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51.
52.
O. T. Titarenko M. E. Dyakova D. S. Esmedlyaeva M. V. Pavlova A. V. Yelkin N. P. Alekseeva B. B. Bondarenko 《Biochemistry (Moscow) Supplemental Series B: Biomedical Chemistry》2011,5(3):301-306
Functional activity of circulating phagocytes (macrophages and neutrophils — Ns) was studied in 30 patients with infiltrative
and 30 patients with fibro-cavernous pulmonary tuberculosis (IPT and FCPT, respectively), characterized by similar biological
properties of mycobacterium tuberculosis (MTB), dissemination of the process, and manifestations of intoxication. Differences
of the functional activity of both types of cells depending on the PT form were found: a more significant increase in the
oxygen-depending activity in FCPT while bactericide potential estimated with the zymosan induced NST-test was more pronounced
in IPT patients. These data correlate with the blood levels of neopterin and elastase, the markers of the mononuclear and
neutrophil activity, respectively. Involvement of adenosine deaminase (ADA) and neopterin in realization of intracellular
oxygen-dependent processes was demonstrated. Results of the multivariate analysis of the whole set of the studied phagocyte
parameters, reflecting their different roles in this pathological process demonstrated a prevailing role of mononuclears in
newly diagnosed IPT and neutrophils in the chronic progressive process. 相似文献
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54.
Platikanov S Nikolov S Pavlova D Evstatieva L Popov S 《Zeitschrift für Naturforschung. C, Journal of biosciences》2005,60(7-8):591-599
This paper shows the changes of the volatile compounds from four Astragalus species at three phenological stages: leaf development, flowering and fructification, which might be connected with the plant defense. After GC/MS analyses of Astragalus glycyphyllos L., A. hamosus L., A. cicer L. and A. spruneri Boiss., different groups of volatile compounds were found: hydrocarbons, alcohols, aldehydes and ketones, esters, terpenes, chlorinated compounds, etc. Identified volatiles were used for a cluster analysis in order to make chemotaxonomic conclusions for these evolutionary different species. 相似文献
55.
56.
Pavlova IV Vanetsian GL 《Rossi?skii fiziologicheski? zhurnal imeni I.M. Sechenova / Rossi?skaia akademiia nauk》2006,92(11):1273-1284
By plotting autocorrelation histograms, the character of impulsation of neurons in bilateral derivations from cortical visual and somatosensory areas and hippocampal field CA1 was studied in rabbits during free behavior under the influences of emotional stimuli. During active orienting-exploratory reaction of rabbits, most cortical and hippocampal neurons manifested bursting discharges and theta-frequency oscillation (predominantly 4-5 Hz in cortex and 4-5, 6-7 in hyppocampus). During freezing, as compared with active locomotor reaction, the number of neurons with equiprobabilistic discharges increased; in impulsation of neurons with periodicity, the intensity of delta-frequency oscillation increased (predominantly 2-4 Hz) but theta-frequency oscillation decreased. The number of neurons with delta-frequency oscillation during freezing was greater then in calmly sitting rabbits. 相似文献
57.
The gene encoding mouse single chain antibody (ScFv) against human interferon alpha2b (IFN-alpha2b) was cloned into the plasmid vector under the control of promoter from phage T7 and the recombinant protein was expressed in Escherichia coli as inclusion bodies. After the isolation of inclusion bodies the desired protein containing affinity tail "6His tag" was solubilized and purified under denaturing conditions by immobilized-metal affinity chromatography. The soluble and purified ScFv was obtained by "on column" refolding and the recovery of biological activity were demonstrated. The higher levels of ScFv production for intracellular expression system in comparison with ScFv obtained by secretion were shown. The advantages of described refolding method are simplicity and high efficacy, moreover, refolding using a chromatographic process represents the manufacturable approach because it is easily automated using commercially available materials and preparative chromatography systems and also can be combined with simultaneous purification. 相似文献
58.
Pavlova IV Levshina IP Vanetsian GL Pavlov IuV Shuĭkin NN Ziablitseva EA 《Zhurnal vysshe? nervno? deiatelnosti imeni I P Pavlova》2006,56(1):65-74
Different behavioral reactivity of rabbit groups differentiated by locomotor activity in the "open field" was revealed during exposure to emotional stimuli (rustle, loud sound, pressuring on back of the neck, vibroacoustic tactile stimulation of an ear). In passive rabbits, the active locomotor reactions were induced harder and freezing was obtained easier than in active animals. During exposure to sound stimuli, passive rabbits increased their locomotion more rarely than active animals, pressing on back of the neck produced longer freezing, a threshold of defensive ear shaking in response to a vibroacoustic stimulus in passive animals was highest. Training to mild immobilization increased the threshold of defensive responses in active rabbits and animals of the intermediate type. Changes in respiratory parameters were correlated with behavioral reactions to emotional stimuli. The duration of exhalation and respiratory cycle increased during freezing and increased during enhanced locomotion. The duration of inhalation decreased in response to emotional stimuli irrespective of a behavioral reaction. The respiratory reactions to emotional stimuli differed in rabbits of different groups. The respiratory rate more frequently changed in passive rabbits than in animals of other groups. Passive animals reacted mainly by exhalation, active rabbits and animals from the intermediate group predominantly responded by inhalation. 相似文献
59.
Atanassova N Koeva Y Bakalska M Pavlova E Nikolov B Davidoff M 《Folia histochemica et cytobiologica / Polish Academy of Sciences, Polish Histochemical and Cytochemical Society》2006,44(2):81-86
Androgens are especially important for the maintenance of spermatogenesis in adulthood and the experimental withdrawal of testosterone (T) production by ethane dimenthanesulfonate (EDS) is a valuable tool for studying androgen-dependent events of spermatogenesis. The aim of the present study was to investigate the specific changes in immunoexpression of androgen receptor (AR) in the testis in relation to degeneration and regeneration of Leydig cell (LC) population and seminiferous epithelium. Immunohistochemistry for AR and 3beta-hydroxysteroid dehydrogenase (3beta-HSD) as well as TUNEL assay for apoptosis were performed on testicular sections of control and EDS-treated rats. Serum LH and T levels were measured by RIA. Our results revealed a total loss of AR immunoexpression from the nuclei of Sertoli (SCs), LCs and peritubular cells during the first week after EDS administration and that coincided with severe drop in T levels. Two weeks after EDS administration, the AR expression was recovered in these cells but normal stage-specificity in SCs was replaced by uniform intensity of AR immunostaining at all the stages of the spermatogenic cycle. The stage-specific pattern of androgen expression in SCs with a maximum at stages VII-VIII appeared 5 weeks after treatment. LC immunoreactivity for 3beta-HSD at different time points after EDS administration correlated with values of T concentration. The maximal germ cell apoptosis on day 7 was followed by total loss of elongated spermatids 2 weeks after EDS treatment. Regeneration of seminiferous epithelium 3 weeks after EDS administration and onwards occurred in tandem with the development of new LC population indicated by the appearance of 3beta-HSD-positive cells and gradual increase in T production. The specific changes in AR after EDS including their loss and recovery in Sertoli cells paralleled with degenerative and regenerative events in Leydig and germ cell populations, confirming close functional relationship between Sertoli, Leydig and germ cells. 相似文献
60.
Petrenko AA Pavlova LS Karseladze AI Kisseljov FL Kisseljova NP 《Biochemistry. Biokhimii?a》2006,71(10):1153-1160
We explored the expression of four genes encoding for subunits of AP-3 in cervical tumors and cancer cell lines. Using RT-PCR we demonstrated more than twofold decrease in the levels of mRNA of AP3D1, AP3B1, AP3M1, and AP3S1 in 32, 28, 23, and 26% tumors in comparison with normal tissues of uterine cervix, respectively. The level of mRNA of at least one subunit was decreased in 28 out of 47 (60%) of tumors and in four out of five cancer cell lines in comparison to tissues adjacent to tumors. The suppression of expression of any of the subunits was revealed in 15 out of 28 cases (54%). The expression of two and more subunits was decreased simultaneously in different combinations in 13 cases (46%). This fact testifies to the lack of a common mechanism of downregulation of four subunits in tumors. There is a tendency to more frequent suppression of AP-3A expression in tumors associated with lymphatic node metastases as compared with tumors without metastases (P = 0.034). Thus, here we demonstrate for the first time the decrease in expression of genes encoding for AP-3A subunits in tumors. 相似文献