Evaluation of zona pellucida birefringence intensity during in vitro maturation of oocytes from stimulated cycles

Background  

This study evaluated whether there is a relationship between the zona pellucida birefringence (ZP-BF) intensity and the nuclear (NM) and cytoplasmic (CM) in vitro maturation of human oocytes from stimulated cycles.     

氦氧饱和高气压暴露对铜绿假单胞菌毒力表型的诱导调节

研究氦氧饱和高气压暴露对铜绿假单胞菌与急性侵袭感染相关的主要毒力因子表型的影响。分别检测暴露前后铜绿假单胞菌的生长速度、运动能力,对乙酰胺的分解能力和培养细胞外液中绿脓菌素、弹性蛋白酶的分泌水平和活性。在动物水平验证毒力的变化。结果显示,经4.5 MPa氦氧饱和加压暴露后,与对照组相比铜绿假单胞菌的生长增殖、运动能力和对乙酰胺的分解能力增强,绿脓菌素分泌增加,弹性蛋白酶活性增强;暴露后细菌对实验小鼠的毒性增加。因此氦氧饱和高气压环境对铜绿假单胞菌的毒力表型有正调控作用。     

IL-18通过MyD88依赖信号途径调控CSF-1表达

IL-18是IL-1家族的一个成员,最初发现被命名为IFN-γ诱导因子.现在研究表明,IL-18是炎症反应的重要标志,参与许多慢性炎症和自身免疫性疾病炎性反应.证明了IL-18在JurkatT淋巴细胞的炎症反应中能够调节CSF-1的表达,其信号机制是IL-18通过MyD88-NF-κB-CSF-1信号途径上调CSF-1的基因转录.     

污染环境下单种群模型生存阈值

本论文研究了污染环境下毒素对单种群生存的影响。在环境容纳量较小的假设下建立了生物种群模型,在该模型中不但考虑了环境毒素浓度对生物个体生存的影响,还考虑了生物个体从食物链中吸收的毒素对其影响。通过研究得到种群一致持续生存和若平均持续生存的充分条件,同时得到种群持续生存依赖于模型参数和生物个体体内毒素净化率的某些充分条件．     

双源CT 冠状动脉血管成像诊断心肌桥的价值探讨

目的：研究双源CT 冠状动脉血管成像诊断心肌桥的临床价值。方法：选择260 例具有典型心前区不适的患者进行双源CT 冠脉血管成像检查,观察其发生部位,测量其长度和深度并进行分析。结果：260 例受检患者中,62例共70 段存在心肌桥,检出率 达20.76%,高于文献报道的检出率18.2%。所有心肌桥均发生于左前降支,其中近段17 段(24.4%),中段43 段(61.4%),远段10 段 (14.2%)。心肌桥平均长度为15.8± 6.4mm,深度为1.4± 0.85mm。结论：双源CT 冠状动脉血管成像因其便捷无创,不受心率严格 限制且价格低廉可作为心肌桥筛查的理想检查手段。     

Fibroblast growth factor family aberrations in cancers: clinical and molecular characteristics

Fibroblast growth factor ligands and receptors (FGF and FGFR) play critical roles in tumorigenesis, and several drugs have been developed to target them. We report the biologic correlates of FGF/FGFR abnormalities in diverse malignancies. The medical records of patients with cancers that underwent targeted next generation sequencing (182 or 236 cancer-related genes) were reviewed. The following FGF/FGFR genes were tested: FGF3, 4, 6, 7, 10, 12, 14, 19, 23 and FGFR1, 2, 3, and 4. Of 391 patients, 56 (14.3%) had aberrant FGF (N = 38, all amplifications) and/or FGFR (N = 22 including 5 mutations and one FGFR3-TACC3 fusion). FGF/FGFR aberrations were most frequent in breast cancers (26/81, 32.1%, p = 0.0003). In multivariate analysis, FGF/FGFR abnormalities were independently associated with CCND1/2, RICTOR, ZNF703, RPTOR, AKT2, and CDK8 alterations (all P < 0.02), as well as with an increased median number of alterations (P < 0.0001). FGF3, FGF4, FGF19 and CCND1 were co-amplified in 22 of 391 patients (5.6%, P < 0.0001), most likely because they co-localize on the same chromosomal region (11q13). There was no significant difference in time to metastasis or overall survival when comparing patients harboring FGF/FGFR alterations versus those not. Overall, FGF/FGFR was one of the most frequently aberrant pathways in our population comprising patients with diverse malignancies. These aberrations frequently co-exist with anomalies in a variety of other genes, suggesting that tailored combination therapy may be necessary in these patients.     

18F-FDG PET和治疗量131I全身扫描对分化型甲状腺癌根治术后转移灶检测的临床价值评估

目的：探讨18F-FDG PET显像和131I-全身扫描（131I-WBS）SPECT显像对分化型甲状腺癌（DTC）术后转移灶的临床诊断价值。方法：对57例外科术后拟行131I治疗的DTC患者行18F-FDG PET全身显像和131I-WBS扫描,观察和记录在糖代谢和碘代谢中DTC转移灶的定位及数量变化,并同时测定甲状腺球蛋白（Tg）,促甲状腺激素释放激素（TSH）等实验室检查项目。结果：57例DTC患者18F-FDG PET显像发现真阳性20例、假阳性3例、真阴性31例、假阴性3例,其灵敏度为87.0%,特异性为91.2%。而131I-WBS扫描发现真阳性13例、假阳性2例、真阴性34例、假阴性8例,其灵敏度为61.9%,特异性为94.4%。PET显像和131I-WBS扫描共检出阳性病灶73个,其中淋巴结32个,肺5个,纵隔6个,骨26个,其他部位4个。PET显像发现43个阳性病灶（58.9%）,而131I-WBS检出30个（41.1%）。当Tg水平〉10μg/L时,随着Tg在血清含量的增高,两种显像方法的对DTC转移灶的阳性检出率亦随之升高。结论：两种检查对DTC术后转移灶的监测和131I的治疗具有良好的互补性,18F-FDG PET显像在Tg阳性和131I-WBS阴性的患者的转移灶检出上更具有优势,有重要的临床指导意义。     

猪CFL2b 基因cDNA克隆初步分析

利用基因表达谱芯片分析法筛选出与大白猪高肌肉产量-肌纤维形成有关的CFL2b基因.参考人和小鼠CFL2b基因序列,采用SMART-RACE技术结合EST序列拼接技术,从猪骨骼肌肌肉中首次克隆了猪CFL2b的全长cDNA序列（GenBank登录号EU561660,EU561661）,Northern杂交检测CFL2b基因 mRNA.结果表明,猪CFL2b基因含有2个转录本,长转录本3　012 bp,短转录本1　466 bp .CFL2b基因在多种真核生物中都有表达,且编码区序列非常保守,开放式读码框501 bp编码了166个氨基酸的蛋白质.氨基酸序列分析表明,猪CFL2基因与人和小鼠氨基酸同源性分别为100%和99.1%.核苷酸序列相似性分别为88.1%和74.9%.     

Further evidence of the African antiquity of hyaenodontid (‘Creodonta’, Mammalia) evolution

We report here the new ‘creodont’ Lahimia selloumi gen. et sp. nov. from the late Palaeocene of the Ouled Abdoun Basin (Morocco) as the oldest known Hyaenodontidae with Tinerhodon from the Ouarzazate Basin (Morocco). By contrast to Tinerhodon, Lahimia is unexpectedly derived. Most of its specializations, such as the shortening of the anterior dentition (e.g. loss of P₁) and the talonid reduction and simplification, are strikingly shared with Boualitomus from the Ypresian of the Ouled Abdoun Basin, and are distinctive from other hyaenodontids, including ‘proviverrines’. They are interpreted as synapomorphies evidencing a precociously specialized early African hyaenodontid lineage. Although Lahimia and Boualitomus remain known only by the lower dentition, their relationships with Koholia are suggested by comparison of their molar occlusal pattern. Lahimia and Boualitomus are referred to the Koholiinae, which is representative of an old African endemic lineage, as initially recognized. This remarkable lineage is characterized by synapomorphies of Lahimia and Boualitomus, and also by a shared original prevallum/postvallid shearing. The discovery of Lahimia provides direct evidence for the antiquity of the African evolution of the Hyaenodontidae. This is in agreement with an African origin of the Hyaenodontidae, and with the probable diphyletism of the ‘Creodonta’. Lahimia and the Koholiinae, as well as the diversity of the first Laurasian hyaenodontid lineages, emphasize our poor knowledge of the striking early African hyaenodontid radiation.     

Genetic diversity and differentiation in natural and reintroduced populations of Bencomia exstipulata and comparisons with B. caudata (Rosaceae) in the Canary Islands: an analysis using microsatellites

Variation at five polymorphic microsatellite loci was used to investigate genetic diversity and differentiation of two tetraploid Canarian endemics, Bencomia exstipulata and B. caudata. Data were analysed and are discussed in terms of tetrasomic (autotetraploid) and disomic (allotetraploid) inheritance. In both cases, genetic diversity values were similar to those described in other tetraploid plant species. High genetic differentiation between the only two described natural populations of B. exstipulata was detected (F_ST = 0.411). Bayesian cluster analysis revealed a geographical structure with distinct genetic groups from each island. High genetic differentiation and low genetic diversity of the B. exstipulata population from Tenerife suggest a recent population bottleneck, perhaps caused by the most recent major volcanic eruption, for this natural locality. This may be heightened by possible inbreeding depression and the monoecy of these species. Polymorphic microsatellite loci were also tested across all species in the Bencomia alliance. These reliably amplified the target sequence, suggesting a high degree of conservation of the sequences flanking the microsatellites. © 2009 The Linnean Society of London, Botanical Journal of the Linnean Society, 2009, 160 , 429–441.