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181.
Zhenwei Qiu Yongbing Zhang Dorothea Bedigian Xiao Li Changsui Wang Hongen Jiang 《Economic botany》2012,66(3):255-263
Sesame Utilization in China: New Archaeobotanical Evidence from Xinjiang. A cache of sesame (Sesamum indicum L.) seeds, discovered in the Thousand Buddha Grottoes at Boziklik, Turpan, China, dating to ca. 700 years before present (BP), is hard evidence of their use in China since that time. Morphological and anatomical features suggest a white sesame cultivar. The sizeable quantity unearthed implies that sesame was a valued commodity that could provision the monks and enrich the diet of ancient inhabitants as an oil source. 相似文献
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William K. K. Wu Minyi He Liang Zhao Xuegang Sun Hui Li Yong Jiang Yungao Yang Kang Peng 《Cell biochemistry and function》2012,30(4):271-278
Triptolide is a diterpenoid triepoxide derived from the traditional Chinese medical herb Tripterygium wilfordii. In the present study, we demonstrated that this phytochemical attenuated colon cancer growth in vitro and in vivo. Using a proteomic approach, we found that 14‐3‐3 epsilon, a cell cycle‐ and apoptosis‐related protein, was altered in colon cancer cells treated with triptolide. In this regard, triptolide induced cleavage and perinuclear translocation of 14‐3‐3 epsilon. Taken together, our findings suggest that triptolide may merit investigation as a potential therapeutic agent for colon cancer, and its anticancer action may be associated with alteration of 14‐3‐3 epsilon. Copyright © 2012 John Wiley & Sons, Ltd. 相似文献
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185.
Ying?Wu Rui?Wu Bangjiao?Zhang Tingting?Jiang Ning?Li Kai?Qian Bao?LiuEmail author Jian?ZhangEmail author 《Plant Growth Regulation》2012,66(2):137-143
Gardenia jasminoides Ellis is an evergreen tropical plant and favorite to gardeners throughout the world. Several studies have documented that
in vitro micropropagation can be used for clonal propagation of G. jasminoides Ellis, the efficiency remained low. In addition, no information is available on the genetic and epigenetic fidelity of the
micropropagated plants. Here, we report on a simplified protocol for high efficient micropropagation of G. jasminoides Ellis cv. “Kinberly” based on enhanced branching of shoot-tips as explants. The protocol consisted of sequential use of three
media, namely, bud-induction, elongation and root-induction. By using two molecular markers, amplified fragment length polymorphism
(AFLP) and methylation sensitive amplified polymorphism (MSAP), we analyzed the genetic and DNA methylation pattern stability
of 23 morphologically normal plants randomly taken from a sub-population (>100) of micropropagated plants originated from
a single shoot-tip. We found that of >1,000 scored AFLP bands across the 23 micropropagated plants, no incident of genetic
variation was detected. In contrast, of 750 scored MSAP bands, moderate but clear alteration in several DNA methylation patterns
occurred in the majority of the 23 micropropagated plants. The changed methylation patterns involved both CG and CHG sites
representing either hyper- or hypo-methylation, which occurred without altering the total methylation levels partly due to
concomitant hyper- and hypo-methylation alterations. Our results indicated that epigenetic instability in the form of DNA
methylation patterns can be susceptible to the in vitro micropropagation process for G. jasminoides Ellis, and needs to be taken into account in the process of large-scale commercial propagation of this plant. 相似文献
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He WQ Qiao YN Zhang CH Peng YJ Chen C Wang P Gao YQ Chen C Chen X Tao T Su XH Li CJ Kamm KE Stull JT Zhu MS 《American journal of physiology. Heart and circulatory physiology》2011,301(2):H584-H591
Vascular tone, an important determinant of systemic vascular resistance and thus blood pressure, is affected by vascular smooth muscle (VSM) contraction. Key signaling pathways for VSM contraction converge on phosphorylation of the regulatory light chain (RLC) of smooth muscle myosin. This phosphorylation is mediated by Ca(2+)/calmodulin-dependent myosin light chain kinase (MLCK) but Ca(2+)-independent kinases may also contribute, particularly in sustained contractions. Signaling through MLCK has been indirectly implicated in maintenance of basal blood pressure, whereas signaling through RhoA has been implicated in salt-induced hypertension. In this report, we analyzed mice with smooth muscle-specific knockout of MLCK. Mesenteric artery segments isolated from smooth muscle-specific MLCK knockout mice (MLCK(SMKO)) had a significantly reduced contractile response to KCl and vasoconstrictors. The kinase knockout also markedly reduced RLC phosphorylation and developed force. We suggest that MLCK and its phosphorylation of RLC are required for tonic VSM contraction. MLCK(SMKO) mice exhibit significantly lower basal blood pressure and weaker responses to vasopressors. The elevated blood pressure in salt-induced hypertension is reduced below normotensive levels after MLCK attenuation. These results suggest that MLCK is necessary for both physiological and pathological blood pressure. MLCK(SMKO) mice may be a useful model of vascular failure and hypotension. 相似文献
190.
Yang Z. T. Lu D. X. Hong E.-K. Zhang B. Y. Jiang M. C. Yang Y. J. Zhang D. J. 《Molecular Biology》2020,54(6):911-918
Molecular Biology - Brassica campestris L. is the important oil-bearing crop in China. Rapeseed cake is the main byproduct of rapeseed oil extraction. As the main active ingredient in rapeseed... 相似文献