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961.
Phylogenetic analysis of 277 human G-protein-coupled receptors as a tool for the prediction of orphan receptor ligands 总被引:1,自引:0,他引:1
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Background
G-protein-coupled receptors (GPCRs) are the largest and most diverse family of transmembrane receptors. They respond to a wide range of stimuli, including small peptides, lipid analogs, amino-acid derivatives, and sensory stimuli such as light, taste and odor, and transmit signals to the interior of the cell through interaction with heterotrimeric G proteins. A large number of putative GPCRs have no identified natural ligand. We hypothesized that a more complete knowledge of the phylogenetic relationship of these orphan receptors to receptors with known ligands could facilitate ligand identification, as related receptors often have ligands with similar structural features.Results
A database search excluding olfactory and gustatory receptors was used to compile a list of accession numbers and synonyms of 81 orphan and 196 human GPCRs with known ligands. Of these, 241 sequences belonging to the rhodopsin receptor-like family A were aligned and a tentative phylogenetic tree constructed by neighbor joining. This tree and local alignment tools were used to define 19 subgroups of family A small enough for more accurate maximum-likelihood analyses. The secretin receptor-like family B and metabotropic glutamate receptor-like family C were directly subjected to these methods.Conclusions
Our trees show the overall relationship of 277 GPCRs with emphasis on orphan receptors. Support values are given for each branch. This approach may prove valuable for identification of the natural ligands of orphan receptors as their relation to receptors with known ligands becomes more evident. 相似文献962.
Gravitropism allows plant organs to guide their growth in relation to the gravity vector. For most roots, this response to gravity allows downward growth into soil where water and nutrients are available for plant growth and development. The primary site for gravity sensing in roots includes the root cap and appears to involve the sedimentation of amyloplasts within the columella cells. This process triggers a signal transduction pathway that promotes both an acidification of the wall around the columella cells, an alkalinization of the columella cytoplasm, and the development of a lateral polarity across the root cap that allows for the establishment of a lateral auxin gradient. This gradient is then transmitted to the elongation zones where it triggers a differential cellular elongation on opposite flanks of the central elongation zone, responsible for part of the gravitropic curvature. Recent findings also suggest the involvement of a secondary site/mechanism of gravity sensing for gravitropism in roots, and the possibility that the early phases of graviresponse, which involve differential elongation on opposite flanks of the distal elongation zone, might be independent of this auxin gradient. This review discusses our current understanding of the molecular and physiological mechanisms underlying these various phases of the gravitropic response in roots. 相似文献
963.
Regulation of alternative oxidase gene expression in soybean 总被引:13,自引:0,他引:13
Djajanegara I Finnegan PM Mathieu C McCabe T Whelan J Day DA 《Plant molecular biology》2002,50(4-5):735-742
Soybean (Glycine max cv. Stevens) suspension cells were used to investigate the expression of the alternative oxidase (Aox) multigene family. Suspension cells displayed very high rates of cyanide-insensitive respiration, but Aox3 was the only isoform detected in untreated cells. Incubation with antimycin A, citrate, salicylic acid or at low temperature (10 °C) specifically induced the accumulation of the Aox1 isoform. Aox2 was not observed under any conditions in the cells. Increases in Aox1 protein correlated with increases in Aox1 mRNA. Treatment of soybean cotyledons with norflurazon also induced expression of Aox1. Reactive oxygen species (ROS) were detected upon incubation of cells with antimycin, salicylic acid or at low temperature, but not during incubation with citrate. Aox1 induction by citrate, but not by antimycin, was prevented by including the protein kinase inhibitor staurosporine in the medium. The results suggest that multiple pathways exist in soybean to regulate expression of Aox genes and that Aox1 specifically is induced by a variety of stress and metabolic conditions via at least two independent signal transduction pathways. 相似文献
964.
von Aderkas Patrick Rohr René Sundberg Björn Gutmann Markus Dumont-BéBoux Nicole Lelu Marie-Anne 《Plant Cell, Tissue and Organ Culture》2002,69(2):111-120
Somatic embryos of Larix × leptoeuropaea were grown on modified MSG media with 60 M abscisic acid (ABA). These were compared to control embryos raised on the same medium without ABA. Transmission electron microscopy demonstrated that zonation of polyphenol production as well as presence of extracellular mucilage was markedly different in embryos raised with and without ABA. Idioblasts were found in subepidermal and pith regions of hypocotyls and among the subepidermal cells of cotyledons in embryos matured on ABA, but not in embryos matured without ABA. The embryonal root caps of ABA-treated embryos had substantial deposition of lipids and proteins in both the column and inner pericolumn regions, but not in the outer layer of the pericolumn. Control embryos showed no accumulation of proteins or lipids, but an increase in polyphenol accumulation, which had spread to the epidermal and sub-epidermal layers of the cotyledons and hypocotyl. Starch accumulation was similar over the course of development in embryos treated with or without ABA. Using gas chromatography-selected reaction monitoring mass spectrometry, it was shown that concentrations of ABA averaged 186 ± 17 g g–1 dry weight (DW) in embryos raised on medium supplemented with this plant growth regulator, versus an average concentration of 55 ± 19 g g–1 DW in embryos raised in the absence of ABA. No difference in ABA concentration was found between the root cap and the rest of ABA-treated embryos. 相似文献
965.
Endogenous levels of free and conjugated forms of three classes of planthormones were quantified at various stages of megagametophyte development inDouglas fir. Megagametophytes were excised weekly from 8–16 weeks pastpollination (WPP), a period encompassing the central cell to the earlymaturation stage of seed development. The hormones indole-3 acetic acid (IAA),indole-3-aspartate (IAAsp), zeatin (Z), zeatin riboside (ZR), isopentenyladenine(iP), isopentenyladenosine (iPA), abscisic acid (ABA) and abscisic acid glucoseester (ABA-GE) were extracted, purified, fractionated by high- performanceliquid chromatography (HPLC), and then quantified using an enzyme-linkedimmunosorbent assay (ELISA) method. Z levels ranged from 0–25ng/g dry weight (DW) and were highest in megagametophytes at thecentral cell stage (8 WPP). During embryogenesis, Z levels peakedduring week 13. In contrast, the ZR conjugate was not detected over the periodstudied. The iP content of megagametophytes increased at 10 and 13WPP, while the iPA concentration increased at 13 WPP.Prior to fertilisation, IAA was highest in megagametophytes at 9WPP. During embryogenesis, the major IAA accumulations occurred at11, 13 and 15 WPP, the concentration ranging from 0–0.43g/g DW. IAAsp concentrations reached their highest level duringembryogenesis at 14 WPP. ABA content increased at 11 and 13WPP, with a concentration range of 0.1–13 g/gDW. In contrast, ABA-GE levels were relatively constant over the 9-weekperiod analyzed. The endogenous levels of plant hormones varied withmegagametophyte development and were associated with morphological changes. 相似文献
966.
Stefano GB Zhu W Cadet P Mantione K Bilfinger TV Bianchi E Guarna M 《Neuro endocrinology letters》2002,23(1):21-26
The distribution of morphine-containing cells in the central nervous system, adrenal gland, and its presence in blood may serve to demonstrate that this signal molecule can act as a hormone besides its role in cell-to-cell signaling within the brain. This speculative review is the result of a literature evaluation with an emphasis on studies from our laboratory. Opioid peptides and opiate alkaloids have been found to influence cardiac and vascular function. They have also been reported to promote ischemic preconditioning protection in the heart. Given the presence of morphine and the novel mu(3) opiate receptor on vascular endothelial cells, including cardiac and vascular endothelial cells in the median eminence, it would appear that endogenous opiate alkaloids are involved in modulating cardiac function, possible at the hormonal level. This peripheral target tissue, via nitric oxide coupling to mu opiate receptors, may serve to down regulate the excitability of this tissue given the heart's high performance state as compared to that of the saphenous vein, a passive resistance conduit. With this in mind, morphine and other endogenous opiate alkaloids may function as a hormone. 相似文献
967.
Arredouani A Henquin JC Gilon P 《American journal of physiology. Endocrinology and metabolism》2002,282(5):E982-E991
Thapsigargin (TG), a blocker of Ca(2+) uptake by the endoplasmic reticulum (ER), was used to evaluate the contribution of the organelle to the oscillations of cytosolic Ca(2+) concentration ([Ca(2+)](c)) induced by repetitive Ca(2+) influx in mouse pancreatic beta-cells. Because TG depolarized the plasma membrane in the presence of glucose alone, extracellular K(+) was alternated between 10 and 30 mM in the presence of diazoxide to impose membrane potential (MP) oscillations. In control islets, pulses of K(+), mimicking regular MP oscillations elicited by 10 mM glucose, induced [Ca(2+)](c) oscillations whose nadir remained higher than basal [Ca(2+)](c). Increasing the depolarization phase of the pulses while keeping their frequency constant (to mimic the effects of a further rise of the glucose concentration on MP) caused an upward shift of the nadir of [Ca(2+)](c) oscillations that was reproduced by raising extracellular Ca(2+) (to increase Ca(2+) influx) without changing the pulse protocol. In TG-pretreated islets, the imposed [Ca(2+)](c) oscillations were of much larger amplitude than in control islets and occurred on basal levels. During intermittent trains of depolarizations, control islets displayed mixed [Ca(2+)](c) oscillations characterized by a summation of fast oscillations on top of slow ones, whereas no progressive summation of the fast oscillations was observed in TG-pretreated islets. In conclusion, the buffering capacity of the ER in pancreatic beta-cells limits the amplitude of [Ca(2+)](c) oscillations and may explain how the nadir between oscillations remains above baseline during regular oscillations or gradually increases during mixed [Ca(2+)](c) oscillations, two types of response observed during glucose stimulation. 相似文献
968.
Swanson PC 《Molecular and cellular biology》2002,22(5):1340-1351
Two lymphoid cell-specific proteins, RAG-1 and RAG-2, initiate V(D)J recombination by introducing DNA breaks at recombination signal sequences (RSSs). Although the RAG proteins themselves bind and cleave DNA substrates containing either a 12-RSS or a 23-RSS, DNA-bending proteins HMG-1 and HMG-2 are known to promote these processes, particularly with 23-RSS substrates. Using in-gel cleavage assays and DNA footprinting techniques, I analyzed the catalytic activity and protein-DNA contacts in discrete 12-RSS and 23-RSS complexes containing the RAG proteins and either HMG-1 or HMG-2. I found that both the cleavage activity and the pattern of protein-DNA contacts in RAG-HMG complexes assembled on 12-RSS substrates closely resembled those obtained from analogous 12-RSS complexes lacking HMG protein. In contrast, 23-RSS complexes containing both RAG proteins and either HMG-1 or HMG-2 exhibited enhanced cleavage activity and displayed an altered distribution of cleavage products compared to 23-RSS complexes containing only RAG-1 and RAG-2. Moreover, HMG-dependent heptamer contacts in 23-RSS complexes were observed. The protein-DNA contacts in RAG-RSS-HMG complexes assembled on 12-RSS or 23-RSS substrates were strikingly similar at comparable positions, suggesting that the RAG proteins mediate HMG-dependent heptamer contacts in 23-RSS complexes. Results of ethylation interference experiments suggest that the HMG protein is positioned 5' of the nonamer in 23-RSS complexes, interacting largely with the side of the duplex opposite the one contacting the RAG proteins. Thus, HMG protein plays the dual role of bringing critical elements of the 23-RSS heptamer into the same phase as the 12-RSS to promote RAG binding and assisting in the catalysis of 23-RSS cleavage. 相似文献
969.
Fertilities varying in time in an unpredictable manner raise the question of the maintenance of polymorphism, and the subsequent
question of the minimal total number of generations spent by the system in impoverished polymorphism. In the one-locus two-allele
model, level surfaces of this minimal time out of a given set K of constraints defining polymorphism are delineated. The surface of null minimal time is also the largest set of genotype
frequencies from which there exists at least one route remaining in K forever. The influence of the range of admissible fertilities clarifies the trade-off between homozygotes and heterozygotes.
Notably, ranges of fertility forbidding a system leaving K to ever return to it are determined. Generally, keeping a chance to regain polymorphism demands a sufficiently relatively
high fertility for matings involving heterozygotes.
Received: 5 January 2001 / Revised version: 20 October 2001 / Published online: 17 May 2002 相似文献
970.
Engineered viruses to select genes encoding secreted and membrane-bound proteins in mammalian cells 总被引:3,自引:1,他引:2
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Moffatt P Salois P Gaumond MH St-Amant N Godin E Lanctôt C 《Nucleic acids research》2002,30(19):4285-4294
We have developed a functional genomics tool to identify the subset of cDNAs encoding secreted and membrane-bound proteins within a library (the ‘secretome’). A Sindbis virus replicon was engineered such that the envelope protein precursor no longer enters the secretory pathway. cDNA fragments were fused to the mutant precursor and expression screened for their ability to restore membrane localization of envelope proteins. In this way, recombinant replicons were released within infectious viral particles only if the cDNA fragment they contain encodes a secretory signal. By using engineered viral replicons to selectively export cDNAs of interest in the culture medium, the methodology reported here efficiently filters genetic information in mammalian cells without the need to select individual clones. This adaptation of the ‘signal trap’ strategy is highly sensitive (1/200 000) and efficient. Indeed, of the 2546 inserts that were retrieved after screening various libraries, more than 97% contained a putative signal peptide. These 2473 clones encoded 419 unique cDNAs, of which 77% were previously annotated. Of the 94 cDNAs encoding proteins of unknown function, 24% either had no match in databases or contained a secretory signal that could not be predicted from electronic data. 相似文献