全文获取类型
收费全文 | 1162篇 |
免费 | 50篇 |
专业分类
1212篇 |
出版年
2023年 | 5篇 |
2022年 | 5篇 |
2021年 | 13篇 |
2020年 | 10篇 |
2019年 | 15篇 |
2018年 | 69篇 |
2017年 | 60篇 |
2016年 | 62篇 |
2015年 | 49篇 |
2014年 | 68篇 |
2013年 | 75篇 |
2012年 | 95篇 |
2011年 | 99篇 |
2010年 | 64篇 |
2009年 | 42篇 |
2008年 | 40篇 |
2007年 | 67篇 |
2006年 | 54篇 |
2005年 | 52篇 |
2004年 | 54篇 |
2003年 | 43篇 |
2002年 | 30篇 |
2001年 | 25篇 |
2000年 | 11篇 |
1999年 | 16篇 |
1998年 | 8篇 |
1997年 | 6篇 |
1996年 | 7篇 |
1995年 | 3篇 |
1994年 | 5篇 |
1993年 | 1篇 |
1992年 | 4篇 |
1991年 | 7篇 |
1990年 | 4篇 |
1989年 | 4篇 |
1988年 | 4篇 |
1987年 | 4篇 |
1986年 | 1篇 |
1985年 | 6篇 |
1984年 | 3篇 |
1983年 | 2篇 |
1982年 | 2篇 |
1981年 | 3篇 |
1980年 | 2篇 |
1979年 | 3篇 |
1978年 | 4篇 |
1977年 | 3篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1972年 | 1篇 |
排序方式: 共有1212条查询结果,搜索用时 15 毫秒
151.
152.
Iwona Winiecka Paweł Jaworski Aleksander Paweł Mazurek Dorota Marszałek Anna Goldnik Daniel Sokulski 《Journal of peptide science》2016,22(2):106-115
In search for new drugs lowering arterial blood pressure, which could be applied in anti‐hypertensive therapy, research concerning agents blocking of renin‐angiotensin‐aldosteron system has been conducted. Despite many years of research conducted at many research centers around the world, aliskiren is the only one renin inhibitor, which is used up to now. Four novel potential renin inhibitors, having structure based on the peptide fragment 8–13 of human angiotensinogen, a natural substrate for renin, were designed and synthesized. All these inhibitors contain unnatural moieties that are derivatives of N‐methylleucyl‐β‐hydroxy‐γ‐amino acids at the P2‐P1' position: 4‐[N‐(N‐methylleucyl)‐amino]‐3‐hydroxy‐7‐(3‐nitroguanidino)‐heptanoic acid (AHGHA), 4‐[N‐(N‐methylleucyl)‐amino]‐3‐hydroxy‐5‐phenyl‐pentanoic acid (AHPPA) or 4‐[N‐(N‐methylleucyl)‐amino]‐8‐benzyloxycarbonylamino‐3‐hydroxyoctanoic acid (AAHOA). The previously listed synthetic β‐hydroxy‐γ‐amino acids constitute pseudodipeptidic units that correspond to the P1‐P1' position of the inhibitor molecule. An unnatural amino acid, 4‐methoxyphenylalanin (Phe(4‐OMe)), was introduced at the P3 position of the obtained compounds. Three of these compounds contain isoamylamide of 6‐aminohexanoic acid (ε‐Ahx‐Iaa) at the P2'‐P3' position. The proposed modifications of the selected human angiotensinogen fragment are intended to increase bioactivity, bioavailability, and stability of the inhibitor molecule in body fluids and tissues. The inhibitor Boc‐Phe(4‐OMe)‐MeLeu‐AHGHA‐OEt was obtained in the form of an ethyl ester. The hydrophobicity coefficient, expressed as log P varied between 3.95 and 8.17. In vitro renin inhibitory activity of all obtained compounds was contained within the range 10?6‐10?9 M. The compound Boc‐Phe(4‐OMe)‐MeLeu‐AHPPA‐Ahx‐Iaa proved to be the most active (IC50 = 1.05 × 10?9 M). The compounds Boc‐Phe(4‐OMe)‐MeLeu‐AHGHA‐Ahx‐Iaa and Boc‐Phe(4‐OMe)‐MeLeu‐AHPPA‐Ahx‐Iaa are resistant to chymotrypsin. Copyright © 2016 European Peptide Society and John Wiley & Sons, Ltd. 相似文献
153.
Agnieszka Hernik Paweł Struciński Brian T. Buckley Katarzyna Góralczyk Katarzyna Czaja Wojciech Korcz 《人类与生态风险评估》2016,22(7):1456-1468
The long-term health threats posed to humans exposed to pollutants acting as endocrine disruptors (EDs) is yet to be quantified. There is insufficient knowledge about the sources and magnitude of exposure to selected polybrominated diphenyl ethers (PBDEs) and polychlorinated biphenyls (PCBs) during the most sensitive period of fetal development, suggesting the need for a study. Organochlorine pesticides, classified as being persistent organic pollutants (POPs) and potential EDs, were also included in this analysis. Xenobiotics were measured in paired fetal cord blood and maternal breast milk samples. There were no significant differences in the concentrations of PCB-101, PBDE-47, and PBDE-99 between maternal milk and cord blood according to the Wilcoxon test, and the Spearman tests demonstrated significant correlations in β-HCH, γ-HCH, p,p'-DDE, and PCB-118 between maternal milk and cord blood from the same subjects. All others tested (HCB, β-HCH, γ-HCH, p,p'-DDE, p,p'-DDD, p,p'-DDT, PCB-101, PCB-138, PCB-153, PCB-170, PCB-180, PBDE-153) demonstrated significant differences in the same subject women with concentrations significantly higher in maternal milk than in cord blood. The presence of these compounds found in cord blood and maternal milk indicates that both are a source of perinatal exposure to these pollutants. This study opens up the opportunity for new research in estimating a prenatal exposure based on breast milk concentrations of organohalogen compounds. 相似文献
154.
Jan Vondrák Jiří Malíček Zdeněk Palice Brian Coppins Martin Kukwa Paweł Czarnota Neil Sanderson Andy Acton 《Nordic Journal of Botany》2016,34(5):619-626
We tested two methods to obtain more complete species inventories in surveys of lichen biodiversity. The first was to employ eight lichenologists (all experienced, some specialists) acting as individuals in parallel in a competitive survey. The second was to organize those lichenologists into two competing teams. We show that overall recorded biodiversity is distinctly higher than the part of lichen biodiversity recorded by each single lichenologist (45–66%) or team (79–83%). Use of these methods in a survey of epiphytic and epixylic lichens resulted in a list containing 112 species in 1 ha, 192 species in 12.5 ha and 212 species for 30 km2 of lowland floodplain old‐growth forest in southeastern Czech Republic. Eleven recorded species are new to the country; four are rediscovered after more than 50 years. In comparison, few previous surveys of mixed montane forests in the same region have yielded more than 200 species, even though it is certain that those forests have greater lichen diversity than our lowland forest. 相似文献
155.
156.
Izabela Kołodziejczyk Katarzyna Dzitko Rafał Szewczyk Małgorzata M. Posmyk 《Acta Physiologiae Plantarum》2016,38(6):146
Melatonin (N-acetyl-5-methoxytryptamine) has a great potential for plant biostimulation. Its role in plant physiology is intensively explored, and its important function in plant stress defence frequently underlined. Melatonin is particularly effective when applied as an additional factor of seed priming. In the presented research, hydroconditioning was chosen experimentally as optimal for maize (Zea mays L.) seeds. The following seed variants were compared: controlled non-treated, hydroprimed with water and hydroprimed with melatonin solutions 50 and 500 μM. To identify modifications in proteome of maize seeds caused by the applied hydroconditioning techniques, protein extracts of germinated seed embryos (24 h, 25 °C) were separated by 2D-PAGE. Next, obtained maps of proteomes were compared (statistically and graphically) using PDQuest software, and characteristic spots of proteins were analysed qualitatively by mass-spectrometric techniques and identified in the Mascot protein databases. Research helped to identify hydropriming-associated proteins, and for the first time those which were expressed only in the presence of melatonin. Study confirmed that suitably selected pre-sowing treatment with melatonin, by embryo proteome modification, effectively prepares plants to adverse environmental conditions. In melatonin treated seeds during the initial state of embryos growth, even under optimal conditions, additional antioxidative, detoxifying, anti-stresses proteins were synthesized. Moreover, the supply of energy from seed storage substances was pretty intensified. The presented results partially explain how melatonin acts in plant stress defence, and why plants with higher melatonin content have exhibited increased capacity for stress tolerance. 相似文献
157.
Paweł Adamski Adam Bohdan Jakub Michalcewicz Michał Ciach Zbigniew Witkowski 《Journal of Insect Conservation》2016,20(6):1099-1105
Ecological traps are serious, anthropogenic threats to animal populations. However, in certain cases it is difficult to determine whether they really act in the expected manner. This applies to the harmful effects of beech timber stacked in forests on the endangered saproxylic beetle Rosalia longicorn Rosalia alpina, which have been mentioned in numerous scientific articles, conservation action plans and similar publications. The aim of this paper is to determine whether beech timber stacks meet the criteria of an ecological trap for the Rosalia longicorn. Two basic criteria of such a trap are analysed: the attractiveness of timber stacks and the impossibility of complete larval development. The results show that beech timber stacks are highly attractive to Rosalia longicorn imagines. Moreover, the time during which the timber is stacked is shown to be significantly shorter than the species’ larval development period. These results suggest that timber stacks can be treated as operative ecological traps for the Rosalia longicorn, even though the extent of their influence on the demographic parameters of this beetle’s population has not been estimated. Forest management practices, i.e. increasing amounts and shifts in timing of wood storage, could intensify this threat. 相似文献
158.
Joanna Kajzer-Bonk Damian Szpiłyk Michał Woyciechowski 《Journal of Insect Conservation》2016,20(1):99-105
Goldenrods (Solidago sp.) are currently one of the most invasive plant species in Central Europe. They threaten abandoned semi-natural wet grasslands which are extremely vulnerable to plant succession and invasions. We assessed whether Solidago invasion affects ants, keystone organisms essential to proper ecosystem functioning and to the existence of myrmecophilous Phengaris butterflies. Ten meadows containing 60 plots with and without goldenrods were studied. We found a strong, negative dependence between the presence of goldenrod cover and the number of ant nests (more than 50 % reduction compared to control) as well as the number of species, and changes in species composition. Myrmica ants, essential hosts for Phengaris larvae, were among the most affected species by goldenrod invasion. Immediate action should be undertaken for restoration and maintenance of biodiversity hotspots affected by goldenrod invasion. 相似文献
159.
160.
Anti-cancer activity of targeted pro-apoptotic peptides. 总被引:21,自引:0,他引:21
H M Ellerby W Arap L M Ellerby R Kain R Andrusiak G D Rio S Krajewski C R Lombardo R Rao E Ruoslahti D E Bredesen R Pasqualini 《Nature medicine》1999,5(9):1032-1038
We have designed short peptides composed of two functional domains, one a tumor blood vessel 'homing' motif and the other a programmed cell death-inducing sequence, and synthesized them by simple peptide chemistry. The 'homing' domain was designed to guide the peptide to targeted cells and allow its internalization. The pro-apoptotic domain was designed to be nontoxic outside cells, but toxic when internalized into targeted cells by the disruption of mitochondrial membranes. Although our prototypes contain only 21 and 26 residues, they were selectively toxic to angiogenic endothelial cells and showed anti-cancer activity in mice. This approach may yield new therapeutic agents. 相似文献