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951.
Collaborative experiments were conducted to determine whether microbial populations associated with plant roots may artifactually
affect the rates of Fe uptake and translocation from microbial siderophores and phytosiderophores. Results showed nonaxenic
maize to have 2 to 34-fold higher Fe-uptake rates than axenically grown plants when supplied with 1 μM Fe as either the microbial siderophore, ferrioxamine B (FOB), or the barley phytosiderophore, epi-hydroxymugineic acid (HMA).
In experiments with nonsterile plants, inoculation of maize or oat seedlings with soil microorganisms and amendment of the
hydroponic nutrient solutions with sucrose resulted in an 8-fold increase in FOB-mediated Fe-uptake rates by Fe-stressed maize
and a 150-fold increase in FOB iron uptake rates by Fe-stressed oat, but had no effect on iron uptake by Fe-sufficient plants.
Conversely, Fe-stressed maize and oat plants supplied with HMA showed decreased uptake and translocation in response to microbial
inoculation and sucrose amendment. The ability of root-associated microorganisms to affect Fe-uptake rates from siderophores
and phytosiderophores, even in short-term uptake experiments, indicates that microorganisms can be an unpredictable confounding
factor in experiments examining mechanisms for utilization of microbial siderophores or phytosiderophores under nonsterile
conditions. 相似文献
952.
Jean-Thomas Vilquin Philippe Kennel Paul Fonteneau Jean-Marie Warter Philippe Poindron 《Biotechnology letters》1992,14(10):875-880
Summary When grown in DMEM supplemented with 10 % fetal calf serum and using Cytodex 3® as microcarriers, TE671 cells entering the stationary phase optimally expressed acetylcholine receptors. These, receptors could be conveniently extracted from cell-saturated, microbeads or monodispersed cells obtained by trypsinization of microbeads. Typically, a 500 ml-batch gave 6–7 pmol of receptors which could be used as antigen to assay anti-acetylcholine receptor antibodies in the sera of myasthenic patients. 相似文献
953.
The relationship between ETS (electron transport system) activity and oxygen consumption in lake plankton: a cross-system calibration 总被引:4,自引:0,他引:4
The ETS (electron transport system) assay to measure respirationof aquatic organisms has been widely applied in studies of marinemetabolism, but its use in freshwaters has been much more limited.This method calculates oxygen consumption from the measuredETS activity using an empirical conversion factor. This factorhas been calculated for various marine organisms, and for naturalplankton communities, but calibrations for freshwater organismsare lacking. The aim of this paper was to determine the relationshipbetween lake plankton respiration and ETS activity, based onmeasured epilimnetic plankton oxygen uptake and ELS activityin 20 southern Quebéc lakes. The relationship betweenplankton oxygen consumption and ETS varies significantly bothwithin lakes over the growing season, and among lakes. The magnitudeof the error associated with calculating respiration from ETSis, however, similar to the error in other standard limnologicalprocedures used in plankton carbon flow studies. Oxygen consumptionis not a linear function of ETS across the range of lakes, butis rather a power function. The respiration:ETS ratio for lakeplankton therefore not constant: it is high in oligotrophicand colored lakes, and declines with trophy. These results areconsistent with the changes expected in the structure of theplankton along trophic gradients. 相似文献
954.
Elizabeth J. Cartwright Pauline Harrington Louise Norbury Gareth Leeming Paul T. Sharpe 《Bioscience reports》1992,12(1):57-67
Rat sperm isolated from the caput and caudal epididymis and the vas deferens were subjected to multiple partition in aqueous two-phase systems. The technique was used to reveal heterogeneity of a sperm population with respect to particular surface properties. Sperm from all three regions gave broad distributions indicative of heterogeneous cell populations. Greatest heterogeneity was observed for cauda sperm with caput and was sperm producing similar distributions.Following multiple partition sperm from different regions of the distribution profiles were immunostained with three antibodies known to recognise maturation antigens. The results show that some antigens are acquired during epididymal transit whilst others are present throughout.The partition (surface heterogeneity) seen cannot therefore be explained solely by the distribution of the antigens recognised by 2D6, 6B2 and 3D5. 相似文献
955.
Henrik Kibak Lincoln Taiz Thomas Starke Paul Bernasconi Johann Peter Gogarten 《Journal of bioenergetics and biomembranes》1992,24(4):415-424
Proton pumping ATPases/ATPsynthases are found in all groups of present-day organisms. The structure of V- and F-type ATPases/ATP synthases is very conserved throughout evolution. Sequence analysis shows that the V- and F-type ATPases evolved from the same enzyme already present in the last common ancestor of all known extant life forms. The catalytic and noncatalytic subunits found in the dissociable head groups of the V/F-type ATPases are paralogous subunits, i.e., these two types of subunits evolved from a common ancestral gene. The gene duplication giving rise to these two genes (i.e., encoding the catalytic and noncatalytic subunits) predates the time of the last common ancestor.Mapping of gene duplication events that occurred in the evolution of the proteolipid, the noncatalytic and the catalytic subunits, onto the tree of life leads to a prediction for the likely subunit structure of the encoded ATPases. A correlation between structure and function of V/F-ATPases has been established for present-day organisms. Implications resulting from this correlation for the bioenergetics operative in proto-eukaryotes and in the last common ancestor are presented. The similarities of the V/F-ATPase subunits to an ATPase-like protein that was implicated to play a role in flagellar assembly are evaluated.Different V-ATPase isoforms have been detected in some higher eukaryotes. These data are analyzed with respect to the possible function of the different isoforms (tissue specific, organelle specific) and with respect to the point in their evolution when these gene duplications giving rise to the isoforms had occurred, i.e., how far these isoforms are distributed. 相似文献
956.
Joan Sloan Tracy A. Warner Paul T. Scott Trudi L. Bannam David I. Berryman Julian I. Rood 《Plasmid》1992,27(3)
A newClostridium perfringens-Escherichia coli shuttle plasmid has been constructed and its complete DNA sequence compiled. The vector, pJIR418, contains the replication regions from theC. perfringens replicon pIP404 and theE. coli vector pUC18. The multiple cloning site and lacZ gene from pUC18 are also present, which means that X-gal screening can be used to select recombinants inE. coli. Both chloramphenicol and erythromycin resistance can be selected inC. perfringens andE. coli since pJIR418 carries theC. perfringens catP and ermBP genes. Insertional inactivation of either the catP or ermBP genes can also be used to directly screen recombinants in both organisms. The versatility of pJIR418 and its applicability for the cloning of toxin genes fromC. perfringens have been demonstrated by the manipulation of a cloned gene encoding the production of phospholipase C. 相似文献
957.
Pierre Valeix Paul Preziosi Claude Rossignol Marie-Alice Farnier Serge Hercberg 《Biological trace element research》1992,32(1-3):259-266
Urinary iodine excretion was assessed in 642 healthy children aged 10 mo (n=243), 2 yr (n=183), and 4 yr (n=216) living in the Paris area and originating from continental France (60.3%), North Africa (13.8%), the West Indies (9.1%),
West Africa (8.3%), Southeast Asia (4.8%), and southern Europe (3.8%). Mild impairment of neurological (reflexes, tone, audiometry)
and intellectual development (Brunet-Lézine scale) was assessed in relation to iodine status. Iodine excretions (median values)
were 18.4, 11.9, and 10.9 μg/100 mL at 10 mo, 2 yr, and 4 yr, respectively, and risk of mild iodine deficiency (5–10 μg/100
mL) was 18.1%, 34.8%, and 38.3% for the same age groups. No relationship was found between anthropometry, global development
quotient, and iodine status. High hearing thresholds were more commonly associated with lower iodine excretion, suggesting
mild hearing defects. In spite of iodine prophylaxis, the risk of mild to moderate iodine deficiency still exists in France
and in a number of European countries. Evaluation of neurological sequels of borderline iodine status is a major public health
problem in European communities. 相似文献
958.
Momčilo Mihailović Paul Lindberg Ivan Jovanović Duŝan Antić 《Biological trace element research》1992,33(1-3):71-77
The selenium (Se) contents in common cereals in endemic and nonendemic areas in Serbia are very low. Plasma Se levels of both
patients and healthy subjects, were also low, reflecting low Se intakes. Patients with Balkan endemic nephropathy (BEN) had
significantly lower (p<0.05) plasma Se levels than healthy individuals, both from regions close to endemic areas, and from Belgrade. Mean plasma
Se of BEN patients was slightly but insignificantly higher in samples taken immediately after dialysis than in those taken
before, suggesting that very little of the Se present in plasma is dialyzable. Plasma SeGSH-Px activities before and after
hemodialysis in both BEN and Nonendemic chronic renal failure (NCRF) patients were not significantly different, but BEN patients
had lower enzyme activities than those with NCRF and healthy controls. In BEN patients, a significant correlation between
plasma Se and SeGSH-Px activity was found.
NCFR patients were with diagnoses: TBC of kidneys, chronic glomerulonephritis, chronic pyelonephritis, and polycystic kidneys. 相似文献
959.
Three new species of coccidian were recovered from the intestinal contents and faeces of lizards in Namibia, southwest Africa. Oöcysts of Eimeria barnardi n. sp. are described from Rhoptropus barnardi (Gekkonidae) and are ellipsoidal, 24.3 × 19.9 (21–26.5 × 16–22) m; shape index (length/width) 1.22 (1.12–1.30). A micropyle and oöcyst residuum are absent but a fragmented polar granule is present. Sporocysts are subspherical, 9.2 × 8.3 (8–11 × 7.5–9) m; shape index 1.11 (1.02–1.27). Oöcysts of Eimeria pachybibroni n. sp. were found in Pachydactylus bibroni (Gekkonidae) and are ellipsoidal, 26.2 × 18.2 (21.5–28 × 16–19) m; shape index 1.44 (1.30–1.52). A micropyle and oöcyst residuum are absent but a polar granule is present. Sporocysts are subspherical, 8.9 × 8.0 (8–9.5 × 7–8.5) m; shape index 1.12 (1.03–1.20). Oöcysts of Isospora spilogaster n. sp. are reported from Mabuya spilogaster (Scincidae) and are subspherical, 27.4 × 26.0 (21.5–35 × 21–35) m; shape index 1.05 (1.00–1.13). Micropyle, oöcyst residuum and polar granules are absent. Sporocysts are ellipsoidal, 13.2 × 9.7 (10.5–15 × 9–11) m; shape index 1.36 (1.08–1.50). 相似文献
960.
Pilar Galan Helene Thibault Paul Preziosi Serge Hercberg 《Biological trace element research》1992,32(1-3):421-426
The relationship between iron status and capacity for IL-2 production by lymphocytes was assessed in 81 children from 6 mo
to 3 yr of age selected at random from a population with low socioeconomic status, undergoing free systematic examination
in four children's health centers in the Paris area. Iron deficiency was defined by the existence of at least two abnormal
values among the three indicators of iron status: serum ferritin level ≤12 μg/L, transferrin saturation <12%, and erythrocyte
protoporphyrin concentration >3 μg/g hemoglobin. According to this definition, 53 children were classified as iron deficient
and 28 as iron sufficient. No differences were observed between the iron-deficient and iron-sufficient groups in terms of
the IL-2 concentration without stimulation by PHA. IL-2 production by lymphocytes stimulated with PHA, as well as the stimulation
index (ratio of IL-2 concentration following stimulation by PHA to that of IL-2 concentration without stimulation by PHA)
were significantly lower in iron-deficient children. The reduction in IL-2 production by activated lymphocytes observed in
our study of iron-deficient children may be responsible for impairments in immunity found by other authors, particularly in
cell-mediated immunity. 相似文献