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Expression of C gamma 4 T cell receptors and lack of isotype exclusion by dendritic epidermal T cell lines 总被引:6,自引:0,他引:6
F Koning W M Yokoyama W L Maloy G Stingl T J McConnell D I Cohen E M Shevach J E Coligan 《Journal of immunology (Baltimore, Md. : 1950)》1988,141(6):2057-2062
Although four murine C gamma gene segments (C gamma 1, 2, 3, and 4) are known to exist, the large majority of expressed gamma-chains have been shown to be of the C gamma 1 isotype and no evidence exists for the expression of more than one receptor by gamma delta TCR-bearing cells. We investigated the nature of the TCR expressed on a number of murine dendritic epidermal T cell-derived cell lines by using both Northern blot and immunoprecipitation analyses. One of these CD3+ cell lines (T195) expresses C gamma 4, V gamma 1, and delta mRNA, and its CD3-associated TCR complex can be precipitated by both anti-C gamma 4 and anti-delta sera, indicating that this receptor is a C gamma 4/delta heterodimer. Furthermore, we show that two cell lines (Y245, Y93) express two distinct TCR gamma-chains, one derived from the C gamma 4 locus, whereas the second gamma-chain is probably derived from the C gamma 2 locus. Together with the previous demonstration of C gamma 1/delta TCR on a number of dendritic epidermal T cell lines (DETC), these results indicate that such DETC are capable of expressing a variety of gamma delta TCR and that, in some DETC, isotype exclusion of gamma-chain expression does not occur. 相似文献
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Development of tool use in a macaque and a gorilla 总被引:3,自引:0,他引:3
Francesco Natale Patrizia Poti' Giovanna Spinozzi 《Primates; journal of primatology》1988,29(3):413-416
The development of the capacity to use a stick as a tool was tested in a macaque (Macaca fuscata) and a gorilla (Gorilla gorilla gorilla) infants that had previously shown to be able to use strings and supports as dragging tools. Subjects were tested between
15 and 38 months of age. Different levels of competence between the subjects emerged over testing. The macaque developed a
stereotyped strategy to cope with the problem, only getting random successes, whilst the gorilla developed a flexible strategy
and revealed to be able to mentally represent the solution of the problem. In fact, when not successful using the stick, the
gorilla thought out an alternative strategy, choosing and adapting a new object to use it as a tool. 相似文献
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Bruna Tedeschi Patrizia Vernole M.Lucia Sanna Benedetto Nicoletti 《Human genetics》1992,89(5):543-547
Summary Chromosome fragile sites are inducible by aphidicolin in cultured human lymphocytes. To assess the frequency and distribution of these common fragile sites in the general population, a cytogenetic survey was performed on 126 subjects, 59 males and 67 females, whose age ranged from 1 day to 72 years. Common fragile sites, induced by aphidicolin, were widespread and showed a remarkably different sensitivity among individuals; age influenced the overall frequency of fragile sites. Moreover, both age and sex seemed to modulate the expression of specific fragile sites. In our population, the most common fragile sites were: 3p14, 16q23, Xp22, 6q26, 1p31, 4q31, 1p22, 7q22, 2q33, 3q27, 2q31, 7q32, 14q24, 10q22, 5q31, 2q37, 6p21. 相似文献
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