排序方式: 共有30条查询结果,搜索用时 22 毫秒
11.
A. K. M. Mahbubul Alam Prakit Somta Peerasak Srinives 《Molecular breeding : new strategies in plant improvement》2014,34(3):1497-1506
Mungbean yellow mosaic India virus (MYMIV) is a major constraint on mungbean production in South and Southeast Asia. The virus belongs to the genus Begomovirus, causing yellow mosaic disease and subsequently yield loss up to 75–100 %. The present study employed F2 and BC1F1 populations derived from a cross between susceptible (BARImung 1; BM1) and resistant (BARImung 6; BM6) mungbeans to identify quantitative trait loci (QTLs) associated with resistance to MYMIV. Resistance to the virus was evaluated using F2:3 and BC1F1:2 populations under field conditions in two locations in Bangladesh in 2012. A total of 1,165 simple sequence repeat markers from different legumes were used to detect the polymorphism between BM1 and BM6. Sixty-one polymorphic markers were used to construct a linkage map comprising 11 linkage groups. Composite interval mapping consistently identified two major QTLs, qMYMIV2 on linkage group 2 and qMYMIV7 on linkage group 7, conferring the resistance in both F2 and BC1F1 populations. qMYMIV2 and qMYMIV7 accounted for 31.42–37.60 and 29.07–47.36 %, respectively, of the disease score variation, depending on populations and locations. At both loci, the resistant alleles were contributed by the parent BM6. qMYMIV2 appeared to be common to a major QTL for MYMIV resistance in mungbean reported previously, while qMYMIV7 is a new QTL for the resistance. The markers linked to the QTLs in this study are useful in marker-assisted breeding for development of mungbean varieties resistant to MYMIV. 相似文献
12.
13.
Chutintorn Yundaeng Prakit Somta Sithichoke Tangphatsornruang Sugunya Wongpornchai Peerasak Srinives 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2013,126(11):2897-2906
Key message
Sequence analysis and genetic mapping revealed that a 1,444 bp deletion causes a premature stop codon in SbBADH2 of sorghum IS19912. The non-function of SbBADH2 is responsible for fragrance in sorghum IS19912.Abstract
2-acetyl-1-pyrroline (2AP) is a potent volatile compound causing fragrance in several plants and foods. Seeds of some varieties of rice, sorghum and soybean possess fragrance. The genes responsible for fragrance in rice and soybean are orthologs that correspond to betaine aldehyde dehydrogenase 2 (BADH2). Genotypes harboring fragrance in rice and soybean contain a premature stop codon in BADH2 which impairs the synthesis of full length functional BADH2 protein leading to the accumulation of 2AP. In this study, we reported an association between the BADH2 gene and fragrance in sorghum. An F2 population of 187 plants developed from a cross between KU630 (non-fragrant) and IS19912 (fragrant) was used. Leaves of F2 and F3 progenies were evaluated for fragrance by organoleptic test, while seeds of F2 plants were analyzed for 2AP. The tests consistently showed that the fragrance is controlled by a single recessive gene. Gene expression analysis of SbBADH1 and SbBADH2 in leaves of KU630 and IS19912 at various stages revealed that SbBADH1 and SbBADH2 were expressed in both accessions. Sequence comparison between KU630 and IS19912 revealed a continuous 1,444 bp deletion encompassing exon 12 to 15 of SbBADH2 in IS19912 which introduces a frameshift mutation and thus causes a premature stop codon. An indel marker was developed to detect polymorphism in SbBADH2. Bulk segregant and QTL analyses confirmed the association between SbBADH2 and fragrance. 相似文献14.
Juwattanasomran R Somta P Chankaew S Shimizu T Wongpornchai S Kaga A Srinives P 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,122(3):533-541
Fragrance in soybean is due to the presence of 2-acetyl-1-pyrroline (2AP). BADH2 gene coding for betaine aldehyde dehydrogenase has been identified as the candidate gene responsible for fragrance in rice (Oryza sativa L.). In this study, using the RIL population derived from fragrant soybean cultivar "Kaori" and non-fragrant soybean cultivar "Chiang Mai 60" (CM60), STS markers designed from BADH2 homolog were found associating with 2AP production. Genetic mapping demonstrated that QTL position of fragrance and 2AP production coincides with the position of GmBADH2 (Glycine max betaine aldehyde dehydrogenase 2). Sequence comparison of GmBADH2 between Kaori and non-fragrant soybeans revealed non-synonymous single-nucleotide polymorphism (SNP) in exon 10. Nucleotide substitution of G to A in the exon results in an amino acid change of glycine (GGC; G) to aspartic acid (GAC; D) in Kaori. The amino acid substitution changes the conserved EGCRLGPIVS motif of GmBADH2, which is essential for functional activity of GmBADH2 protein, to EGCRLDPIVS motif, suggesting that the SNP in GmBADH2 is responsible for the fragrance in Kaori. Five single nucleotide-amplified polymorphism (SNAP) markers which are PCR-based allele specific SNP markers were developed for fragrance based on the SNP in GmBADH2. Two markers specific to A allele produced a band in only Kaori, while three markers specific to G alleles produced a band in only CM60. The simple PCR-based allele specific SNAP markers developed in the present study are useful in marker-assisted breeding of fragrant soybean. 相似文献
15.
Sompong Chankaew Takehisa Isemura Sachiko Isobe Akito Kaga Norihiko Tomooka Prakit Somta Hideki Hirakawa Kenta Shirasawa Duncan A. Vaughan Peerasak Srinives 《PloS one》2014,9(8)
Vigna reflexo-pilosa, which includes a neglected crop, is the only one tetraploid species in genus Vigna. The ancestral species that make up this allotetraploid species have not conclusively been identified, although previous studies suggested that a donor genome of V. reflexo-pilosa is V. trinervia. In this study, 1,429 azuki bean EST-SSR markers were developed of which 38 EST-SSR primer pairs that amplified one product in diploid species and two discrete products in tetraploid species were selected to analyze 268 accessions from eight taxa of seven Asian Vigna species including V. reflexo-pilosa var. glabra, V. reflexo-pilosa var. reflexo-pilosa, V. exilis, V. hirtella, V. minima, V. radiata var. sublobata, V. tenuicaulis and V. trinervia to identify genome donor of V. reflexo-pilosa. Since both diploid and tetraploid species were analyzed and each SSR primer pair detected two loci in the tetraploid species, we separated genomes of the tetraploid species into two different diploid types, viz. A and B. In total, 445 alleles were detected by 38 EST-SSR markers. The highest gene diversity was observed in V. hirtella. By assigning the discrete PCR products of V. reflexo-pilosa into two distinguished genomes, we were able to identify the two genome donor parents of créole bean. Phylogenetic and principal coordinate analyses suggested that V. hirtella is a species complex and may be composed of at least three distinct taxa. Both analyses also clearly demonstrated that V. trinervia and one taxon of V. hirtella are the genome donors of V. reflexo-pilosa. Gene diversity indicates that the evolution rate of EST-SSRs on genome B of créole bean might be faster than that on genome A. Species relationship among the Vigna species in relation to genetic data, morphology and geographical distribution are presented. 相似文献
16.
Water footprint of livestock: comparison of six geographically defined beef production systems 总被引:2,自引:0,他引:2
Bradley G. Ridoutt Peerasak Sanguansri Michael Freer Gregory S. Harper 《The International Journal of Life Cycle Assessment》2012,17(2):165-175
Purpose
Water use in the livestock sector has featured in the debate about sustainable food systems. Most evidence has come from virtual water calculations which lack impact assessment and adequate consideration of the heterogeneity in livestock production. This study sought new evidence, using a recently developed life cycle impact assessment method for water use to assess six geographically defined beef cattle production systems in New South Wales, Australia, a major production region. 相似文献17.
Somta P Musch W Kongsamai B Chanprame S Nakasathien S Toojinda T Sorajjapinun W Seehalak W Tragoonrung S Srinives P 《Molecular ecology resources》2008,8(5):1155-1157
In this study, we reported the isolation and analysis of new polymorphic microsatellites in mungbean (Vigna radiata (L.) Wilczek). Twelve out of 210 primer pairs screened in 30 mungbean accessions gave polymorphism. The polymorphic markers detected two to three alleles per locus with an average of 2.08. Observed heterozygosity varied from 0 to 0.133, while expected heterozygosity ranged from 0.095 to 0.498. Tests for Hardy-Weinberg equilibrium (HWE) and pairwise linkage disequilibrium of the polymorphic loci revealed that all loci except MB-SSR14 significantly departed from HWE and four pairwise combinations, viz. MB-SSR14 vs. MB-SSR42, MB-SSR42 vs. MB-SSR87, MB-SSR114 vs. MB-SSR121, and MB-SSR175 vs. MB-SSR231 significantly deviated from linkage disequilibrium. The markers are being used to study genetic diversity and genome mapping of mungbean. 相似文献
18.
Jungmin Ha Sangrea Shim Taeyoung Lee Yang J. Kang Won J. Hwang Haneul Jeong Kularb Laosatit Jayern Lee Sue K. Kim Dani Satyawan Puji Lestari Min Y. Yoon Moon Y. Kim Annapurna Chitikineni Patcharin Tanya Prakit Somta Peerasak Srinives Rajeev K. Varshney Suk‐Ha Lee 《Plant biotechnology journal》2019,17(2):517-530
19.
20.
QTL identification of flowering time at three different latitudes reveals homeologous genomic regions that control flowering in soybean 总被引:1,自引:0,他引:1
Liu W Kim MY Kang YJ Van K Lee YH Srinives P Yuan DL Lee SH 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》2011,123(4):545-553
Since the genetic control of flowering time is very important in photoperiod-sensitive soybean (Glycine max (L.) Merr.), genes affecting flowering under different environment conditions have been identified and described. The objectives
were to identify quantitative trait loci (QTLs) for flowering time in different latitudinal and climatic regions, and to understand
how chromosomal rearrangement and genome organization contribute to flowering time in soybean. Recombinant inbred lines from
a cross between late-flowering ‘Jinpumkong 2’ and early-flowering ‘SS2-2’ were used to evaluate the phenotypic data for days
to flowering (DF) collected from Kamphaeng Saen, Thailand (14°01′N), Suwon, Korea (37°15′N), and Longjing, China (42°46′N).
A weakly positive phenotypic correlation (r = 0.36) was found between DF in Korea and Thailand; however, a strong correlation (r = 0.74) was shown between Korea and China. After 178 simple sequence repeat (SSR) markers were placed on a genetic map spanning
2,551.7 cM, four independent DF QTLs were identified on different chromosomes (Chrs). Among them, three QTLs on Chrs 9, 13
and 16 were either Thailand- or Korea-specific. The DF QTL on Chr 6 was identified in both Korea and China, suggesting it
is less environment-sensitive. Comparative analysis of four DF QTL regions revealed a syntenic relationship between two QTLs
on Chrs 6 and 13. All five duplicated gene pairs clustered in the homeologous genomic regions were found to be involved in
the flowering. Identification and comparative analysis of multiple DF QTLs from different environments will facilitate the
significant improvement in soybean breeding programs with respect to control of flowering time. 相似文献