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91.
Ana Paula Souza Caetano Daniela Guimarães Simão Renata Carmo-Oliveira Paulo Eugênio Oliveira 《Plant Systematics and Evolution》2013,299(7):1253-1262
Apomixis, or asexual reproduction through seeds, has been reported for species of the tribe Miconieae, Melastomataceae, but details of the process have yet to be described. We analyzed and compared sporogenesis and gametogenesis in the apomictic Miconia albicans and the sexual M. chamissois. The results point to some differences between species, which were related to the apomictic process. In M. albicans microsporogenesis, problems during meiosis and degeneration of its products led to total pollen sterility, while M. chamissois presented normal bicellular pollen grains in the mature anther. The absence or abnormality of meiosis in M. albicans megasporogenesis led to the formation of an unreduced embryo sac and also to egg cell parthenogenesis, which gave rise to the apomictic embryo. Embryo and endosperm development were autonomous, resulting in seeds and fruits independent of pollination and fertilization. Thus, in this species, apomixis can be classified as diplosporic and obligate. In contrast, meiosis was as expected in the sexual M. chamissois, and led to the development of a reduced embryo sac. Despite the divergent pathways, many embryological characteristics were similar between the studied species and other Melastomataceae and they seem to be conservative character states for the family. 相似文献
92.
Mercedes di Pasquo George W. Grader Peter Isaacson Paulo A. Souza Roberto Iannuzzi Enrique Díaz-Martínez 《Historical Biology》2013,25(7):868-897
This paper presents new data about Early Permian (Cisuralian) strata, palynostratigraphy and absolute dating from the Copacabana Formation in central Bolivia. Recent stratigraphic and palynologic data from marine and transitional rocks at Apillapampa refine the age of Cisuralian palynomorphs in South America. Twelve samples interbedded with five volcanic ashes (processed and productive) yielded 94 palynomorph species arranged in two informal palynoassemblages: the lower assemblage Vittatina costabilis corresponds to one sample near the base of the Copacabana Formation and the upper Lueckisporites virkkiae assemblage occurs in overlying marine and coal-bearing transitional intervals. Ages were also independently refined by a modern review of conodonts, fusulinids, along with those U–Pb radiometric ages (Isotope Dilution Thermal Ionization Mass Spectrometry [ID-TIMS] of zircon-bearing interbedded tuffs). These data suggest that the lower marine member of the Copacabana Formation at this location is Asselian and Sakmarian. Lueckisporites virkkiae is a key species of palynomorph utilised in South American and global Permian biostratigraphic reconstructions. Hence, a thorough global comparison of these palynofloras and correlations is addressed in this contribution, considering first appearances of mainly cosmopolitan diagnostic taxa. Correlations are established with many similar Permian palynofloras, some also constrained with radiometric data, in South America (Brazil, Uruguay and Argentina) and elsewhere. 相似文献
93.
Carolina Loch Warwick Duncan Paulo C. Simões-Lopes Jules A. Kieser R. Ewan Fordyce 《Zoomorphology》2013,132(2):215-225
Longitudinal and cross sections of teeth from 17 species of the Recent dolphins (Delphinoidea and Inioidea) were examined under scanning electron microscope to study the arrangement and ultrastructure of dental tissues with reference to phylogenetic and functional constraints. For most species, enamel had a simple bi-layered structure of radial enamel and an outer layer of prismless enamel. The outer prismless layer varied from 5 to 30 % of enamel thickness. The enamel of Burmeister’s porpoise (Phocoena spinipinnis) was entirely prismless. The prisms had an open sheath; tubules and tuft-like structures were common at the enamel-dentine junction. Cetacean dentine was characterized by irregularly distributed dentinal tubules in a relatively homogenous dentinal matrix. Radial enamel was observed in all Delphinoidea and in the franciscana (Pontoporia blainvillei), whereas the Amazon river dolphin (Inia geoffrensis) had prisms organized in Hunter–Schreger bands. HSB in enamel are regarded as a device for resisting propagation of cracks. These may occur due to increased functional demands, possibly related to the hardness of the species diet. Simplification in tooth shape and reduced biomechanical demands plausibly explain the primitive radial organization among delphinoids and Pontoporia. The HSB structure in the Amazon river dolphin, similar to those of extinct archaeocetes, seems to have secondary functional implications. However, the distribution of HSB in more-basal odontocetes is too poorly known to judge whether the HSB of Inia are a retained plesiomorphic feature or convergence. 相似文献
94.
Rachel S. N. de Pinho Francisco P. da Silva‐Júnior João Paulo C. Bastos Werllen S. Maia Marco Túlio de Mello Veralice M. S. de Bruin 《Chronobiology international》2013,30(5):963-971
Truck drivers are more likely to suffer severe injury and death due to certain truck driving characteristics. Identifying and preventing factors associated with accidents in this population is important to minimize damage and improve road safety. Excessive daytime sleepiness is a major public health problem, leading to impaired cognitive function, reduced alertness, and increased risk of motor vehicle crashes. The aim of this cross‐sectional study was to determine the prevalence and predictors of hypersomnolence (defined as an Epworth Sleepiness Scale score greater than 10) among truck drivers. Three hundred male truck drivers were studied. Quality of sleep was assessed by the Pittsburgh Sleep Quality Index, and the association between demographic, clinical, and occupational data with excessive sleepiness was analyzed. The mean daily sleep duration was 5.6±1.3 h, and poor quality of sleep was found in 46.3% of the individuals. Hypersomnolence was found in 46% of the drivers and was associated with younger age, snoring, and working >10 h without rest. A positive correlation between hypersomnolence and previous accidents was detected (p=0.005). These results show that sleep deprivation and hypersomnolence are frequent among truck drivers. The treatment of sleep‐disordered breathing and the implementation of educational programs, particularly targeting younger drivers and promoting increased awareness of the deleterious effects of sleep loss and work overload, may help to reduce hypersomnolence and accidents among truck drivers. 相似文献
95.
Fabiana Campos Hirata Monica Colares Oliveira Lima Paulo Ribeiro Nóbrega Germano Paulo Wenceslau Pedro Felipe Carvalhedo de Bruin 《Chronobiology international》2013,30(5):939-946
Medical students are at higher risk for depression, affecting not only their lives but also patient care. This article studied a population of medical students engaged in lecture‐based learning regarding the presence of depressive symptoms and its relation to morningness‐eveningness. Depressive symptoms were assessed by the Beck Depressive Inventory scale (BDI>10), and diurnal preference was assessed by the Horne & Östberg Morningness/Eveningness Questionnaire (MEQ). Family history of depression and involvement in regular physical activity were also investigated. A total of 161 students, 77 (47.8%) males, aged 19 to 30 yrs (22.1±2.1) living in a city close to the equator were evaluated. Fifty‐three individuals (32.9%) had depressive symptoms. Depressive individuals showed a trend to be female (p=0.07). Also, female gender showed a non‐significant shift toward morningness. Fifty‐eight (36.0%) subjects participated in regular physical activity. In 57 cases (35.4%), there was a history of depression in the family. Fifteen individuals (9.3%) were definitely evening type, 42 (26.1%) were moderately evening type, 44 (27.3%) were indifferent, 42 (26.1%) were moderately morning type, and 18 (11.2%) were definitely morning type. Family history of depression (OR=0.29, 95% CI=1.37–6.12) and sedentary life (OR=0.28, 95% CI=0.12–0.65) were associated with depressive symptoms. Eveningness was associated with depressive symptoms (OR=0.66, 95% CI=0.50–0.88), and this association remained significant after adjusting for the presence of familial depression and physical activity (OR=0.71, 95% CI=0.52–0.95). In conclusion, depressive symptoms are independently associated with “eveningness” in medical students. These results should be confirmed by future studies involving a larger number of subjects. 相似文献
96.
Rúben F. Pereira Anabela Carvalho M.H. Gil Ausenda Mendes Paulo J. Bártolo 《Carbohydrate polymers》2013
This study investigates the influence of Aloe vera on water absorption and the in vitro degradation rate of Aloe vera-Ca-alginate hydrogel films, for wound healing and drug delivery applications. The influence of A. vera content (5%, 15% and 25%, v/v) on water absorption was evaluated by the incubation of the films into a 0.1 M HCl solution (pH 1.0), acetate buffer (pH 5.5) and simulated body fluid solution (pH 7.4) during 24 h. Results show that the water absorption is significantly higher for films containing high A. vera contents (15% and 25%), while no significant differences are observed between the alginate neat film and the film with 5% of A. vera. The in vitro enzymatic degradation tests indicate that an increase in the A. vera content significantly enhances the degradation rate of the films. Control films, incubated in a simulated body fluid solution without enzymes, are resistant to the hydrolytic degradation, exhibiting reduced weight loss and maintaining its structural integrity. Results also show that the water absorption and the in vitro degradation rate of the films can be tailored by changing the A. vera content. 相似文献
97.
Paulo De Marco Júnior 《Studies on Neotropical Fauna and Environment》2013,48(3):186-189
During quantitative samples of invertebrate fauna in two lakes in the Rio Doce State Park (Marliéria, MG) I found some individuals of the introduced aquatic snail Melanoides tuberculata. It was most abundant in areas with high human activity, where the original vegetation was removed from the shallow areas near the margin. Population structure inferred by size-class data suggests that the most impacted area was invaded first and that these snails maintain a viable population in Dom Helvécio lake. Potential spread of the snail in this lake system is tentatively related to the characteristics of the bank vegetation and I presume that the not yet invaded Carioca lake has better conditions for an exponential growth of this species. 相似文献
98.
Paulo De Sa Peixoto Guillaume Laurent Thierry Aza?s Gervaise Mosser 《The Journal of biological chemistry》2013,288(11):7528-7535
In vivo, collagen I, the major structural protein in human body, is found assembled into fibrils. In the present work, we study a high concentrated collagen sample in its soluble, fibrillar, and denatured states using one and two dimensional {1H}-13C solid-state NMR spectroscopy. We interpret 13C chemical shift variations in terms of dihedral angle conformation changes. Our data show that fibrillogenesis increases the side chain and backbone structural complexity. Nevertheless, only three to five rotameric equilibria are found for each amino acid residue, indicating a relatively low structural heterogeneity of collagen upon fibrillogenesis. Using side chain statistical data, we calculate equilibrium constants for a great number of amino acid residues. Moreover, based on a 13C quantitative spectrum, we estimate the percentage of residues implicated in each equilibrium. Our data indicate that fibril formation greatly affects hydroxyproline and proline prolyl pucker ring conformation. Finally, we discuss the implication of these structural data and propose a model in which the attractive force of fibrillogenesis comes from a structural reorganization of 10 to 15% of the amino acids. These results allow us to further understand the self-assembling process and fibrillar structure of collagen. 相似文献
99.
Nicolás O. Amiano María J. Costa R. Macarena Reiteri Cristian Payés Diego Guerrieri Nancy L. Tateosian Mercedes L. Sánchez Paulo C. Maffia Miriam Diament Romina Karas Andrés Orqueda Miguel Rizzo Laura Alaniz Guillermo Mazzolini Slobodanka Klein Jean‐Michel Sallenave H. Eduardo Chuluyan 《Journal of cellular physiology》2013,228(2):469-475
Secretory leukocyte protease inhibitor (SLPI) is a serine protease inhibitor that was related to cancer development and metastasis dissemination on several types of tumors. However, it is not known the effect of SLPI on mammary and colon tumors. The aim of this study was to examine the effect of SLPI on mammary and colon tumor growth. The effect of SLPI was tested on in vitro cell apoptosis and in vivo tumor growth experiments. SLPI over‐expressing human and murine mammary and colon tumor cells were generated by gene transfection. The administration of murine mammary tumor cells over‐expressing high levels of SLPI did not develop tumors in mice. On the contrary, the administration of murine colon tumor cells over‐expressing SLPI, developed faster tumors than control cells. Intratumoral, but not intraperitoneal administration of SLPI, delayed the growth of tumors and increased the survival of mammary but not colon tumor bearing mice. In vitro culture of mammary tumor cell lines treated with SLPI, and SLPI producer clones were more prone to apoptosis than control cells, mainly under serum deprivation culture conditions. Herein we demonstrated that SLPI induces the apoptosis of mammary tumor cells in vitro and decreases the mammary but not colon tumor growth in vivo. Therefore, SLPI may be a new potential therapeutic tool for certain tumors, such as mammary tumors. J. Cell. Physiol. 228: 469–475, 2013. © 2012 Wiley Periodicals, Inc. 相似文献
100.
Catarina Prado e Castro José Paulo Sousa María Isabel Arnaldos João Gaspar María Dolores García 《法国昆虫学会纪事》2013,49(1-2):128-139
The first forensic entomological study performed in Portugal is presented. Two piglet (Sus scrofa L.) carcasses were used to determine adult Calliphoridae activity on carrion over a period of 121 days, all along the end of spring and the summer, both in a shaded and a sunny site. Five decomposition stages were observed and a total of 10723 adult Calliphoridae, belonging to 11 species, were collected. Calliphora vicina, Calliphora vomitoria, Chrysomya albiceps and Lucilia caesar were the dominant species in this study. Decomposition was faster on the carcass exposed to the sun and the number of Calliphoridae specimens was higher there than in the shaded site. It was found a significant effect of the decomposition stage in the number of specimens attracted to the carcass, as well as a significant effect of the interaction between the decomposition stage and insolation regime. Calliphora and Lucilia species did not show preference for sunny or shaded areas. Important differences in the Calliphoridae community structure were found compared to other regions of the Iberian Peninsula, reinforcing the need of further studies in different environments and regions of this geographical area in order to collect information about the local necrophagous fauna used in forensic practice. 相似文献