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151.
Estimating larval retention at individual reefs by local scale three-dimensional flows is a significant problem for understanding,
and predicting, larval dispersal. Determining larval dispersal commonly involves the use of computationally demanding and
expensively calibrated/validated hydrodynamic models that resolve reef wake eddies. This study models variation in larval
retention times for a range of reef shapes and circulation regimes, using a reef-scale three-dimensional hydrodynamic model.
It also explores how well larval retention time can be estimated based on the “Island Wake Parameter”, a measure of the degree
of flow turbulence in the wake of reefs that is a simple function of flow speed, reef dimension, and vertical diffusion. The
mean residence times found in the present study (0.48–5.64 days) indicate substantial potential for self-recruitment of species
whose larvae are passive, or weak swimmers, for the first several days after release. Results also reveal strong and significant
relationships between the Island Wake Parameter and mean residence time, explaining 81–92% of the variability in retention
among reefs across a range of unidirectional flow speeds and tidal regimes. These findings suggest that good estimates of
larval retention may be obtained from relatively coarse-scale characteristics of the flow, and basic features of reef geomorphology.
Such approximations may be a valuable tool for modeling connectivity and meta-population dynamics over large spatial scales,
where explicitly characterizing fine-scale flows around reef requires a prohibitive amount of computation and extensive model
calibration. 相似文献
152.
Nayelli Marsch‐Martínez Pieter B. F. Ouwerkerk Jeanneth Pablo‐Villa Paulina Lozano‐Sotomayor Raffaella Greco Patricia Ballester Vicente Balanzá Suzanne J. H. Kuijt Annemarie H. Meijer Andy Pereira Cristina Ferrándiz Stefan de Folter 《The Plant journal : for cell and molecular biology》2014,80(1):69-81
153.
154.
Paulina Akeus Veronica Langenes Astrid von Mentzer Ulf Yrlid Åsa Sjöling Pushpa Saksena Sukanya Raghavan Marianne Quiding-Järbrink 《Cancer immunology, immunotherapy : CII》2014,63(8):807-819
Tumor progression in the colon moves from aberrant crypt foci to adenomatous polyps to invasive carcinomas. The composition of the tumor-infiltrating leukocyte population affects the ability of the immune system to fight the tumor. T cell infiltration into colorectal adenocarcinomas, particularly T helper 1 (Th1) type T cells as well as increased regulatory T cell (Treg) frequencies, is correlated with improved prognosis. However, whether Th1 cells and Tregs are already present at the adenoma stage is not known. In this study, the APCMin/+ mouse model of intestinal adenomatous polyposis was used to investigate tumor-associated lymphocyte subsets and the mechanisms of their accumulation into gastrointestinal adenomas. Compared to unaffected tissue, adenomas accumulated CD4+FoxP3+ putative Treg in parallel with lower frequencies of conventional T cells and B cells. The accumulation of Treg was also observed in human adenomatous polyps. Despite high Treg numbers, the function of conventional T cells present in the APCMin/+ adenomas was not different from those in the unaffected tissue. Adenomas displayed an altered chemokine balance, with higher CCL17 and lower CXCL11 and CCL25 expression than in the unaffected tissue. In parallel, CXCR3+ Tregs were largely absent from adenomas. The data indicate that already in early stages of tumor development, the balance of lymphocyte-recruiting chemokines is altered possibly contributing to the observed shift toward higher frequencies of Treg. 相似文献
155.
Grzegorz Góralski Andrzej Judasz Paulina Gacek Aleksandra Grabowska-Joachimiak Andrzej J. Joachimiak 《Plant Systematics and Evolution》2014,300(2):225-238
Chromosome numbers, mainly for Polish flora, were examined in order to investigate whether such features as chromosome numbers and polyploid frequencies are correlated with a plant’s origin (native vs. alien) and invasiveness. Polyploid frequencies were estimated using three methods: the 11 and 14 thresholds and the 3.5 x value. Comparisons of the 2n values were done on different levels: in all angiosperms and in dicots and monocots separately. Invasive and non-invasive plants were compared in the entire dataset and in alien species only. Significant differences in both chromosome numbers and polyploid frequencies between alien and native species were observed. In most cases, native plants had more chromosomes and were more abundant in polyploids than in alien species. Also, monocots had higher polyploid frequencies than dicots. Comparisons of invasive and non-invasive plants done for all of the data and only for alien species showed that invasive species generally had more chromosomes and polyploids were more frequent in them than in the latter group; however, these differences were not always statistically significant. Possible explanations for these observations are discussed. 相似文献
156.
Tom A. R. Price Amanda Bretman Ana C. Gradilla Julia Reger Michelle L. Taylor Paulina Giraldo-Perez Amy Campbell Gregory D. D. Hurst Nina Wedell 《Proceedings. Biological sciences / The Royal Society》2014,281(1783)
The extent of female multiple mating (polyandry) can strongly impact on the intensity of sexual selection, sexual conflict, and the evolution of cooperation and sociality. More subtly, polyandry may protect populations against intragenomic conflicts that result from the invasion of deleterious selfish genetic elements (SGEs). SGEs commonly impair sperm production, and so are likely to be unsuccessful in sperm competition, potentially reducing their transmission in polyandrous populations. Here, we test this prediction in nature. We demonstrate a heritable latitudinal cline in the degree of polyandry in the fruitfly Drosophila pseudoobscura across the USA, with northern population females remating more frequently in both the field and the laboratory. High remating was associated with low frequency of a sex-ratio-distorting meiotic driver in natural populations. In the laboratory, polyandry directly controls the frequency of the driver by undermining its transmission. Hence we suggest that the cline in polyandry represents an important contributor to the cline in sex ratio in nature. Furthermore, as the meiotic driver causes sex ratio bias, variation in polyandry may ultimately determine population sex ratio across the USA, a dramatic impact of female mating decisions. As SGEs are ubiquitous it is likely that the reduction of intragenomic conflict by polyandry is widespread. 相似文献
157.
Jakub Karczmarski Tymon Rubel Agnieszka Paziewska Michal Mikula Mateusz Bujko Paulina Kober Michal Dadlez Jerzy Ostrowski 《Clinical proteomics》2014,11(1):24
Background
Histone post-translational modifications (PTMs) play an important role in the regulation of the expression of genes, including those involved in cancer development and progression. However, our knowledge of PTM patterns in human tumours is limited.Methods
MS-based analyses were used to quantify global alterations of histone PTMs in colorectal cancer (CRC) samples. Histones isolated from 12 CRCs and their corresponding normal mucosa by acidic extraction were separated by SDS-PAGE and analysed by liquid chromatography-mass spectrometry.Results
Among 96 modified peptides, 41 distinct PTM sites were identified, of which 7, 13, 11, and 10 were located within the H2A, H2B, H3, and H4 sequences, respectively, and distributed among the amino-terminal tails and the globular domain of the four histones. Modification intensities were quantified for 33 sites, of which 4 showed significant (p-value ≤ 0.05) differences between CRC tissues and healthy mucosa samples. We identified histone H3 lysine 27 acetylation (H3K27Ac) as a modification upregulated in CRC, which had not been shown previously.Conclusions
The present results indicate the usefulness of a bottom-up proteomic approach for the detection of histone modifications at a global scale. The differential abundance of H3K27Ac mark in CRC, a PTM associated with active enhancers, suggests its role in regulating genes whose expression changes in CRC. 相似文献158.
Maximiliano A. Sepúlveda Randall S. Singer Eduardo A. Silva-Rodríguez Antonieta Eguren Paulina Stowhas Katherine Pelican 《EcoHealth》2014,11(3):409-419
Infectious diseases, in particular canine distemper virus (CDV), are an important threat to the viability of wild carnivore populations. CDV is thought to be transmitted by direct contact between individuals; therefore, the study of species interactions plays a pivotal role in understanding CDV transmission dynamics. However, CDV often appears to move between populations that are ecologically isolated, possibly through bridge hosts that interact with both species. This study investigated how an introduced species could alter multihost interactions and act as a bridge host in a novel carnivore assemblage of domestic dogs (Canis familiaris), invasive American mink (Neovison vison), and threatened river otters (Lontra provocax) in southern Chile. We found that rural dogs interact with mink near farms whereas in riparian habitats, minks and river otters shared the same latrines with both species visiting sites frequently within time intervals well within CDV environmental persistence. No interactions were observed between dogs and otters at either location. Both dog and mink populations were serologically positive for CDV, making the pathogen transfer risk to otters a conservation concern. Altogether, introduced mink in this ecosystem have the potential to act as bridge hosts between domestic dogs and endangered carnivores. 相似文献
159.
Aneta Ksi??ek Karol Zub Paulina A. Szafrańska Monika Wieczorek Marek Konarzewski 《Biology letters》2014,10(12)
Despite its presumed significance, the association between immune defence, energy expenditures and overwinter survival is rarely studied. We analysed individual variation in immunocompetence quantified as neutrophil-to-lymphocyte ratio (N/L), total white blood cells (WBC) and natural antibody levels, along with resting (RMR) and peak metabolic rates (PMR) and mortality during three consecutive winter seasons in a natural population of the root vole, Microtus oeconomus. In early winter, WBC count was negatively correlated with RMR, whereas N/L ratio was negatively correlated with swim-elicited PMR. We suggest that while the first correlation reflected the trade-off between energy allocation in immunocompetence and other metabolically demanding processes, the latter correlation stemmed from stress-induced immunosuppression elicited by the necessity to cope with swimming in frequently flooded habitat. In addition, the analysis carried out during the first year of study characterized by a high population density and prevalence of infestation with a blood parasite—Babesia spp., showed that its intensity was inversely correlated with the N/L ratio. In summary, our results suggest that elevated N/L ratio increases the winter survival of free-ranging rodents by increasing their ability to cope with parasitic infections. 相似文献
160.
Iwona Rybakowska Ewa M. Slominska Paweł Romaszko Marcin Lipiński Paulina Żukowska Ryszard T. Smolenski 《Nucleosides, nucleotides & nucleic acids》2014,33(4-6):347-352
AMP-regulated protein kinase (AMPK) is involved in numerous regulatory processes and its role in control of cardiac energy metabolism is particularly important. This activity could be affected by AMP-deaminase (AMPD) since substrate of AMPD is AMPK activator. Hearts of male mouse, fed for six weeks with normal or high-fat diet, were fractionated to enrich AMPK activity. Purified fraction was incubated with AMARA peptide for up to 5 minutes and then conversion of AMARA to pAMARA was determined by liquid chromatography—mass spectrometry (LC/MS) using mass detector. Activity of AMPK in heart was 0.038 ± 0.012 pmol/min/mg protein for mice fed high-fat diet and that was not different to control (0.032 ± 0.01 pmol/min/mg protein). We observed change in AMPD activity. It was 5.39 ± 1.5 nmol/mg tissue/min in heart of mice fed high-fat diet while in heart of mice fed low-fat diet it was 2.29 ± 0.32 nmol/mg tissue/min. Data we present indicate that while total AMPK activity is not changed decrease in AMPD activity may affect AMPK signaling in diabetic heart. 相似文献