全文获取类型
收费全文 | 630篇 |
免费 | 103篇 |
出版年
2022年 | 10篇 |
2021年 | 24篇 |
2020年 | 5篇 |
2019年 | 3篇 |
2018年 | 12篇 |
2017年 | 10篇 |
2016年 | 19篇 |
2015年 | 30篇 |
2014年 | 37篇 |
2013年 | 34篇 |
2012年 | 56篇 |
2011年 | 43篇 |
2010年 | 36篇 |
2009年 | 22篇 |
2008年 | 40篇 |
2007年 | 40篇 |
2006年 | 42篇 |
2005年 | 49篇 |
2004年 | 30篇 |
2003年 | 46篇 |
2002年 | 32篇 |
2001年 | 14篇 |
2000年 | 10篇 |
1999年 | 7篇 |
1998年 | 5篇 |
1997年 | 5篇 |
1996年 | 8篇 |
1995年 | 10篇 |
1994年 | 7篇 |
1993年 | 8篇 |
1992年 | 6篇 |
1991年 | 3篇 |
1990年 | 11篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 3篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1981年 | 1篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1966年 | 2篇 |
1960年 | 1篇 |
排序方式: 共有733条查询结果,搜索用时 46 毫秒
61.
Yilmaz P Kottmann R Field D Knight R Cole JR Amaral-Zettler L Gilbert JA Karsch-Mizrachi I Johnston A Cochrane G Vaughan R Hunter C Park J Morrison N Rocca-Serra P Sterk P Arumugam M Bailey M Baumgartner L Birren BW Blaser MJ Bonazzi V Booth T Bork P Bushman FD Buttigieg PL Chain PS Charlson E Costello EK Huot-Creasy H Dawyndt P DeSantis T Fierer N Fuhrman JA Gallery RE Gevers D Gibbs RA San Gil I Gonzalez A Gordon JI Guralnick R Hankeln W Highlander S Hugenholtz P Jansson J Kau AL Kelley ST 《Nature biotechnology》2011,29(5):415-420
Here we present a standard developed by the Genomic Standards Consortium (GSC) for reporting marker gene sequences--the minimum information about a marker gene sequence (MIMARKS). We also introduce a system for describing the environment from which a biological sample originates. The 'environmental packages' apply to any genome sequence of known origin and can be used in combination with MIMARKS and other GSC checklists. Finally, to establish a unified standard for describing sequence data and to provide a single point of entry for the scientific community to access and learn about GSC checklists, we present the minimum information about any (x) sequence (MIxS). Adoption of MIxS will enhance our ability to analyze natural genetic diversity documented by massive DNA sequencing efforts from myriad ecosystems in our ever-changing biosphere. 相似文献
62.
Audenaert P Van Parys T Brondel F Pickavet M Demeester P Van de Peer Y Michoel T 《Bioinformatics (Oxford, England)》2011,27(11):1587-1588
SUMMARY: Network motifs in integrated molecular networks represent functional relationships between distinct data types. They aggregate to form dense topological structures corresponding to functional modules which cannot be detected by traditional graph clustering algorithms. We developed CyClus3D, a Cytoscape plugin for clustering composite three-node network motifs using a 3D spectral clustering algorithm. AVAILABILITY: Via the Cytoscape plugin manager or http://bioinformatics.psb.ugent.be/software/details/CyClus3D. 相似文献
63.
Comparative genomics of the mating-type loci of the mushroom Flammulina velutipes reveals widespread synteny and recent inversions 总被引:1,自引:0,他引:1
van Peer AF Park SY Shin PG Jang KY Yoo YB Park YJ Lee BM Sung GH James TY Kong WS 《PloS one》2011,6(7):e22249
Background
Mating-type loci of mushroom fungi contain master regulatory genes that control recognition between compatible nuclei, maintenance of compatible nuclei as heterokaryons, and fruiting body development. Regions near mating-type loci in fungi often show adapted recombination, facilitating the generation of novel mating types and reducing the production of self-compatible mating types. Compared to other fungi, mushroom fungi have complex mating-type systems, showing both loci with redundant function (subloci) and subloci with many alleles. The genomic organization of mating-type loci has been solved in very few mushroom species, which complicates proper interpretation of mating-type evolution and use of those genes in breeding programs.Methodology/Principal Findings
We report a complete genetic structure of the mating-type loci from the tetrapolar, edible mushroom Flammulina velutipes mating type A3B3. Two matB3 subloci, matB3a that contains a unique pheromone and matB3b, were mapped 177 Kb apart on scaffold 1. The matA locus of F. velutipes contains three homeodomain genes distributed over 73 Kb distant matA3a and matA3b subloci. The conserved matA region in Agaricales approaches 350 Kb and contains conserved recombination hotspots showing major rearrangements in F. velutipes and Schizophyllum commune. Important evolutionary differences were indicated; separation of the matA subloci in F. velutipes was diverged from the Coprinopsis cinerea arrangement via two large inversions whereas separation in S. commune emerged through transposition of gene clusters.Conclusions/Significance
In our study we determined that the Agaricales have very large scale synteny at matA (∼350 Kb) and that this synteny is maintained even when parts of this region are separated through chromosomal rearrangements. Four conserved recombination hotspots allow reshuffling of large fragments of this region. Next to this, it was revealed that large distance subloci can exist in matB as well. Finally, the genes that were linked to specific mating types will serve as molecular markers in breeding. 相似文献64.
The fate of host defensive behaviour in the absence of selection from brood parasitism is critical to long-term host-parasite coevolution. We investigated whether New World Bohemian waxwings Bombycilla garrulus that are allopatric from brown-headed cowbird Molothrus ater and common cuckoo Cuculus canorus parasitism have retained egg rejection behaviour. We found that egg rejection was expressed by 100 per cent of Bohemian waxwings. Our phylogeny revealed that Bohemian and Japanese waxwings Bombycilla japonica were sister taxa, and this clade was sister to the cedar waxwing Bombycilla cedrorum. In addition, there was support for a split between Old and New World Bohemian waxwings. Our molecular clock estimates suggest that egg rejection may have been retained for 2.8-3.0 Myr since New World Bohemian waxwings inherited it from their common ancestor with the rejecter cedar waxwings. These results support the 'single trajectory' model of host-brood parasite coevolution that once hosts evolve defences, they are retained, forcing parasites to become more specialized over time. 相似文献
65.
DNA encodes at least two independent levels of functional information. The first level is for encoding proteins and sequence targets for DNA-binding factors, while the second one is contained in the physical and structural properties of the DNA molecule itself. Although the physical and structural properties are ultimately determined by the nucleotide sequence itself, the cell exploits these properties in a way in which the sequence itself plays no role other than to support or facilitate certain spatial structures. In this work, we focus on these structural properties, comparing them between different organisms and assessing their ability to describe the core promoter. We prove the existence of distinct types of core promoters, based on a clustering of their structural profiles. These results indicate that the structural profiles are much conserved within plants (Arabidopsis and rice) and animals (human and mouse), but differ considerably between plants and animals. Furthermore, we demonstrate that these structural profiles can be an alternative way of describing the core promoter, in addition to more classical motif or IUPAC-based approaches. Using the structural profiles as discriminatory elements to separate promoter regions from non-promoter regions, reliable models can be built to identify core-promoter regions using a strictly computational approach. 相似文献
66.
Genome duplication and the origin of angiosperms 总被引:9,自引:0,他引:9
Despite intensive research, little is known about the origin of the angiosperms and their rise to ecological dominance during the Early Cretaceous. Based on whole-genome analyses of Arabidopsis thaliana, there is compelling evidence that angiosperms underwent two whole-genome duplication events early during their evolutionary history. Recent studies have shown that these events were crucial for the creation of many important developmental and regulatory genes found in extant angiosperm genomes. Here, we argue that these ancient polyploidy events might have also had an important role in the origin and diversification of the angiosperms. 相似文献
67.
Repressor- and activator-type ethylene response factors functioning in jasmonate signaling and disease resistance identified via a genome-wide screen of Arabidopsis transcription factor gene expression 总被引:13,自引:0,他引:13
下载免费PDF全文
![点击此处可从《Plant physiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
McGrath KC Dombrecht B Manners JM Schenk PM Edgar CI Maclean DJ Scheible WR Udvardi MK Kazan K 《Plant physiology》2005,139(2):949-959
68.
Medusa: a simple tool for interaction graph analysis 总被引:5,自引:0,他引:5
SUMMARY: Medusa is a Java application for visualizing and manipulating graphs of interaction, such as data from the STRING database. It features an intuitive user interface developed with the help of biologists. Medusa is optimized for accessing protein interaction data from STRING, but can be used for any type of graph from any scientific field. 相似文献
69.
70.