Eine Methode zur histochemischen Bestimmung von RNS und DNS an der gleichen Zelle

Zusammenfassung Es wird eine Methode beschrieben, die die quantitative cytophotometrische Bestimmung von DNS und RNS nebeneinander am gleichen Objekt gestattet. Dazu werden die Zellen mit Gallocyaninchromalaun und anschließend mit der Feulgen-Reaktion gefärbt, wobei ein Peulgenfarbstoff verwendet wird, der im blauen Spektralbereich absorbiert. Auch unter den veränderten Färbebedingungen verlaufen beide Parbreaktionen quantitativ. Es werden für die Durchführung der Methode die geeigneten Versuchsansätze und ein Verfahren zur Auswertung der cytophotometrischen Meßergebnisse angegeben.

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Microphotometric determination of DNA and RNA within the same cell

Summary A method is described which allows the simultanous cytophotometric determination of DNA and RNA within the same cell. Cells are stained with gallocyanine chromealum and then, in an additional step, by a Feulgen procedure in which Coriphosphin 0 was used as a Schiff type reagent to accomplish absorption in the blue region of the spectrum. It could be shown that under the altered conditions both staining reactions can be used for quantitative purposes. Methods for carrying out the preparation of the material, for cytophotometric measurements and for calculating RNA and DNA values are suggested.

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Mit Unterstützung durch die Deutsche Forschungsgemeinschaft.

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Wir widmen diese Arbeit dem Andenken Wolfgang Zellers. Wesentliche Teile dieser Arbeit waren als Teil seiner Dissertation vorgesehen. Sein tragischer Tod hat seine Promotion verhindert.     

THE DEGENERATIVE CHANGES IN PANCREATIC ACINAR CELLS CAUSED BY DL-ETHIONINE

Degeneration of pancreatic acinar cells in rats injected with ethionine was studied by electron microscopy. The most conspicuous morphologic lesions occurred in the ergastoplasm. There was a widening of the endoplasmic reticulum, a decrease in number of membrane-associated ribosomes, and a development of fine and coarse vacuoles containing agranular disoriented membranes. Cytoplasmic ribosomes unassociated with membranes were less numerous. Nuclear changes consisted of a coarsening and clumping of the nuclear chromatin, chromatin margination, and increased osmiophilia and vacuolation of the nucleolus. Eight to ten days after the beginning of ethionine injections, changes in zymogen granules, mitochondria, and the Golgi apparatus appeared, but only after extensive damage to the acinar cell. The effects were consistent with ethionine's known interference with protein metabolism but also suggest disturbance in ribonucleic acid metabolism. The ergastoplasmic changes after ethionine were similar in some respects to the early lesions produced in liver parenchymal cells by fasting, to the changes occurring in animals on protein-free diets, or to some of the liver changes produced by azo dye carcinogens. The ribosomal and ergastoplasmic changes represent early morphologic expressions of the biochemical effect of ethionine.     

Quantitative histochemische Untersuchungen über die säurefeste Anfärbung des Spermienkopfes

Zusammenfassung Die bei der sog. säurefesten Färbung im Spermienkopf enthaltene Kristallviolettmenge kann quantitativ mit cytophotometrischen Methoden bestimmt werden. Eine Fixierung mit abs. Alkohol und Färbung bei pH 7,5 ergab eine für quantitative Zwecke optimale Anfärbung. Die gebundene Kristallviolettmenge ist der DNS-Menge des Spermiums proportional. Es wird vermutet, daß die säurefeste Färbung auf einer Bindung des Kristallvioletts an die Phosphatgruppen der Nukleinsäuren beruht und daß die spezielle Anordnung der Nukleoproteine im Spermium eine wesentliche Rolle spielt.Mit Unterstützung der Deutschen Forschungs- Gemeinschaft.Stipendiat der Alexander v. Humboldt-Stiftung, 1962.     

A comparison of sperm morphology and silver nitrate staining characteristics in the domestic ferret and the black-footed ferret

Ejaculated sperm from the domestic ferret (Mustela putorius furo) and the black-footed ferret (Mustela nigripes) were compared for differences in morphological abnormalities and argentophilic protein distribution. Thawed domestic ferret sperm was also compared to fresh sperm to determine whether there were any effects on cell morphology due to cryopreservation. There were statistically significant differences between the two species of ferret in two of the categories scored. The domestic ferret had a higher frequency of cells that were bent in the midpiece and in the principal piece, and a higher frequency of headless and tailless cells when compared to the black-footed ferret. There were no statistically significant differences in cell morphology between the fresh and cryopreserved ejaculates of the domestic ferret employing a standard egg yolk cryoextender. Silver nitrate staining distribution was different between the two species in both the head and tail region.