全文获取类型
收费全文 | 9416篇 |
免费 | 632篇 |
国内免费 | 6篇 |
出版年
2023年 | 95篇 |
2022年 | 191篇 |
2021年 | 325篇 |
2020年 | 260篇 |
2019年 | 300篇 |
2018年 | 419篇 |
2017年 | 346篇 |
2016年 | 472篇 |
2015年 | 526篇 |
2014年 | 599篇 |
2013年 | 697篇 |
2012年 | 725篇 |
2011年 | 706篇 |
2010年 | 438篇 |
2009年 | 374篇 |
2008年 | 378篇 |
2007年 | 382篇 |
2006年 | 350篇 |
2005年 | 288篇 |
2004年 | 263篇 |
2003年 | 222篇 |
2002年 | 201篇 |
2001年 | 125篇 |
2000年 | 123篇 |
1999年 | 93篇 |
1998年 | 56篇 |
1997年 | 45篇 |
1996年 | 60篇 |
1995年 | 56篇 |
1994年 | 56篇 |
1993年 | 37篇 |
1992年 | 64篇 |
1991年 | 57篇 |
1990年 | 32篇 |
1989年 | 42篇 |
1988年 | 42篇 |
1987年 | 53篇 |
1986年 | 45篇 |
1985年 | 33篇 |
1984年 | 34篇 |
1983年 | 34篇 |
1982年 | 28篇 |
1981年 | 27篇 |
1980年 | 23篇 |
1979年 | 25篇 |
1978年 | 30篇 |
1976年 | 22篇 |
1975年 | 22篇 |
1973年 | 22篇 |
1972年 | 20篇 |
排序方式: 共有10000条查询结果,搜索用时 467 毫秒
31.
L R Chen C J Yuan G Somasekhar P Wejksnora J E Peterson A M Myers L Graves P T Cohen E F da Cruz e Silva D J Graves 《Biochemical and biophysical research communications》1989,161(2):746-753
A cDNA encoding the entire tau subunit of rabbit skeletal muscle phosphorylase kinase was reconstructed and inserted into a plasmid containing the Escherichia coli ptac promoter and a constructed plasmid containing the ptac promoter and bacterial chloramphenicol acetyl transferase (CAT) gene, respectively. A significant phosphorylase kinase activity was found, in the first case. In the second case, a fused protein containing 73 amino acids from the CAT protein was obtained. After renaturation, the CAT-tau subunit protein shows enzymatic activity similar to the HPLC-purified and renatured tau subunit. 相似文献
32.
The major type-1 protein phosphatase catalytic subunits are the same gene products in rabbit skeletal muscle and rabbit liver 总被引:3,自引:0,他引:3
P T Cohen D L Schelling O B da Cruz e Silva H M Barker P Cohen 《Biochimica et biophysica acta》1989,1008(1):125-128
The catalytic subunit of protein phosphatase-1 (PP1) isolated from rabbit liver had the same electrophoretic mobility as, and yielded peptide maps identical to those of the 33 kDa form of rabbit skeletal muscle PP1. The predicted amino-acid sequences of PP1 obtained from three rabbit liver cDNA clones were identical to that of PP1 alpha from rabbit skeletal muscle. These findings suggest that the distinctive substrate specificities and regulatory properties of hepatic and skeletal muscle type-1 protein phosphatases are not conferred by the catalytic subunits themselves, but by regulatory subunits that are complexed to the catalytic subunits in vivo. 相似文献
33.
34.
I. B. Kerr A. M. Mendes da Silva E. Drouhet P. de Oliveira S. C. G. da Costa 《Mycopathologia》1988,101(1):3-11
Congenitally athymic nude mice (nu/nu) and their phenotypically normal littermates (nu/+) were intraperitoneally infected with yeast cells of a strain of Paracoccidioides brasiliensis. The nude mice developed a severe and generalized infection with an intense parasitism of several organs, accompanied by a low-grade of tissue reaction. The lesions were characterized by abundant yeast-like cells of the fungus, and in some animals, numerous hyphal forms could be well visualized. In control animals, infection was moderate, almost exclusively restricted to the area of inoculation, and the lesions presented few parasites surrounded by an inflammatory response. Filamentous forms of the fungus were never encountered in these animals. 相似文献
35.
β-thalassemia mutations in the Portuguese population 总被引:2,自引:0,他引:2
M. P. Coutinho Gomes M. G. Gomes da Costa L. B. Braga N. T. Cordeiro-Ferreira A. Loi M. Pirastu A. Cao 《Human genetics》1988,78(1):13-15
Summary In this study we have carried out haplotype analysis on the -globin gene cluster and characterized the -thalassemia mutation by oligonucleotide hybridization in 14 patients with thalassemia major and 5 with sickle cell/-thalassemia originating from southern Portugal. We found that three mutations, namely the °-39, ° IVS-1 nt 1 and + IVS-1 nt 110 are prevalent accounting for 53%, 32% and 10% of the -thalassemia chromosomes respectively. In general each mutation was associated with a specific chromosomal haplotype; the ° mutation, however, was linked to three different haplotypes. These results indicate that three oligo-probes complementary to the most common mutations allow prenatal diagnosis by oligonucleotide analysis in 96% of the couples at risk of having offspring with thalassemia major in southern Portugal. 相似文献
36.
Miroslav Flieger Jaroslav Votruba Vladimír Křen Sylvie Pažoutová Viktor Rylko Přemysl Sajdl Zdeněk Reháček 《Applied microbiology and biotechnology》1988,29(2-3):181-185
Summary Kinetic parameters of production of clavine alkaloids were evaluated in twoClaviceps purpurea strains. Mutagenesis brought about enhanced resistance of the biosynthetic system towards alkaloids. Addition of glucose into the fermentation medium altered the zero order kinetics of production to activation-inhibition kinetics. The glucose treatment allowed performance of both elymoclavine-inhibitionless and clavine alkaloid-decompositionless fermentations if a combination of fermentation and separation units in a closed loop was used.Nomenlacture
k
1
rate constant of agroclavine synthesis (mg Agro · mg Elymo/l·g DW·day for stage 1, mg Agro/g DW·day for stage 2)
-
k
2
parameter describing inhibition of agroclavine formation rate by elymoclavine (mg Elymo/l)
-
k
3
specific rate of agroclavine decay (l/g DW·day)
-
k
4
maximal specific rate of elymoclavine synthesis (stage 1, 1/g DW·day, stage 2, mg Elymo/g DW·day)
-
k
4
–
maximal specific rate of elymoclavine synthesis in stage 1 (inhibition-activation mechanism) (mg Elymo/g DW·day)
-
k
5
physiological constant describing the elymoclavine decay rate (l2/g DW·day·mg Elymo)
-
k
5
–
physiological constant describing the activation of elymoclavine biosynthesis by elymoclavine (mg Elymo/l)
-
k
6
physiological constant describing the repression of elymoclavine biosynthesis by elymoclavine (mg Elymo/l)
-
k
7
maximal specific growth rate (1/day)
-
k
8
specific rate of biomass decay (l/g DW·day)
-
A
agroclavine concentration (mg/l)
-
E
elymoclavine concentration (mg/l)
-
r
A
specific rate of agroclavine biosynthesis (mg Agro/g DW·day)
-
r
E
specific rate of elymoclavine biosynthesis (mg Elymo/g DW·day)
-
r
i
specific rate of alkaloid biosynthesis (mg alkaloid/g DW·day)
-
X
dry biomass concentration (g/l)
-
specific growth rate (1/day)
Abbreviations Agro
agroclavine
- Elymo
elymoclavine
- Chano
chanoclavine
- DW
dry weight of biomass 相似文献
37.
Label-fracture of cell surfaces by replica staining 总被引:2,自引:0,他引:2
We introduce replica-staining label-fracture, a method for the cytochemical mapping of membrane surfaces. This method is a corollary of the rationale of label-fracture (Pinto da Silva and Kan, 1984: J Cell Biol 99:1156). After freeze-fracture the exoplasmic halves of the membrane remain attached to the replica. We show that cytochemical labeling of cell surfaces can be performed by direct post-fracture staining of freeze-fracture replicas. This new variant of label-fracture leads to miniaturization of labeling procedures and allows standardization of labeling conditions and simultaneous processing of different specimens. 相似文献
38.
J. Šťovíčková J. Turková B. Urbánek V. Bažant J. Kavalírová 《Biotechnology Techniques》1988,2(2):121-126
Summary The effect was studied of the method of drying chymotrypsin attached to bead cellulose on its chemical and physical characteristics. These characteristics are not deteriorated when replacing the commonly used lyophilisation by fluid drying in the air stream. The preparations saved essentially the same proteolytic activity as well as stability even when increasing the drying air temperature to 70°C. 相似文献
39.
Peter Aaby Jette Bukh Ida Maria Lisse Maria Clotilde da Silva 《BMJ (Clinical research ed.)》1988,296(6631):1225-1228
The mortality from measles was studied in an urban area of Guinea-Bissau one year before and five years after the introduction of a vaccination programme. The years after the introduction of immunisation saw a decline in mortality among unvaccinated children with measles. This decline occurred despite a lower age at infection and an increasing prevalence of malnourished children. State of nutrition (weight for age) did not affect the outcome of measles infection. The incidence of isolated cases, however, increased in the period after the introduction of measles vaccination. As mortality was lower among these cases, diminished clustering explained some of the reduction in mortality. Comparison between the urban district and a rural area inhabited by the same ethnic group showed a lower age at infection, less clustering of cases, and lower case fatality ratios in the urban area.Endemic transmission of measles in urban districts leads to less clustering of cases, which may help explain the usually lower case fatality ratios in these areas. As measles vaccination increases herd immunity and diminishes clustering of cases, it may reduce mortality even among unvaccinated children who contract the disease. 相似文献
40.
Regeneration of transgenic plants of Prunus armeniaca containing the coat protein gene of Plum Pox Virus 总被引:1,自引:0,他引:1
Margit Laimer da Câmara Machado Artur da Câmara Machado Veronika Hanzer Hans Weiss Ferdinand Regner Herta Steinkellner Diethard Mattanovich Regina Plail Elisabeth Knapp Birgit Kalthoff Hermann Katinger 《Plant cell reports》1992,11(1):25-29
Summary A system was developed which allows the transfer of foreign genes into apricot cultivars. We report the transformation and regeneration of Prunus armeniaca plants with Agrobacterium tumefaciens strain LBA 4404 containing various binary plasmids, pBinGUSint, carrying the marker gene ß-glucuronidase (GUS) and pBinPPVm, carrying the coat protein gene of Plum Pox Virus (PPV). The marker gene GUS was used for optical evaluation of the efficiency of the transformation system. The coat protein gene of PPV was used to introduce coat protein mediated resistance against one of the most important pathogens of stone fruit trees in Europe and the whole Mediterranean area. This is the first report of the successful integration of a viral coat protein gene into a fruit tree species, opening a new perspective on the control of the disease.Abbreviations GUS
ß-glucuronidase
- PPV
Plum Pox Virus
- BA
6-benzylaminopurine
- NPTII
neomycin phosphotransferase II
- CP
coat protein
- CaMV
Cauliflower Mosaic Virus
- P35S 35S
promoter
- MS
Murashige and Skoog
- PCR
polymerase chain reaction
- P/C/I
phenol/chloroform/isoamylalcohol
- RNase
ribonuclease
- dNTP
deoxyribonucleosidetriphosphate
- DMSO
dimethyl sulfoxide 相似文献