The action of an enzymatically stable Leu-enkephalin analog on the prostanoid content in the myocardium under stress- and adrenaline-induced damage

The protective action of enzymatically stable analogue of Leu-enkephalin (D-ala-2-leu5-arg6), injected intraperitoneally, in the course of stress- and epinephrine induced myocardial damage was demonstrated in animal (129 white rats) experiments. Two effect of enkephalin were sufficient for the cardiac protection: enkephalin-stimulated prostacyclin biosynthesis and simultaneous inhibition of thromboxane production.     

Erythrocyte antigen: isolation, biochemical and immunologic properties]

The authors suggest a simple method of obtaining erythrocytic antigen in considerable amounts. This antigen is of stromal origin, contains from 10 to 20% protein, and is relatively homogenous. With the concentration of from 1 to 50 microgram by protein the preparation represents a transparent solution; with greater concentrations the antigen is white, turbid, but is well dissolved and convenient for administration to the animals. In case of a single administration without any adjuvants the antigen is highly immunogenic in low doses by protein. To the optimal immunizing dose of erythrocytes (5 X 10(8)) correspond about 100 microgram of the antigen by protein. The primary response to the antigen is similar to the response to sheep red blood cells (SRBC). It is exceedingly effective for the formation of immunological memory. The level of secondary responses in the adoptive transfer to all the EAG doses always exceeded the secondary response to SRBC. By adding EAG into agar during the local hemolysis in gel test determined the avidity of the antibodies synthesized at various periods of the immune response to SRBC.