酶、多肽电荷数的理论计算及误差研究

对酶的电荷数随ｐＨ变化的定量关系进行了理论推导,将推导出的酶的电荷数与ｐＨ的关系式应用于多肽等电点的计算,理论计算结果与文献实验结果完全一致,并推导出酸性氨基酸、碱性氨基酸及中性氨基酸等电点的计算式,与现有计算式完全一致．应用荧光光谱和荧光偏振对肌酸激酶带电性随ｐＨ值的变化关系的研究表明,理论计算符合实验结果,表明：此文理论关系式是可靠的．     

耐热性碱性磷酸酯酶作为非放射性标记物的研究

通过生物素与亲和素－酶复合物系统或地高辛与抗地高辛－酶复合物系统可把酶间接标记到探针上．Ｒｅｎｚ等通过不同的化学方法直接把酶标记到探针上［１～３］．耐热性碱性磷酸酯酶ＦＤ－ＴＡＰ（ｔｈｅｒｍｏｓｔａｂｌｅａｌｋａｌｉｎｅｐｈｏｓｐｈａｔａｓｅ）具有耐...     

Biophysical meanings of orientation and deformation of RBCs in shear flow field of low viscosity with new Ektacytometry

ＥｋｔａｃｙｔｏｍｅｔｒｙｍｅａｓｕｒｉｎｇＲＢＣｄｅｆｏｒｍａｂｉｌｉｔｙｗａｓｆｉｒｓｔｄｅｖｅｌｏｐｅｄｂｙＢｅｓｓｉｓｅｔａｌ.ｉｎ1975[1].Ｔｈｉｓｔｅｃｈｎｉｑｕｅｈａｓｂｅｅｎｗｏｒｌｄｗｉｄｅａｃｃｅｐｔｅｄｎｏｗ.Ｈｏｗｅｖｅｒ,ｔｈｅｍｅａｎｉｎｇｏｆｄｅｆｏｒｍａｔｉｏｎｉｎｄｅｘ(ＤＩ)ｍｅａｓｕｒｅｄｗｉｔｈｔｈｉｓｍｅｔｈｏｄｉｓｓｔｉｌｌｗｏｒｔｈｓｔｕｄｙｉｎｇ.ＴｈｅｔｒａｄｉｔｉｏｎａｌＥｋｔａｃｙｔｏｍｅｔｒｙｕｓｅｓｓｕｓｐｅｎｄｉｎｇｍｅｄｉｕｍｏｆｈｉｇｈｖｉ…     

五种珍珠菜的核型研究

本文对５种珍珠菜亚属植物进行了染色体数目及核型研究。其中瓣珍珠菜２ｎ＝２４＝１２ｍ＋８ｓｍ＋４ｓｔ（２ＳＡＴ）、黑腺珍珠菜２ｎ＝２２＝２ｍ＋４ｓｍ＋６ｓｔ＋１０ｔ、泽珍珠菜２ｎ＝２４＝１４ｍ＋６ｓｍ（２ＳＡＴ）＋４ｓｔ和小叶珍珠菜２ｎ＝４８＝３４ｍ＋１０ｓｍ＋４ｓｔ的染色体数目及核型为首次报道。中国九江产的红根草核型２ｎ＝２４＝２０ｍ＋４ｓｍ（２ＳＡＴ）与日本产的核型２ｎ＝２４＝１８ｍ（１ＳＡＴ）     

Synthesis of the Cyanine 7 labeled neutrophil-specific agents for noninvasive near infrared fluorescence imaging

A neutrophil-binding peptide, cinnamoyl-F(D)LF(D)LF (cFLFLF), was labeled with the near infrared (NIR) fluorophore, Cyanine 7 (Cy7). This construct was modified with a polyethylene glycol (PEG, M_W 3.4 kDa) moiety in order to increase its solubility and bioavailability to circulating neutrophils. A preliminary noninvasive fluorescence imaging of a mouse model of ear inflammation with the fluorescent probe is presented.     

Design and synthesis of 4-[3,5-dioxo-11-oxa-4,9-diazatricyclo[5.3.1.02,6]undec-4-yl]-2-trifluoromethyl-benzonitriles as androgen receptor antagonists

A novel series of 4-[3,5-dioxo-11-oxa-4,9-diazatricyclo[5.3.1.0^2,6]undec-4-yl]-2-trifluoromethyl-benzonitriles has been synthesized. The ability of these compounds to act as antagonists of the androgen receptor was investigated and several were found to have potent activity in vitro and in vivo.     

A fatty acyl-CoA reductase highly expressed in the head of honey bee (Apis mellifera) involves biosynthesis of a wide range of aliphatic fatty alcohols

Honey bees (Apis mellifera) are social insects which have remarkable complexity in communication pheromones. These chemical signals comprise a mixture of hydrocarbons, wax esters, fatty acids, aldehydes and alcohols. In this study, we detected several long chain aliphatic alcohols ranging from C18-C32 in honey bees and the level of these alcohols varied in each body segment. C18:0Alc and C20:0Alc are more pronounced in the head, whereas C22:0Alc to C32Alc are abundant in the abdomen. One of the cDNAs coding for a fatty acyl-CoA reductase (AmFAR1) involved in the synthesis of fatty alcohols was isolated and characterized. AmFAR1 was ubiquitously expressed in all body segments with the predominance in the head of honey bees. Heterologous expression of AmFAR1 in yeast revealed that AmFAR1 could convert a wide range of fatty acids (14:0–22:0) to their corresponding alcohols, with stearic acid 18:0 as the most preferred substrate. The substrate preference and the expression pattern of AmFAR1 were correlated with the level of total fatty alcohols in bees. Reconstitution of the wax biosynthetic pathway by heterologous expression of AmFAR1, together with Euglena wax synthase led to the high level production of medium to long chain wax monoesters in yeast.