate (EGCG) attenuated the endoglin expression and myocardial fibrosis by anti‐inflammatory effect in vitro and in vivo, the novel suppressive effect was mediated through JNK/AP‐1 pathway. 相似文献