全文获取类型
收费全文 | 7067篇 |
免费 | 511篇 |
国内免费 | 2篇 |
专业分类
7580篇 |
出版年
2023年 | 69篇 |
2022年 | 131篇 |
2021年 | 204篇 |
2020年 | 148篇 |
2019年 | 213篇 |
2018年 | 228篇 |
2017年 | 217篇 |
2016年 | 277篇 |
2015年 | 401篇 |
2014年 | 385篇 |
2013年 | 522篇 |
2012年 | 555篇 |
2011年 | 611篇 |
2010年 | 389篇 |
2009年 | 311篇 |
2008年 | 408篇 |
2007年 | 393篇 |
2006年 | 344篇 |
2005年 | 307篇 |
2004年 | 278篇 |
2003年 | 259篇 |
2002年 | 226篇 |
2001年 | 70篇 |
2000年 | 48篇 |
1999年 | 53篇 |
1998年 | 46篇 |
1997年 | 48篇 |
1996年 | 39篇 |
1995年 | 34篇 |
1994年 | 22篇 |
1993年 | 26篇 |
1992年 | 34篇 |
1991年 | 22篇 |
1990年 | 23篇 |
1989年 | 15篇 |
1988年 | 23篇 |
1987年 | 15篇 |
1986年 | 9篇 |
1985年 | 10篇 |
1984年 | 15篇 |
1983年 | 16篇 |
1982年 | 12篇 |
1981年 | 18篇 |
1980年 | 14篇 |
1979年 | 10篇 |
1978年 | 6篇 |
1977年 | 14篇 |
1976年 | 16篇 |
1975年 | 12篇 |
1974年 | 5篇 |
排序方式: 共有7580条查询结果,搜索用时 15 毫秒
91.
H2AX phosphorylation at serine 139 (γH2AX) is a sensitive indicator of both DNA damage and DNA replication stress. Here we show that γH2AX formation is greatly enhanced in response to replication inhibitors but not ionizing radiation in HCT116 or SW480 cells depleted of Chk1. Although H2AX phosphorylation precedes the induction of apoptosis in such cells, our results suggest that cells containing γH2AX are not committed to death. γH2AX foci in these cells largely colocalize with RPA foci and their formation is dependent upon the essential replication helicase cofactor Cdc45, suggesting that H2AX phosphorylation occurs at sites of stalled forks. However Chk1-depleted cells released from replication inhibitors retain γH2AX foci and do not appear to resume replicative DNA synthesis. BrdU incorporation only occurs in a minority of Chk1-depleted cells containing γH2AX foci after release from thymidine arrest and, in cells incorporating BrdU, DNA synthesis does not occur at sites of γH2AX foci. Furthermore activated ATM and Chk2 persist in these cells. We propose that the γH2AX foci in Chk1-depleted cells may represent sites of persistent replication fork damage or abandonment that are unable to resume DNA synthesis but do not play a direct role in the Chk1 suppressed death pathway. 相似文献
92.
93.
Alvarez I Bengochea M Toledo R Carretto E Hidalgo PC 《Human biology; an international record of research》2006,78(4):513-525
HLA class I antigens were determined for 959 unrelated Uruguayans. The predominant HLA alleles were A2, Cw4, and B35, and the most frequently observed two-loci haplotypes were A2-B44 and B35-Cw4. The most frequent three-loci HLA haplotype was A2-Cw5-B44. We compared the Uruguayan sample with similar data from other populations. 相似文献
94.
Bibiana S. O. Fam Guillermo Reales Pedro Vargas‐Pinilla Pamela Par Lucas H. Viscardi Vinicius A. Sortica Aline B. Felkl lvaro de O. Franco Aldo B. Lucion Claudio M. Costa‐Neto Alcides Pissinatti Francisco M. Salzano Vanessa R. Paixo‐Crtes Maria Ctira Bortolini 《American journal of primatology》2019,81(8)
Platyrrhini (New World monkeys, NWm) are a group of primates characterized by behavioral and reproductive traits that are otherwise uncommon among primates, including social monogamy, direct paternal care, and twin births. As a consequence, the study of Platyrrhine primates is an invaluable tool for the discovery of the genetic repertoire underlying these taxon‐specific traits. Recently, high conservation of vasopressin (AVP) sequence, in contrast with high variability of oxytocin (OXT), has been described in NWm. AVP and OXT functions are possible due to interaction with their receptors: AVPR1a, AVPR1b, AVPR2, and OXTR; and the variability in this system is associated with the traits mentioned above. Understanding the variability in the receptors is thus fundamental to understand the function and evolution of the system as a whole. Here we describe the variability of AVPR1b coding region in 20 NWm species, which is well‐known to influence behavioral traits such as aggression, anxiety, and stress control in placental mammals. Our results indicate that 4% of AVPR1b sites may be under positive selection and a significant number of sites under relaxed selective constraint. Considering the known role of AVPR1b, we suggest that some of the changes described here for the Platyrrhini may be a part of the genetic repertoire connected with the complex network of neuroendocrine mechanisms of AVP–OXT system in the modulation of the HPA axis. Thus, these changes may have promoted the emergence of social behaviors such as direct paternal care in socially monogamous species that are also characterized by small body size and twin births. 相似文献
95.
96.
In situ hybridization was used to determine the physical location of the Hsp70 genes in salivary polytene chromosomes of Anopheles darlingi from Manaus and Macapá, Brazil, and to assess the usefulness of the Hsp70 locus as a genetic marker in A. darlingi populations. In both populations, the double markings corresponding to the Hsp70-12A and Hsp70-14A genes were located on the right arm of chromosome 2. The Hsp70 locus was considered to be an excellent marker for studying chromosomal evolution and relationships among A. darlingi populations. 相似文献
97.
Vnia Salgueiro Carmo Silva Sofia Eufrzio Pedro A. Salgueiro Pedro G. Vaz 《Restoration Ecology》2020,28(3):668-678
As practitioners promote passive restoration as a complementary approach to technical reclamation, it is imperative to know its drivers. Although the consequences of endozoochory can be crucial to passive restoration success, few experimental studies assess the use of heavily disturbed sites by seed dispersers such as carnivores and how the seeds they bring in emerge and survive. Using an indoor sowing experiment conducted in a quarry located within a natural park in Portugal, we examined for the first time how carnivore endozoochorous seeds collected in the quarry potentially influence its passive restoration, through effects on plant emergence and survival. Also, we tested whether sowing date and water soaking, relevant factors when sowings are to be carried out, would affect seedling emergence and mortality rates when compared with the effect of endozoochory. Our target species were included in the revegetation plan of the quarry, of which endozoochorous seeds of Carob tree (Ceratonia siliqua) were collected in sufficient number for analysis. Irrespective of the carnivore species, endozoochorous carob seeds performed similarly to untreated seeds regarding emergence rates. Endozoochorous carob seedlings showed greater mortality rates but the net result for the plant can still be the colonization of recently vacant habitats by a large proportion of viable seeds. Concerning sowing date, the later carob seeds were sown over the fruit‐ripening season the faster seedlings emerged. Water soaking increased emergence rate by 6.5 times. Broadly, sowings with previous soaking and carnivore‐mediated seed dispersal of this dry‐fruited tree can jointly enhance quarry restoration. 相似文献
98.
Theoretical and empirical evidence suggests that avian females are able to manipulate the offspring sex ratio at birth. Although
mating with an attractive male may induce females to skew the sex ratio toward males, the balance between the benefits of
producing attractive sons and the costs of competing with other females for mates could vary with female age, a possibility
that had not been previously explored. In this paper we increment experimentally the attractiveness of males of the polygynous
spotless starling (Sturnus unicolor) by adding green plants to their nests, a trait involved in courtship, and look for female age-differential effects on offspring
primary sex ratio. Young and middle aged females produced more sons in experimental than in control nests, as expected, but
old females showed the opposite tendency. To explain this novel result, we speculate that older females are limited to produce
the most costly sex because the physiological drawbacks imposed by ageing reduce their ability to compete with younger ones
for the non-shareable resources offered by males. We discuss that this evolutionary scenario may be widespread in avian polygynous
systems. 相似文献
99.
Wassink L Vieira PL Smits HH Kingsbury GA Coyle AJ Kapsenberg ML Wierenga EA 《Journal of immunology (Baltimore, Md. : 1950)》2004,173(3):1779-1786
Previous mouse studies have shown that IL-4 increases the expression of ICOS on activated Th cells, resulting in enhanced ICOS expression on Th2 cells. In this study, we show that ICOS expression on human Th cells is not increased by IL-4, but by IL-12 and by IL-23 instead. Consequently, ICOS expression during IL-12-driven Th1 cell polarization was transiently increased compared with the levels on Th0 cells and IL-4-driven Th2 cells. Addition of IL-12 and/or IL-23 during restimulation increased ICOS expression to the same extent on pre-established Th1, Th2, and Th0 cells, indicating that ICOS levels are not stably imposed by prior polarization. In contrast to the findings in the mouse, IL-4 significantly suppressed the ICOS-enhancing effects of IL-12 and IL-23. The functional consequence of variable ICOS levels was shown in coculture experiments with cells expressing the ICOS-ligand B7-related protein 1 (either transfected Chinese hamster ovary cells or autologous dendritic cells). Ligation of ICOS on 2-day-preactivated effector cells increased their cytokine production to an extent proportional to their ICOS expression levels. As the ICOS-enhancing potentials of IL-12 and IL-23 were maintained for several days after stimulation, both on Th1 and Th2 cells, we propose the concept that local regulation of ICOS expression on activated Th cells by IL-12 and/or IL-23 may provide a powerful means to amplify effector T cell responses in peripheral tissues, independently of the polarized state of the Th cells. 相似文献
100.
Symptoms induced by transgenic expression of p23 from Citrus tristeza virus in phloem‐associated cells of Mexican lime mimic virus infection without the aberrations accompanying constitutive expression 下载免费PDF全文
Nuria Soler Carmen Fagoaga Carmelo López Pedro Moreno Luis Navarro Ricardo Flores Leandro Peña 《Molecular Plant Pathology》2015,16(4):388-399
Citrus tristeza virus (CTV) is phloem restricted in natural citrus hosts. The 23‐kDa protein (p23) encoded by the virus is an RNA silencing suppressor and a pathogenicity determinant. The expression of p23, or its N‐terminal 157‐amino‐acid fragment comprising the zinc finger and flanking basic motifs, driven by the constitutive 35S promoter of cauliflower mosaic virus, induces CTV‐like symptoms and other aberrations in transgenic citrus. To better define the role of p23 in CTV pathogenesis, we compared the phenotypes of Mexican lime transformed with p23‐derived transgenes from the severe T36 and mild T317 CTV isolates under the control of the phloem‐specific promoter from Commelina yellow mottle virus (CoYMV) or the 35S promoter. Expression of the constructs restricted to the phloem induced a phenotype resembling CTV‐specific symptoms (vein clearing and necrosis, and stem pitting), but not the non‐specific aberrations (such as mature leaf epinasty and yellow pinpoints, growth cessation and apical necrosis) observed when p23 was ectopically expressed. Furthermore, vein necrosis and stem pitting in Mexican lime appeared to be specifically associated with p23 from T36. Phloem‐specific accumulation of the p23Δ158–209(T36) fragment was sufficient to induce the same anomalies, indicating that the region comprising the N‐terminal 157 amino acids of p23 is responsible (at least in part) for the vein clearing, stem pitting and, possibly, vein corking in this host. 相似文献