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31.
S D Power M A Lochrie T E Patterson R O Poyton 《The Journal of biological chemistry》1984,259(10):6571-6574
The amino acid sequence of subunit VIII from yeast cytochrome c oxidase is reported. This 47-residue (Mr = 5364) amphiphilic polypeptide has a polar NH2 terminus, a hydrophobic central section, and a dilysine COOH terminus. An analysis of local hydrophobicity and predicted secondary structure along the peptide chain predicts that the hydrophobic central region is likely to be transmembranous. Subunit VIII from yeast cytochrome c oxidase exhibits 40.4% homology to bovine heart cytochrome c oxidase subunit VIIc , at the level of primary structure. Secondary structures and hydrophobic domains predicted from the sequences of both polypeptides are also highly conserved. From the location of hydrophobic domains and the positions of charged amino acid residues we have formulated a topological model for subunit VIII in the inner mitochondrial membrane. 相似文献
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A mutant of E. coli, T
s68b, selected as unable to grow at 43 C, is unable to synthesize proteins at 43 C, though it can carry out this function at 30 C. This defect is shown to be in the protein-synthetic rather than the RNA-synthetic machinery by an analysis of the response of the strain to infection with the RNA bacteriophage f2. An analysis of the capacity for RNA synthesis and the polyribosome content of these cells at 44 C indicates that the defect resides in the elongation step of protein synthesis. No defects could be detected in vitro. The results are discussed in light of similar data on other mutants and in relation to the general approach of analyzing complex mutants selected with ill-defined phenotypes.This work was supported by Grant GM14368 from the National Institute of Health. 相似文献
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Sterols of Chlorella. II. The Occurrence of an Unusual Sterol Mixture in Chlorella vulgaris 总被引:2,自引:2,他引:0 下载免费PDF全文
Patterson GW 《Plant physiology》1967,42(10):1457-1459
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Glenn W. Patterson 《Journal of phycology》1967,3(1):22-23
Cultures of Chlorella vulgaris were grown aulo-trophically under fluorescent light and heterotrophically on glucose and inorganic salts. Hydrocarbons were extracted and analyzed by gas-liquid chromatography, molecular sieve separations, and silicic acid-AgNO3 chromatography. Chlorella vulgaris grown under both culture conditions contained a series of saturated n-paraffins ranging from 17 to 36 carbon atoms in length. This is in contrast to reports in the early literature which indicated that the hydrocarbon fraction of algae was composed of only 1 or 2 specific hydrocarbons. Only under heterotrophic conditions, however, did C. vulgaris produce 1-penta-cosene and 1-heptacosene as the primary components of the hydrocarbon mixture. Other Chlorella species were examined, but only C. vulgaris produced significant quantities of these compounds. 相似文献
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Genetic and physical mapping of a novel region close to the fragile X site on the human X chromosome 总被引:11,自引:0,他引:11
M. N. Patterson M. V. Bell J. Bloomfield T. Flint H. Dorkins S. N. Thibodeau D. Schaid G. Bren C. E. Schwartz b. Wieringa H. -H. Ropers D. F. Callen G. Sutherland U. Froster-Iskenius H. Vissing K. E. Davies 《Genomics》1989,4(4):570-578
We report the isolation and characterization of a novel DNA marker (1A1) in Xqter in the region of the fragile X. Genetic studies in families segregating for the fragile X syndrome suggest that 1A1 lies between the disease mutation and the distal locus, DXS52. Studies in normal and fragile X families show that 1A1 is tightly linked to DXS52 (Zmax = 17.20; theta max = 0.03) and F8 (Zmax = 7.01; theta max = 0.08). Multipoint mapping of families supports the order Xcen-DXS105-FRAXA-1A1-DXS52-(F8, DXS115)-Xqter. Pulsed-field gel electrophoresis (PFGE) studies demonstrate that 1A1 defines a new region of at least 2 Mb of DNA not physically linked to DXS52 or F8, thus extending the physical map of Xq27-qter to over 4 Mb. Complex partial digestion PFGE patterns, probably due to differing degrees of methylation, are observed with 1A1 in unrelated normal and fragile-X-positive individuals, whereas other distal markers give uniform digestion profiles. Physical data suggest that 1A1 lies in a region less CpG rich than other distal markers in Xq27-qter. 相似文献
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