The toxic influence of silver and titanium dioxide nanoparticles on cultured ovarian granulosa cells

The application of metal nanoparticles in modern society is growing, but there is insufficient data concerning their influence on reproductive processes and comparison of their biological activity. The present experiments aimed to compare the effects of silver and titanium dioxide nanoparticles (AgNPs and TiO2NPs) on ovarian granulosa cell functions. AgNPs and TiO2NPs were added to culture of porcine granulosa cells at doses 0, 0.01, 0.1, 1 or 10 μg/mL. The mRNAs for proliferating cell nuclear antigen (PCNA), cyclin B1, bax and caspase 3 were quantified by RT-PCR; release of progesterone was analyzed by ELISA. It was shown that both AgNPs and TiO2NPs significantly reduced all the measured parameters. ED₅₀ of the inhibitory influence of AgNPs on the main ovarian cell parameters was higher than ED₅₀ of TiO2NPs. The ability of AgNPs and TiO2NPs to suppress ovarian granulosa cell functions should be taken into account by their application.     

Effect of experimental top soil removal on vegetation of Pannonian salt steppes

Inland saline habitats of the Pannonian Lowland exhibit a specific variety of grasslands determined by a soil salinity gradient. Changes in the hydrological regime and absence of management have resulted in heavy degradation of the vegetation. The impact of topsoil removal on salt steppes was tested by a 3-year small-scale manipulated experiment in SW Slovakia (Kamenínske Slanisko Nature Reserve). Topsoil was removed in three contrasting types of vegetation with different soil salinities, i.e. in different stages of habitat degradation. Data were analysed by multi-way ANOVA and by multivariate methods. Species richness decreased and the proportion of halophytes increased significantly in the two types with the highest soil salinity; however, the total number of halophytes was not influenced by soil removal. The treatment caused inhibition of secondary succession on the plots with the highest salinity. The effect of the soil removal was only short-term in the vegetation with moderate salinity and on heavily degraded and desalinized types it even stimulated further recruitment of ruderal species. Topsoil removal has only limited potential for the restoration of Pannonian salt steppes. It should be applied only in slightly degraded vegetation, where salt accumulation is still present and target species propagules are available.     

Growth models of cultures with two liquid phases. 8. Experimental observations on droplet size and interfacial area

Because of the importance of the drop she distribution and interfacial area of the dispersed liquid phase in hydrocarbon fermentations, experiments were carried out to determine the drop size distribution and the interfacial area during batch fermentations of Candida lipolytica on gas oil and on n-hexadecane dissolved in dewaxed gas oil. The effects of cell concentration and dispersed phase volume fraction on size distribution and interfacial area were investigated. Measurements of interfacial tensions, densities, viscosities, and fatty acid concentrations were also made. The results show that the size distribution is skewed and that the Sauter mean diameter is in the range of 10 to 30 μ. Both the Sauter mean diameter and the interfacial area increased during the course of a batch fermentation; however, they decreased at the end of the fermentation. The interfacial area also increased with inoculum size.     

Within-crown pattern of ecophysiological features in leaves ofAcer campestre andCarpinus betulus

The crowns of mature sample trees ofAcer campstre L. andCarpinus betulus L. growing in a closed forest served for the determination of water content in leaves, of specific leaf area and stomatal density. Three layers were taken into account, the position of the leaf with regard to the exposure of the particular portion of the crown, leaf insertion within the limb, and changes in the course of the growing period.