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J P Allison M A Pellegrino S Ferrone G N Callahan R A Reisfeld 《Journal of immunology (Baltimore, Md. : 1950)》1977,118(3):1004-1009
HLA antigens of both the A and B loci were shown to be associated with the high density lipoprotein fraction of serum prepared by ultracentrifugal flotation. HLA-A9 antigens were purified 100-fold with essentially complete recovery by a simple procedure of high density lipoprotein preparation involving precipitation with polyanions and ultracentrifugal flotation. The purified lipid-associated antigen was immunogenic since it elicited the formation of cytotoxic xenoantibodies in rabbits. Serum HLA-A9 antigens were found by immunoprecipitation and gel electrophoresis to consist of a 45,000 m.w. heavy chain associated with beta2-microglobulin. The size of the HLA-lipid complex (less than 190,000 m.w.) and of the HLA-deoxycholate complex (less than 102,000 m.w.) suggests that HLA antigens are shed into plasma as a complex of a single HLA molecule and a single beta2-microglobulin chain, associated with boundary lipid. 相似文献
14.
A combination of serological (cytotoxicity, binding assay, lysostrip) and immunochemical (indirect immunoprecipitation, sequential immunoprecipitation, two-dimensional gel electrophoresis) assays have shown that the monoclonal antibody Q6/64 recognizes an antigenic determinant which is expressed by certain gene products of the A and B loci of the HLA region. The determinant identified by Q6/64 is spatially close to those which define the serological polymorphism of the HLA-A, B, C antigenic system. The results presented in this study in conjunction which those recently published by other investigators indicate that sharing of determinants among HLA-A and -B allospecificities is more frequent than originally assumed on the basis of the cross-reactivity pattern obtained with alloantisera. This conclusion is in agreement with the high degree of homology in the primary amino-acid sequence of A and B allospecificities. 相似文献
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Vito Quaranta Michele A. Pellegrino Gerald N. Callahan Soldano Ferrone 《Immunogenetics》1981,13(4):311-317
The anti-H-2 alloantiserum D-32 [(BlO.A(2R) × C3H.SW) anti-C3H] is cytolytic to human lymphocytes. Fab2 blocking assays, indirect immunoprecipitation and sequential immunoprecipitation experiments showed that the anti-H-2 alloantiserum D-32 recognizes antigenic determinants which are expressed on the heavy chain of subpopulations of HLA-A, B antigens. These determinants are different from those defining the serological polymorphism of the HLA-A, B, C system, are the same as or spatially close to those recognized by the anti-HLA-A, B monoclonal antibody Q6/64 and are expressed on rabbit, rat or guinea pig lymphocytes. 相似文献
17.
The structural relationship of histocompatibility antigens W4 and W6 with other surface markers on cultured human lymphoid cells of the B type has been investigated, utilizing the lysostrip technique. W4 and W6 antigens were found to be structurally associated with antigens coded for by theB locus of theHLA region, but independent of receptors for breakdown products of the third complement component and xenogeneic red blood cells, and of antigenic moieties reacting with antibodies present in H-2 alloantisera. 相似文献
18.
DNA sequences at the sites of three insertions of the transposable element Tn5 in the histidine operon of Salmonella 总被引:4,自引:0,他引:4
Summary The DNA sequence has been determined at the sites of three independent occurrences of the transposable element Tn5 in the hisG gene of the histidine operon. All three insertion sites are adjacent to G+C-rich stretches of DNA. In all three cases sequences at the sites of insertion show homology with a region near the ends of Tn5. 相似文献
19.
Characterization of the WIDR: a human colon carcinoma cell line. 总被引:12,自引:0,他引:12
P Noguchi R Wallace J Johnson E M Earley S O'Brien S Ferrone M A Pellegrino J Milstien C Needy W Browne J Petricciani 《In vitro》1979,15(6):401-408
We describe the establishment and characterization of WiDr, a cell line derived from a human colon carcinoma. It produces carcinoembryonic antigen in culture, and has a doubling time of 15 hr with plating efficiency of 51%. The HLA antigenic profile and the allozyme genetic signature (composed of eight gene-enzyme systems) of WiDr cells are different from those of HeLa cells. Furthermore, WiDr cells possess three marker chromosomes, again distinct from the HeLa marker chromosomes. Finally, it is highly tumorigenic in four different xenogeneic animal models. Based on these studies, WiDr represents a useful model cell line for tumor cell biology investigations. 相似文献
20.
Characterization of WiDr: A human colon carcinoma cell line 总被引:1,自引:0,他引:1
P. Noguchi R. Wallace J. Johnson E. M. Earley S. O'Brien S. Ferrone M. A. Pellegrino J. Milstien C. Needy W. Browne J. Petricciani 《In vitro cellular & developmental biology. Plant》1979,15(6):401-408
Summary We describe the establishment and characterization of WiDr, a cell line derived from a human colon carcinoma. It produces
carcinoembryonic antigen in culture, and has a doubling time of 15 hr with plating efficiency of 51%. The HLA antigenic profile
and the allozyme genetic signature (composed of eight gene-enzyme systems) of WiDr cells are different from those of HeLa
cells. Furthermore, WiDr cells possess three marker chromosomes, again distinct from the HeLa marker chromosomes. Finally,
it is highly tumorigenic in four different xenogeneic animal models. Based on these studies, WiDr represents a useful model
cell line for tumor cell biology investigations. 相似文献