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961.
Although conspicuous courtship displays are an effective way of attracting the attention of receptive females, they could provide valuable information to rival males on the location of these females. In fiddler crabs, males that see a receptive female wave their single, greatly enlarged claw in a highly conspicuous courtship display. We test whether other males use this courtship display to alert them to the presence of receptive females that they cannot directly see. We show that male fiddler crabs (Uca mjoebergi) eavesdrop on the courtship displays of nearby males to detect mate-searching females. This allows males to begin waving before a female becomes visible. Furthermore, males appear to adjust their waving according to the information available: eavesdropping males wave 12 times faster than non-courting males but only 1.7 times slower than males in full visual contact with the female. 相似文献
962.
963.
Organ fusion and defective cuticle function in a lacs1
lacs2 double mutant of Arabidopsis 总被引:2,自引:0,他引:2
As the outermost layer on aerial tissues of the primary plant body, the cuticle plays important roles in plant development
and physiology. The major components of the cuticle are cutin and cuticular wax, both of which are composed primarily of fatty
acid derivatives synthesized in the epidermal cells. Long-chain acyl-CoA synthetases (LACS) catalyze the formation of long-chain
acyl-CoAs and the Arabidopsis genome contains a family of nine genes shown to encode LACS enzymes. LACS2 is required for cutin biosynthesis, as revealed by previous investigations on lacs2 mutants. Here, we characterize lacs1 mutants of Arabidopsis that reveals a role for LACS1 in biosynthesis of cuticular wax components. lacs1 lacs2 double-mutant plants displayed pleiotropic phenotypes including organ fusion, abnormal flower development and reduced seed
set; phenotypes not found in either of the parental mutants. The leaf cuticular permeability of lacs1 lacs2 was higher than that of either lacs1 or lacs2 single mutants, as determined by measurements of chlorophyll leaching from leaves immersed in 80% ethanol, staining with
toluidine blue dye and direct measurements of water loss. Furthermore, lacs1 lacs2 mutant plants are highly susceptible to drought stress. Our results indicate that a deficiency in cuticular wax synthesis
and a deficiency in cutin synthesis together have compounding effects on the functional integrity of the cuticular barrier,
compromising the ability of the cuticle to restrict water movement, protect against drought stress and prevent organ fusion. 相似文献
964.
Julio Aires Patricia Anglade Fabienne Baraige Monique Zagorec Marie-Christine Champomier-Vergès Marie-José Butel 《BMC microbiology》2010,10(1):29
Background
Bifidobacteria are natural inhabitants of the human gastrointestinal tract. In full-term newborns, these bacteria are acquired from the mother during delivery and rapidly become the predominant organisms in the intestinal microbiota. Bifidobacteria contribute to the establishment of healthy intestinal ecology and can confer health benefits to their host. Consequently, there is growing interest in bifidobacteria, and various strains are currently used as probiotic components in functional food products. However, the probiotic effects have been reported to be strain-specific. There is thus a need to better understand the determinants of the observed benefits provided by these probiotics. Our objective was to compare three human B. longum isolates with the sequenced model strain B. longum NCC2705 at the chromosome and proteome levels. 相似文献965.
966.
Carmen Martínez José A Molina Hortensia Alonso-Navarro Félix J Jiménez-Jiménez José AG Agúndez Elena García-Martín 《BMC neurology》2010,10(1):71
Background
Human serum paraoxonase 1 (PON1) plays a major role in the metabolism of several organophosphorus compounds. The enzyme is encoded by the polymorphic gene PON1, located on chromosome 7q21.3. Aiming to identify genetic variations related to the risk of developing brain tumors, we investigated the putative association between common nonsynonymous PON1 polymorphisms and the risk of developing astrocytoma and meningioma. 相似文献967.
968.
Leblanc DM Wood CM Fudge DS Wright PA 《Physiological and biochemical zoology : PBZ》2010,83(6):932-949
The euryhaline, amphibious mangrove killifish Kryptolebias marmoratus is known to survive weeks out of water in moist environments. We tested the hypothesis that the skin is a site of osmo- and ionoregulation in K. marmoratus. We predicted that under terrestrial conditions, gill and skin remodeling would result in an enhanced role for skin and a diminished role for the gills in osmo- and ionoregulation. Fish were exposed to water-either freshwater (FW, 1‰) or hypersaline water (saltwater [SW], 45‰)-or air over a moist surface of FW or SW for 9 d and then recovered in water. When fish were emersed for 9 d, (22)Na and (3)H-H(2)O were exchanged across the cutaneous surface. Homeostasis of whole-body Cl(-) and water levels but not of Na(+) levels was maintained over 9 d in air. In air-exposed fish, there was a significant increase in the size of skin ionocytes (in SW), a decrease in the number of skin mucous cells (in SW), and an increase in the gill interlamellar cell mass relative to those of fish in water. Gill ionocytes were mostly embedded away from the external surface in air-exposed fish, but the number and size of ionocytes increased (in FW). Interestingly, skin ionocytes formed distinct clusters of 20-30 cells. The estimated number of ionocytes over the whole skin surface was comparable to that in the gills. Overall, the findings support the hypothesis that the skin is a site of osmo- and ionoregulation in K. marmoratus in aquatic and terrestrial environments. Reversible cellular and morphological changes to the skin and gills during air exposure probably enhanced the cutaneous contribution to ion and water balance. 相似文献
969.
Cristián Tapia Sergio Molina Alvaro Diaz Lilian Abugoch Mario Diaz-Dosque Fernando Valenzuela Mehrdad Yazdani-Pedram 《AAPS PharmSciTech》2010,11(3):1294-1305
The effect of chitosan as internal or external coating on the mesalamine (5-ASA) release from calcium alginate microparticles (CaAl) was studied, and a delayed release of 5-ASA system intended for colonic drug delivery was developed. The external chitosan coating was developed by immersion of wetted CaAl in chitosan solution and the internal coating by mixing 5-ASA with chitosan solution and drying before the preparation of CaAl. Both systems were coated with Acryl-EZE® using combined fluid bed coating and immersion procedure. The results showed that in phosphate medium (pH 7.5), chitosan as 5-ASA coating promotes a quick erosion process accelerating drug release, but chitosan as external coating (CaAlCS) does not increase the T 50 value compared with the microparticles without chitosan (CaAl). Chitosan as internal or external coating was not effective to avoid the quick 5-ASA release in acidic medium (pH 1.2). The presence of β-glucosidase enzymes increases significantly the 5-ASA release for CaAl, while no effect was observed with chitosan as internal or external coating. Fourier transform infrared spectroscopy, thermogravimetric analysis, and X-ray data revealed that 5-ASA did not form a solid solution but was dispersed in the microparticles. The Acryl-EZE® coating of microparticles was effective because all the formulations showed a low release, less than 15%, of 5-ASA in acid medium at pH 1.2. Significant differences in the percentage of 5-ASA released between formulations were observed in phosphate buffer at pH 6.0. In phosphate buffer at pH 7.2, all the formulations released 100% of 5-ASA. 相似文献
970.
Zelieann R Craig John R Davis Samuel L Marion Jennifer K Barton Patricia B Hoyer 《Comparative medicine》2010,60(1):10-17
Ovarian cancer is associated with high mortality due to its late onset of symptoms and lack of reliable screening methods for early detection. Furthermore, the incidence of ovarian cancer is higher in postmenopausal women. Mice rendered follicle-depleted through treatment with 4-vinylcyclohexene diepoxide (VCD) are a model of ovary-intact menopause. The present study was designed to induce ovarian neoplasia in this model by treating mice with 7,12-dimethylbenz[a]anthracene (DMBA). Female B6C3F1 mice (age, 28 d) received intraperitoneal sesame oil (vehicle; VCD– groups) as a control or VCD (160 mg/kg; VCD+ groups) daily for 20 d to cause ovarian failure. Four months after the onset of dosing, mice from each group received a single injection of DMBA (VCD–DMBA+ and VCD+DMBA+ groups, n = 15 per group) or vehicle control (VCD–DMBA–, n = 15; VCD+ DMBA–, n = 14) under the bursa of the right ovary. Ovaries were collected 3 or 5 mo after injection and processed for histologic evaluation. Immunohistochemistry was used to confirm classification of neoplasms. None of the animals in the VCD–DMBA– and VCD–DMBA+ groups (that is, mice still undergoing estrus) had tumors at either time point. At the 3-mo time point, 12.5% of the VCD+DMBA+ mice had ovarian tumors; at 5 mo, 57.1% of the VCD+DMBA+ and 14.3% of VCD+DMBA– ovaries had neoplasms. Neoplasms stained positively for inhibin α (granulosa cells) and negatively for keratin 7 (surface epithelium), thus confirming classification of the lesions as Sertoli–Leydig cell tumors. These findings provide evidence for an increased incidence of DMBA-induced ovarian neoplasms in the ovaries of follicle-depleted mice compared with that in age-matched cycling controls.Abbreviations: DMBA, 7,12-dimethylbenz[a]anthracene; OSE, ovarian surface epithelium; VCD, 4-vinylcyclohexene diepoxideApproximately 20,000 women are diagnosed with ovarian cancer annually, of whom 15,000 are anticipated to die of the disease. Ovarian cancer ranks fifth in deaths by all cancers and first in cancers of the reproductive system.12 The survival rate of ovarian cancer patients improves greatly when the disease is detected early,2 but fewer than 20% of ovarian cancers are found at an early stage due to the lack of reliable screening methods for early detection. Because approximately two-thirds of ovarian cancer cases are diagnosed in women older than 55 y, the incidence of ovarian cancer is increased in peri- and postmenopausal women.12 For this reason, research using relevant animal models of menopause is needed to advance the understanding of the biology of neoplasms in the postmenopausal ovary.Ovarian cancer can be due to transformation of surface epithelial cells, germ cells, or sex cord and stromal cells. Almost 90% of all ovarian cancers are thought to be derived from the flat-to-cuboidal epithelial cells (that is, the ovarian surface epithelium [OSE]) that cover the ovary.6,49 Alternatively, fewer than 5% of ovarian cancers are classified as sex cord–stromal tumors, which include granulosa cell tumors, and Sertoli–Leydig cell tumors.18 The incidence of sex cord–stromal ovarian cancers is highest in women older than 50 y, but has also been diagnosed in premenopausal women.18 The etiology of ovarian cancer is not completely understood, but factors associated with development of the disease include ovulation, altered levels of gonadotropins (luteinizing and follicle-stimulating hormones) and steroid hormones (estrogens and androgens), germ-cell or follicle depletion, altered expression of oncogenes and tumor suppressor genes, altered levels of growth factors and cytokines, and exposure to environmental agents.41Recently, an ovary-intact mouse model of menopause was developed by using the occupational chemical 4-vinylcyclohexene diepoxide (VCD).24,25,27 Repeated daily dosing of mice and rats with VCD selectively destroys ovarian primordial and primary follicles by accelerating the natural process of follicular atresia.14,15,42,44 Because VCD does not target larger follicles, the animal continues to ovulate normally until no more follicles can be recruited. Thus, ovarian follicular depletion in the VCD-treated mouse is gradual. As with women undergoing perimenopause, VCD-treated mice show increased levels of follicle-stimulating hormone,27 irregular estrous cycles, and declining levels of estrogen24 as they become follicle-depleted. In addition, residual ovarian tissue is retained after ovarian failure. Therefore, preservation of residual ovarian tissue in the VCD-treated follicle-depleted mouse makes this model ideal for studying the physiology of the postmenopausal ovary. The VCD-treated mouse model of peri- and postmenopause has been used to study several menopause-related disorders including atherosclerotic lesion development,28 diabetic kidney disease,20 osteoporosis,51 and metabolic syndrome.39 Because the VCD-treated mouse has been shown to be relevant for studies related to both perimenopausal and postmenopausal stages,50 it is a useful candidate for studies of ovarian cancer.Even though spontaneous ovarian tumors in rodents have been reported,36 the paucity of these cases precludes their use in modeling ovarian cancer. Therefore, much effort has been put into developing relevant animal models for ovarian cancers. One such model involves the use of the carcinogen 7,12-dimethylbenz[a]anthracene (DMBA),8,21,23,43 a polycyclic aromatic hydrocarbon that induces carcinogenic mutations by forming DNA adducts.9 Recently, the DMBA model of carcinogenesis has been combined with the VCD model of menopause to cause ovarian cancer in F344 rats.13,19 However, no studies have characterized the combined use of both chemicals in mice. Developing this combined model in mice is important because of the existence of various genetically engineered mice that have potential relevance to enhancing our understanding of the biology of ovarian cancer.The present study was designed to determine whether ovarian failure affects susceptibility to the development of ovarian neoplasms in mice and to model DMBA-induced ovarian neoplasia in VCD-treated follicle-depleted mice. VCD-treated follicle-depleted mice and cycling controls received ovarian injections with DMBA to induce neoplasia. The incidence of neoplasms was determined by histologic evaluation, and the lesions were classified through immunostaining for keratin 7 and inhibin α. 相似文献