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61.
Abstract: A chronic hyperphenylalanemia was effectively produced in developing mice by daily administrations of phenylalanine (2 mg/g body wt) and a phenylalanine hydroxylase inhibitor α-methyl-D, L-phenylalanine (0.43 mg/g body wt). The presence of α-methylphenylalanine in newborn mice inhibited 65–70% of hepatic phenylalanine hydroxylase activity within 12 h. Since this maximum inhibition persisted for 24 h or longer, decreased enzyme activity was maintained by daily administrations. Whereas concentrations of phenylalanine increased approximately 40-fold in both plasma and brain following injection of α-methylphenylalanine and phenylalanine, plasma levels of tyrosine were not altered significantly. Concomitant with changes in phenylalanine concentrations we observed the brain polyribosomes' disaggregation, which reached a maximum 3 h after injection and persisted as long as 18 h. Polyribosomes did not become refractory to as many as 10 daily injections of α-methylphenylalanine and phenylalanine. In addition to polyribosome disaggregation, chronic hyperphenylalanemia reduced the rates of polypeptide chain elongation on polyribosomes isolated from brain homogenates. 相似文献
62.
Vidmantas A. Raisys Patrick N. Friel Patricia R. Graaff Kent E. Opheim Alan J. Wilensky 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》1980,183(4)
A one-step method for extraction of diazepam, nordiazepam, and internal standard into toluene is followed by chromatographic separation and detection with either dual-wavelength high-performance liquid chromatography or electron-capture gas—liquid chromatography. Agreement between the two methods was excellent for diazepam (r = 0.99, n = 38) and good for nordiazepam (r = 0.96, n = 79) over a concentration range that included subtherapeutic, therapeutic, and toxic plasma levels. 相似文献
63.
Caulanthus barnebyi from northern Nevada is described, illustrated, and compared with its nearest relative,C. glaucus S. Wats. 相似文献
64.
Asclepias welshii, restricted to the Coral Pink Sand Dunes of southern Utah, is described and illustrated. Its relationship to other species of seriesRoseae is discussed. 相似文献
65.
66.
Jeffrey B. Krahling Robert Gee Patricia A. Murphy James R. Kirk N.E. Tolbert 《Biochemical and biophysical research communications》1978,82(1):136-141
Oxygen uptake with succinate or palmitoyl-CoA as substrates can be measured in rat liver mitochondria that have been isolated by sucrose density gradient centrifugation providing the fractions are diluted with a 30 mM phosphate buffer rather than with an isotonic medium. Separate assay procedures were used to measure peroxisomal and mitochondrial β-oxidation of palmitoyl-CoA in the fractions of a sucrose gradient used to separate these organelles. A preliminary estimate of the ratio of palmitoyl-CoA oxidation by the mitochondrial fraction relative to the surviving peroxisomes from livers of male rats was 3.2. 相似文献
67.
The distribution pattern of silver-NORs was studied in cells of six-day blastocysts and kidney fibroblasts of the rabbit using
the Ag-AS technique. At metaphase and interphase there was a binomial distribution of the number of stained sites in both
populations but blastocysts had a greater percentage of cells with larger numbers of stained sites. Up to 7 of the 8 chromosomes
known to bear NORs were stained in cells from blastocysts while a maximum of 6 were stained in fibroblasts. A significant
difference was found between the mean numbers of chromosomal NORs per cell in metaphases from blastocysts and fibroblasts,
where they were 4.2 and 3.3 respectively. Similarly, the mean number of NORs in interphase was significantly greater in cells
from blastocysts. The distribution of staining on chromosome pair 13 was related to cell type. Significantly more cells in
blastocysts than fibroblasts showed staining in this chromosome pair. 相似文献
68.
The red marine algae Microcladia borealis, M. californica and M. coulteri produce several unusual halogenated monoterpenes including violacene, plocamene-B, plocamene-C, and plocamane-D. The isolation of these terpenes along with a study of their variation in each Microcladia at different locations are described. 相似文献
69.
Mouse testis cells have been separated by equilibrium density centrifugation in gradients of Renografin. Intact testis cells were not damaged by the separation procedure provided that, following separation, the osmolarity was reduced gradually. The various cell types were identified microscopically and by 3H-thymidine labelling with similar results. The present technique has demonstrated that significant variations in cell density occur during spermatogenesis. Approximately ten-fold enrichments of nearly all testis cell types were achieved by equilibrium density separation of testis cell suspensions. More homogeneous cell populations were prepared by density gradient centrifugation of cell fractions obtained from velocity sedimentation separations. Overall enrichments of spermatogonia, by 29-fold; pachytene spermatocytes, 45-fold; dividing meiotic cells, 170-fold; round spermatids, 30-fold; step 11–13 elongating spermatids, 12-fold; Leydig cells, 70-fold; and cytoplasmic fragments, 55-fold, were obtained. In this study, a method for preparation of cell suspensions was also developed to produce higher yields of spermatogonia and young primary spermatocytes; however, the density distribution of these cells was altered. 相似文献
70.