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991.
Takashi Saitoh Jon Olav Vik Nils Chr. Stenseth Toshikazu Takanishi Shintaro Hayakashi Nobuo Ishida Masaaki Ohmori Toshio Morita Shigeru Uemura Masahiko Kadomatsu Jun Osawa Koji Maekawa 《Population Ecology》2008,50(2):159-167
We analysed the effects of Quercus crispula acorn abundance on the density dependence of the large Japanese wood mouse Apodemus speciosus using time series data (1992–2007). The data were obtained in a forest in northern Hokkaido, Japan, by live-trapping rodents
and directly counting acorns on the ground. Acorn abundance in one year clearly influenced the abundance of wood mice in the
following year in all models examined based on the Gompertz and Ricker model; in addition, the abundance of wood mice had
effects on the population. Acorn abundance influenced the strength of density dependence (intraspecific competition) of the
wood mouse population. When the abundance of acorns was high, density dependence was relaxed, and as a result the equilibrium
density at which the population growth rate decreased to zero became higher. Those effects of acorn abundance were regarded
as a nonlinear perturbation effect (sensu Royama 1992). The nonlinearity of density dependence was also detected; higher densities had stronger effects on population growth rates.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
992.
Background. The 7th Framework Programme for Research and Technological Development is helping the European Union to prepare for an integrative system for intelligent management of biodiversity knowledge. The infrastructure that is envisaged and that will be further developed within the Programme “Horizon 2020” aims to provide open and free access to taxonomic information to anyone with a requirement for biodiversity data, without the need for individual consent of other persons or institutions. Open and free access to information will foster the re-use and improve the quality of data, will accelerate research, and will promote new types of research. Progress towards the goal of free and open access to content is hampered by numerous technical, economic, sociological, legal, and other factors. The present article addresses barriers to the open exchange of biodiversity knowledge that arise from European laws, in particular European legislation on copyright and database protection rights.We present a legal point of view as to what will be needed to bring distributed information together and facilitate its re-use by data mining, integration into semantic knowledge systems, and similar techniques. We address exceptions and limitations of copyright or database protection within Europe, and we point to the importance of data use agreements. We illustrate how exceptions and limitations have been transformed into national legislations within some European states to create inconsistencies that impede access to biodiversity information.Conclusions. The legal situation within the EU is unsatisfactory because there are inconsistencies among states that hamper the deployment of an open biodiversity knowledge management system. Scientists within the EU who work with copyright protected works or with protected databases have to be aware of regulations that vary from country to country. This is a major stumbling block to international collaboration and is an impediment to the open exchange of biodiversity knowledge. Such differences should be removed by unifying exceptions and limitations for research purposes in a binding, Europe-wide regulation. 相似文献
993.
High temperature requirement protein A1 (HtrA1), a secreted serine protease of the HtrA family, is associated with a multitude of human diseases. However, the exact functions of HtrA1 in these diseases remain poorly understood. We seek to unravel the mechanisms of HtrA1 by elucidating its interactions with chemical or biological modulators. To this end, we screened a small molecule library of 500 bioactive compounds to identify those that alter the formation of extracellular HtrA1 complexes in the cell culture medium. An initial characterization of two novel hits from this screen showed that protoporphyrin IX (PPP-IX), a precursor in the heme biosynthetic pathway, and its metalloporphyrin (MPP) derivatives fostered the oligomerization of HtrA1 by binding to the protease domain. As a result of the interaction with MPPs, the proteolytic activity of HtrA1 against Fibulin-5, a specific HtrA1 substrate in age-related macular degeneration (AMD), was increased. This physical interaction could be abolished by the missense mutations of HtrA1 found in patients with cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL). Furthermore, knockdown of HtrA1 attenuated apoptosis induced by PPP-IX. These results suggest that PPP-IX, or its derivatives, and HtrA1 may function as co-factors whereby porphyrins enhance oligomerization and the protease activity of HtrA1, while active HtrA1 elevates the pro-apoptotic actions of porphyrin derivatives. Further analysis of this interplay may shed insights into the pathogenesis of diseases such as AMD, CARASIL and protoporphyria, as well as effective therapeutic development. 相似文献
994.
Absorption of medium-chain fatty acids by the dog colon 总被引:1,自引:0,他引:1
995.
996.
Christopher A. Mela Carrie Patterson William K. Thompson Francis Papay Yang Liu 《PloS one》2015,10(11)
We have developed novel stereoscopic wearable multimodal intraoperative imaging and display systems entitled Integrated Imaging Goggles for guiding surgeries. The prototype systems offer real time stereoscopic fluorescence imaging and color reflectance imaging capacity, along with in vivo handheld microscopy and ultrasound imaging. With the Integrated Imaging Goggle, both wide-field fluorescence imaging and in vivo microscopy are provided. The real time ultrasound images can also be presented in the goggle display. Furthermore, real time goggle-to-goggle stereoscopic video sharing is demonstrated, which can greatly facilitate telemedicine. In this paper, the prototype systems are described, characterized and tested in surgeries in biological tissues ex vivo. We have found that the system can detect fluorescent targets with as low as 60 nM indocyanine green and can resolve structures down to 0.25 mm with large FOV stereoscopic imaging. The system has successfully guided simulated cancer surgeries in chicken. The Integrated Imaging Goggle is novel in 4 aspects: it is (a) the first wearable stereoscopic wide-field intraoperative fluorescence imaging and display system, (b) the first wearable system offering both large FOV and microscopic imaging simultaneously, (c) the first wearable system that offers both ultrasound imaging and fluorescence imaging capacities, and (d) the first demonstration of goggle-to-goggle communication to share stereoscopic views for medical guidance. 相似文献
997.
Jon Lapham Jason P. Rife Peter B. Moore Donald M. Crothers 《Journal of biomolecular NMR》1997,10(3):255-262
Pulsed field-gradient NMR experiments can be used to measure thediffusion constants of nucleic acids. The diffusion constants measured inthis way for double-helical DNAs of defined length agree well both withtheory and with measurements done using other techniques. When applied toRNAs, this experi- ment easily distinguishes duplex RNAs from RNA hairpinsand thus it can solve one of the perennial problems faced by RNAspectroscopists, i.e. assessing whether their samples are monomeric or not. 相似文献
998.
Annual recruitment of Acanthaster planci over a 13 year period in a known juvenile refuge substratum on Suva Reef was very patchy in time and space. Macroscale recruitment events resulted in outbreaks over many thousands of hectares over 100 km of coastline whereas localised but intensive mesoscale events resulted in concenrated aggregations over areas of several tens of hectares. A very low level of recruitment occurred in most years but three intensive, macroscale recruitment events occurred in 1977, 1984 and 1987, and two localised but intensive recruitment events occurred in 1982 and 1983. Annual recruitment of A. planci between 1975–89 had no overt relationship with rainfall events, as proposed in the terrestrial run-off hypothesis on the cause of primary out-breaks. Growth rates within monitored cohorts were highly variable, but mean growth rates of two cohorts 1984 and 1987 were relatively similar. Mortality rates of three monitored cohorts were variable. Longevity of the 1977 cohort was 7–8 years, while that of the 1984 cohort was 2–3 years. Mortality of the 1987 cohort has been low to date (i.e. 4 years of age). Disease, attributed to an undescribed sporozoan pathogen, was responsible for two mass mortalities in the 1984 cohort, in 1984 and in 1986, and eventually resulted in the extinction of this cohort. Anecdotal reports indicate that outbreaks of A. planci are increasing in frequency and severity. According to Fijian reef fishermen, the starfish were low to moderate in abundance in the Suva area from the 1920s to the 1960s. A progressive build up occurred on some reefs in 1963–67 leading to a major outbreak episode from 1967–1971. The outbreaks were in a chronic phase between 1977–1989. Our findings demonstrate that there is no invariable pattern or process involved in the population dynamics of A. planci and that a single explanation of the A. planci phenomenon is therefore unlikely. 相似文献
999.
Barbara Class Jon E. Brommer 《Evolution; international journal of organic evolution》2020,74(7):1540-1550
Accurately estimating genetic variance components is important for studying evolution in the wild. Empirical work on domesticated and wild outbred populations suggests that dominance genetic variance represents a substantial part of genetic variance, and theoretical work predicts that ignoring dominance can inflate estimates of additive genetic variance. Whether this issue is pervasive in natural systems is unknown, because we lack estimates of dominance variance in wild populations obtained in situ. Here, we estimate dominance and additive genetic variance, maternal variance, and other sources of nongenetic variance in eight traits measured in over 9000 wild nestlings linked through a genetically resolved pedigree. We find that dominance variance, when estimable, does not statistically differ from zero and represents a modest amount (2-36%) of genetic variance. Simulations show that (1) inferences of all variance components for an average trait are unbiased; (2) the power to detect dominance variance is low; (3) ignoring dominance can mildly inflate additive genetic variance and heritability estimates but such inflation becomes substantial when maternal effects are also ignored. These findings hence suggest that dominance is a small source of phenotypic variance in the wild and highlight the importance of proper model construction for accurately estimating evolutionary potential. 相似文献
1000.
Arhamatoulaye Ma?ga Jon Merlin Elodie Marcon Céline Rouget Maud Larregola Bernard Gilquin Carole Fruchart-Gaillard Evelyne Lajeunesse Charles Marchetti Alain Lorphelin Laurent Bellanger Roger J. Summers Dana S. Hutchinson Bronwyn A. Evans Denis Servent Nicolas Gilles 《PloS one》2013,8(7)
ρ-Da1a is a three-finger fold toxin from green mamba venom that is highly selective for the α1A-adrenoceptor. This toxin has atypical pharmacological properties, including incomplete inhibition of 3H-prazosin or 125I-HEAT binding and insurmountable antagonist action. We aimed to clarify its mode of action at the α1A-adrenoceptor. The affinity (pKi 9.26) and selectivity of ρ-Da1a for the α1A-adrenoceptor were confirmed by comparing binding to human adrenoceptors expressed in eukaryotic cells. Equilibrium and kinetic binding experiments were used to demonstrate that ρ-Da1a, prazosin and HEAT compete at the α1A-adrenoceptor. ρ-Da1a did not affect the dissociation kinetics of 3H-prazosin or 125I-HEAT, and the IC50 of ρ-Da1a, determined by competition experiments, increased linearly with the concentration of radioligands used, while the residual binding by ρ-Da1a remained stable. The effect of ρ-Da1a on agonist-stimulated Ca2+ release was insurmountable in the presence of phenethylamine- or imidazoline-type agonists. Ten mutations in the orthosteric binding pocket of the α1A-adrenoceptor were evaluated for alterations in ρ-Da1a affinity. The D1063.32A and the S1885.42A/S1925.46A receptor mutations reduced toxin affinity moderately (6 and 7.6 times, respectively), while the F862.64A, F2886.51A and F3127.39A mutations diminished it dramatically by 18- to 93-fold. In addition, residue F862.64 was identified as a key interaction point for 125I-HEAT, as the variant F862.64A induced a 23-fold reduction in HEAT affinity. Unlike the M1 muscarinic acetylcholine receptor toxin MT7, ρ-Da1a interacts with the human α1A-adrenoceptor orthosteric pocket and shares receptor interaction points with antagonist (F862.64, F2886.51 and F3127.39) and agonist (F2886.51 and F3127.39) ligands. Its selectivity for the α1A-adrenoceptor may result, at least partly, from its interaction with the residue F862.64, which appears to be important also for HEAT binding. 相似文献