全文获取类型
收费全文 | 298篇 |
免费 | 29篇 |
国内免费 | 1篇 |
出版年
2023年 | 3篇 |
2022年 | 3篇 |
2021年 | 4篇 |
2019年 | 4篇 |
2018年 | 7篇 |
2017年 | 3篇 |
2016年 | 5篇 |
2015年 | 16篇 |
2014年 | 8篇 |
2013年 | 16篇 |
2012年 | 20篇 |
2011年 | 13篇 |
2010年 | 18篇 |
2009年 | 15篇 |
2008年 | 10篇 |
2007年 | 12篇 |
2006年 | 11篇 |
2005年 | 9篇 |
2004年 | 11篇 |
2003年 | 5篇 |
2002年 | 7篇 |
2001年 | 5篇 |
2000年 | 7篇 |
1999年 | 8篇 |
1997年 | 3篇 |
1996年 | 2篇 |
1995年 | 3篇 |
1993年 | 3篇 |
1991年 | 3篇 |
1990年 | 3篇 |
1989年 | 5篇 |
1988年 | 9篇 |
1987年 | 3篇 |
1986年 | 5篇 |
1985年 | 2篇 |
1984年 | 9篇 |
1983年 | 5篇 |
1980年 | 2篇 |
1978年 | 3篇 |
1977年 | 2篇 |
1975年 | 3篇 |
1974年 | 5篇 |
1973年 | 5篇 |
1972年 | 4篇 |
1969年 | 2篇 |
1968年 | 2篇 |
1967年 | 2篇 |
1966年 | 4篇 |
1964年 | 2篇 |
1960年 | 2篇 |
排序方式: 共有328条查询结果,搜索用时 15 毫秒
61.
Heroen Verbruggen † Lennert Tyberghein † Klaas Pauly † Caroline Vlaeminck Katrien Van Nieuwenhuyze Wiebe H.C.F. Kooistra Frederik Leliaert Olivier De Clerck 《Global Ecology and Biogeography》2009,18(4):393-405
Aim Because of their broad distribution in geographical and ecological dimensions, seaweeds (marine macroalgae) offer great potential as models for marine biogeographical inquiry and exploration of the interface between macroecology and macroevolution. This study aims to characterize evolutionary niche dynamics in the common green seaweed genus Halimeda, use the observed insights to gain understanding of the biogeographical history of the genus and predict habitats that can be targeted for the discovery of species of special biogeographical interest. Location Tropical and subtropical coastal waters. Methods The evolutionary history of the genus is characterized using molecular phylogenetics and relaxed molecular clock analysis. Niche modelling is carried out with maximum entropy techniques and uses macroecological data derived from global satellite imagery. Evolutionary niche dynamics are inferred through application of ancestral character state estimation. Results A nearly comprehensive molecular phylogeny of the genus was inferred from a six‐locus dataset. Macroecological niche models showed that species distribution ranges are considerably smaller than their potential ranges. We show strong phylogenetic signal in various macroecological niche features. Main conclusions The evolution of Halimeda is characterized by conservatism for tropical, nutrient‐depleted habitats, yet one section of the genus managed to invade colder habitats multiple times independently. Niche models indicate that the restricted geographical ranges of Halimeda species are not due to habitat unsuitability, strengthening the case for dispersal limitation. Niche models identified hotspots of habitat suitability of Caribbean species in the eastern Pacific Ocean. We propose that these hotspots be targeted for discovery of new species separated from their Caribbean siblings since the Pliocene rise of the Central American Isthmus. 相似文献
62.
M. Ardhaoui A. Falcimaigne J. M. Engasser P. Moussou G. Pauly M. Ghoul 《Biocatalysis and Biotransformation》2004,22(4):253-259
Enzymatic acylation of rutin and esculin with aromatic, aliphatic and aryl-aliphatic acids using Candida antarctica lipase in tert amyl alcohol as solvent was investigated under low water content. Whatever the acyl donor used, the conversion yields and initial rates for esculin were higher than for rutin. For a given flavonoid, the performance of these reactions depended on the acyl donor structures. For aliphatic acids, conversion yields and initial rates of both flavonoids were respectively in the ranges of 68-90% and of 9.5×10-2-72×10-2 mmol l-1 h-1. For aromatic acids, the reaction occurred only with the aryl subgroup (cinnamic, hydrocinnamic, 3,4-dihydroxyhydrocinnamic and 4-hydroxyphenyl acetic acids) and was drastically influenced by the presence of side chain and substitution patterns of the aromatic ring. Except for hydrocinnamic acid (75%, 23.4×10-2 mmol l-1 h-1), with these acids the conversion yields and initial rates were lower and in the range of 10-45% and of 0.7×10-2 to 12.1×10-2 mmol l-1 h-1. Unsaturation of the side chain of the hydrocinnamic acid decreased the esculin conversion rate from 75 to 13% and initial rate from 23.4 to 1.76×10-2 mmol l-1 h-1. The presence of hydroxyl or nitro-groups on the aromatic ring of the aryl aliphatic acid also reduced conversion yields and initial rates. Even without a spacer, the non-phenolic ring acid (quinic acid) was reactive and lead to conversion yields of about 20 and 23% respectively for rutin and esculin. 相似文献
63.
64.
D. Pauly J. Moreau Ma. Lourdes D. Palomares 《Zeitschrift fur angewandte Ichthyologie》1988,4(4):190-193
The growth, standing stocks and production of tilapia Sarotherodon melanotheron (Cichlidae) in the 1 km2 Sakumo Lagoon near Tema, Ghana, are estimated by application of recently developed models to previously published data. The new estimates are used to obtain values of food (i.e. sediment) consumption, some related statistics, and to assess bioturbation as caused by S. melanotheron.
Detritus, Energieverbrauch und Konversionseffizienz von Sarotherodon melanotheron (Cichlidae) in einer westafrikanischen Lagune
Das Wachstum, die Biomasse und die Produktion von Tilapia Sarotherodon melanotheron (Cichlidae) in der 1 km2 Sakumo Lagune bei Tema, Ghana, wurden bestimmt auf der Basis von neueren Modellen und bereits veröffentlichten Daten. Die neuen Modellparameter werden benutzt, um die Nahrungs-, d. h. Sedimentkonsumption und verwandte Größen zu bestimmen, und um die Bioturbation zu schätzen, die S. melanotheron verursacht.
Détritus, dépense d'énergie et efficience de la conversion de Sarotherodon melanotheron (cichlidés) dans une lagune en Afrique de l'ouest
La croissance, la biomasse et la production du tilapia Sarotherodon melanotheron (Cichlidés) ont été estimées pour le stock de la lagune de Sakumo (1 km2 ), près de Tema, Ghana, en utilisant des modèles recents et des données déjà publiées. Les nouvelles valeurs sont utilisées pour l'estimation de la consommation en nour re (= sediment) ainsi que quelques autres charactéristiques de S. melanotheron , et pour estimer le taux de bioturbation qui résulte de ses activités. 相似文献
Zusammenfassung
Detritus, Energieverbrauch und Konversionseffizienz von Sarotherodon melanotheron (Cichlidae) in einer westafrikanischen Lagune
Das Wachstum, die Biomasse und die Produktion von Tilapia Sarotherodon melanotheron (Cichlidae) in der 1 km
Résumé
Détritus, dépense d'énergie et efficience de la conversion de Sarotherodon melanotheron (cichlidés) dans une lagune en Afrique de l'ouest
La croissance, la biomasse et la production du tilapia Sarotherodon melanotheron (Cichlidés) ont été estimées pour le stock de la lagune de Sakumo (1 km
65.
A M Bobst S C Kao E V Bobst G T Pauly 《Journal of biomolecular structure & dynamics》1985,3(2):249-260
Stoichiometric amounts of poly-L-lysine were added to site-specifically spin labeled single stranded nucleic acids and the resulting complexes analyzed by electron spin resonance spectroscopy (ESR). The nucleic acids were spin labeled to different extents and with labels of varying tether length. The ESR data are used to determine nucleoside dynamics and some structural features in these complexes. It is concluded that two distinct base mobilities exist in the complexes; one set is characterized by a mean correlation time tau -R = 2 ns, and the other one by a tau -R greater than or equal to 50 ns. A model is proposed which suggests that a poly-L-lys single stranded nucleic acid complex consists of low mobility segments flanked by more mobile bases. An interesting feature of the proposed model is its applicability to explain ESR data of single strand binding protein-spin labeled nucleic acid complexes, which can also be interpreted in terms of two distinct nucleoside mobility states. It is hypothesized that this phenomenon could be of biological significance for the release of protein ligands from a protein-nucleic acid complex. 相似文献
66.
One of the main expected responses of marine fishes to ocean warming is decrease in body size, as supported by evidence from empirical data and theoretical modeling. The theoretical underpinning for fish shrinking is that the oxygen supply to large fish size cannot be met by their gills, whose surface area cannot keep up with the oxygen demand by their three‐dimensional bodies. However, Lefevre et al. (Global Change Biology, 2017, 23, 3449–3459) argue against such theory. Here, we re‐assert, with the Gill‐Oxygen Limitation Theory (GOLT), that gills, which must retain the properties of open surfaces because their growth, even while hyperallometric, cannot keep up with the demand of growing three‐dimensional bodies. Also, we show that a wide range of biological features of fish and other water‐breathing organisms can be understood when gill area limitation is used as an explanation. We also note that an alternative to GOLT, offering a more parsimonious explanation for these features of water‐breathers has not been proposed. Available empirical evidence corroborates predictions of decrease in body sizes under ocean warming based on GOLT, with the magnitude of the predicted change increases when using more species‐specific parameter values of metabolic scaling. 相似文献
67.
68.
The impedance of erythrocytes of man, cattle, sheep, dog, cat, rabbit, and chicken was measured in the range from 0.5 to 250 Mc. The dielectric constant of the red cell interior is 50 at 250 Mc, varies but little with species, and can readily be accounted for by the cells' hemoglobin content. The electrical conductivity of the red cell interior was determined between 70 and 100 Mc. The values differ from species to species within the rather limited range from 4.4 to 5.3 mmho/cm. Removal of the cell membranes does not affect the conductivity. Hence, the cell interior behaves, from an electrical point of view, like a highly concentrated hemoglobin solution. A theoretical value for the electrical conductivity of erythrocyte interiors, which is calculated on the basis of the salt content of the cell, ion mobility, and the volume concentration of the hemoglobin, is roughly twice as large as the measured value. This discrepancy is typical not only of the red blood cell. Pertinent measurements show that it is probably caused by hydrodynamic and possibly by electrostatic effects also, which lower the mobility of the ions. From the lower electrical mobility it appears that a lowered diffusion constant of the electrolytes and nonelectrolytes within the cell is indicated. 相似文献
69.
G Egert F P Thinnes H G?tz E Pauly H Kratzin C Y Yang S K?lbel P Altevogt P Wernet N Hilschmann 《Hoppe-Seyler's Zeitschrift für physiologische Chemie》1984,365(11):1291-1308
In a previous communication we described the preparation of the alpha/beta-chain complex of HLA-D membrane antigens from a homozygous lymphoblastoid B cell line, H2LCL (HLA-A3,3; B7,7; Dw2,2; DR2,2; MT1,1; DC1,1; MB1,1). In this paper we present the separation of the alpha- and beta-chains in quantitative scale using hydroxylapatite chromatography with a pH 7.15 phosphate buffer, containing sodium dodecyl sulfate. An alternative isolation procedure is electrophoresis, taking advantage of the different isoelectric points of the alpha- and beta-chains. The relative molecular masses, the isoelectric points and the N-terminal sequences are discussed and compared with the results of other investigators. Remarkable is the polymorphism of both the chains, especially the beta-chains. The importance of the previously described three step purification procedure for the preparation of these antigens for sequence studies has been pointed out. 相似文献
70.
Summary Control of mitochondrial respiration depends on ADP availability to the F1ATPase. An electrochemical gradient of ADP and ATP across the mitochondrial inner membrane is maintained by the adenine nucleotide translocase which provides ADP to the matrix for ATP synthesis and ATP for energy-dependent processes in the cytosol. Mitochondrial respiration is responsive to the cytosolic phosphorylation potential, ATP/ADP · Pi which is in apparent equilibrium with the first two sites in the electron transport chain. Conventional measures of free adenine nucleotides is a confounding issue in determining cytosolic and mitochondrial phosphorylation potentials. The advent of phosphorus-31 nuclear magnetic resonance (P-31 NMR) allows the determination of intracellular free concentrations of ATP, creatine-P and Pi in perfused muscle in situ. In the glucose-perfused heart, there is an absence of correlation between the cytosolic phosphorylation potential as determined by P-31 NMR and cardiac oxygen consumption over a range of work loads. These data suggest that contractile work leads to increased generation of mitochondrial NADH so that ATP production keeps pace with myosin ATPase activity. The mechanism of increased ATP synthesis is referred to as stimulusre-sponse-metabolism coupling. In muscle, increased contractility is a result of interventions which increase cytosolic free Ca2+ concentrations. The Ca2- signal thus generated increases glycogen breakdown and myosin ATPase in the cytosol. This signal is concomitantly transmitted to the mitochondria which respond to small increases in matrix Ca2+ by activation of Ca2+-sensitive dehydrogenases. The Ca2+-activated dehydrogenase activities are key rate-controlling enzymes in tricarboxylic acid cycle flux, and their activation by Ca2- leads to increased pyridine nucleotide reduction and oxidative phosphorylation. These observations which have been consistent in preparations both in vitro and in situ do not obviate a role for ADP control of muscle respiration, but do explain, in part, the lack of dramatic fluctuations in the cytosolic phosphorylation potential over a large range of contractile activities. 相似文献