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排序方式: 共有265条查询结果,搜索用时 15 毫秒
101.
A series of eight new carboxylate complexes of the general type (L)nMOC(O)R (L=PMe3; n=1; M=Ag, Au; R=C2F5. L=PPh3; n=1-3; M=Ag; R=C2F5, t-Bu) have been prepared in high yields. Crystal and molecular structures have been determined for three representative examples. The crystal structure of (Ph3P)AgOC(O)C2F5 contains dimers in which the silver atoms are bridged by the carboxylate oxygen atoms. This bridging resembles the structural motif found in silver carboxylates without ligand support. Usage of the smaller phosphine PMe3 leads to the formation of a polymeric chain structure in (Me3P)AgOC(O)C2F5 with bridging carboxylate anions and short Ag-Ag contacts holding the monomers together. The reaction of (4-Me2N-C6H4)Ph2 PAuCl with two equivalents of C2F5CO2Ag leads to the formation of a mixed metal product containing both gold and silver. The crystal structure analysis of this compound revealed a tetranuclear complex containing a central dimeric silver pentafluoropropionate unit which is chelated by the (triarylphosphine)gold(I) pentafluoropropionate molecules via Ag-Au metallophilic contacts and Ag-O donor/acceptor interactions. 相似文献
102.
Vimentin Dephosphorylation by Protein Phosphatase 2A Is Modulated by the Targeting Subunit B55 总被引:6,自引:0,他引:6
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Patric Turowski Timothy Myles Brian A. Hemmings Anne Fernandez Ned J. C. Lamb 《Molecular biology of the cell》1999,10(6):1997-2015
The intermediate filament protein vimentin is a major phosphoprotein in mammalian fibroblasts, and reversible phosphorylation plays a key role in its dynamic rearrangement. Selective inhibition of type 2A but not type 1 protein phosphatases led to hyperphosphorylation and concomitant disassembly of vimentin, characterized by a collapse into bundles around the nucleus. We have analyzed the potential role of one of the major protein phosphatase 2A (PP2A) regulatory subunits, B55, in vimentin dephosphorylation. In mammalian fibroblasts, B55 protein was distributed ubiquitously throughout the cytoplasm with a fraction associated to vimentin. Specific depletion of B55 in living cells by antisense B55 RNA was accompanied by disassembly and increased phosphorylation of vimentin, as when type 2A phosphatases were inhibited using okadaic acid. The presence of B55 was a prerequisite for PP2A to efficiently dephosphorylate vimentin in vitro or to induce filament reassembly in situ. Both biochemical fractionation and immunofluorescence analysis of detergent-extracted cells revealed that fractions of PP2Ac, PR65, and B55 were tightly associated with vimentin. Furthermore, vimentin-associated PP2A catalytic subunit was displaced in B55-depleted cells. Taken together these data show that, in mammalian fibroblasts, the intermediate filament protein vimentin is dephosphorylated by PP2A, an event targeted by B55. 相似文献
103.
Sam Erlinge Kjell Danell Peter Frodin Dennis Hasselquist Patric Nilsson Eva-Britt Olofsson Mikael Svensson 《Oecologia》1999,119(4):493-500
The synchrony of Siberian lemming (Lemmus sibiricus L.) population dynamics was investigated during a ship-borne expedition along the Palaearctic tundra coast in the summer
of 1994. On 12 sites along the coast from the Kola Peninsula to Wrangel Island, relative densities of lemmings were recorded
using a standardised snap-trapping programme. The phase position of the lemming cycle in each of the studied populations was
determined based on current density estimates, signs of previous density and the age profile of each population (ageing based
on eye lens mass). In addition, dendrochronological methods were used to determine when the last peak in the density of microtine
populations occurred at each site. The examined lemming populations were in different phases of the lemming cycle. Some populations
were in the peak phase, as indicated by high current densities, an age profile in which older individuals were well represented,
and signs of high previous density (abundant old lemming faeces). Other populations were in the decline phase, as reflected
in a moderate current density, a predominance of older individuals and signs of high previous density. Populations in the
low phase had an extremely low current density and showed signs of high previous density, while populations in the increase
phase had a moderate current density, a predominance of younger individuals and showed signs of low previous density. The
results of phase determinations based on dendrochronological methods support the findings based on lemming demography. Recent
Russian studies carried out on some of the sites also agreed with our phase determination results. Thus, on a regional scale
(across the whole Palaearctic tundra), the population dynamics of Siberian lemmings can be considered asynchronous. However,
sites situated adjacent to each other were often phase synchronous, suggesting a more fine-grained pattern of dynamics with
synchrony over distances as long as 1000 km or so, e.g. the Yamal and Taymyr Peninsulas.
Received: 19 August 1998 / Accepted: 1 March 1999 相似文献
104.
PMN elastases: a comparison of the specificity of human isozymes and the enzyme from other species toward substrates and inhibitors 总被引:1,自引:0,他引:1
B G Green H Weston B M Ashe J Doherty P Finke W Hagmann M Lark J Mao A Maycock V Moore 《Archives of biochemistry and biophysics》1991,286(1):284-292
The human elastases isolated from polymorphonuclear neutrophils (PMN) and purulent sputum displayed identical kinetic constants toward substrates and inhibitors. The elastases from the two sources yield identical N-terminal sequences and were recognized by antiserum prepared against human sputum elastase (HSE) isozyme-4 (I-4). The data support the proposal put forth by Twumasi and Liener (1977, J. Biol. Chem. 252, 1917-1926) that the human elastase from sputum is of PMN origin. PMN elastases from other species displayed kinetic constants toward both substrates and inhibitors significantly different from the human enzyme. Therefore, extrapolation of inhibitor profiles from these elastases to the human source should be avoided. Four groups of isozymes were resolved from HSE by FPLC. Only the most basic isozyme (I-4) was obtained as a single species. The isozymes displayed identical macroscopic kinetic constants toward several substrates and two classes of inhibitors. The similar partition ratios observed with a cephalosporin-derived inhibitor suggest that the microscopic rate constants are also identical. The data support the proposal suggested by Baugh and Travis (1976, Biochemistry 15, 836-841) that HLE isozymes differ only in carbohydrate content. Whatever the source of human PMN elastase heterogeneity, it does not result in heterogeneous catalytic properties. In addition, a new protein was identified in elastase preparations derived from human sputum. This protein displayed homology to serine proteases and properties suggesting that it is identical to azurocidin. 相似文献
105.
106.
Patchy recruitment patterns in marine invertebrates: a spatial test of the density-dependent hypothesis in the bivalve Spisula ovalis 总被引:3,自引:0,他引:3
Density-dependent and density-independent processes have been shown to influence the population dynamics of marine invertebrates,
especially recruitment. However, their relative importance has not been evaluated in natural populations. High adult densities
have been suggested to inhibit recruitment, especially in suspension-feeders which may ingest incoming larvae. Age structure
and juvenile abundance were investigated in the bivalve Spisula ovalis in order to evaluate the importance of density dependence in generating spatial patterns. Age structure is readily established
in this species owing to annual shell lines. An extensive sample (from about 100 sites a few hundred meters apart over 4 consecutive
years) was analyzed in the statistical framework of spatial analyses, avoiding spurious correlations due to non-independence
between neighboring sites. The area studied supports about ten annual cohorts, though only a few occur at each site. The overall
picture is a mosaic of kilometer-scale patches of contrasted age structures, as revealed by highly significant spatial autocorrelations.
To our knowledge, such large-scale spatial patterns in age structure have not previously been described in benthic invertebrates.
Strong patterns are detected even for juveniles, and are independent of the adult biomass present before settlement. Therefore,
patchy patterns of age structure mainly reflect density-independent effects, such as spatial variations in larval supply,
passive transport of juveniles, or predation on recruits. In the absence of detailed spatial analyses, such patterns have
been misinterpreted previously as negative effects of adult density on settlement success.
Received: 21 November 1996 / Accepted: 20 February 1997 相似文献
107.
Effect of Resistance to Chloramphenicol on Bacteriophage Sensitivity of Group A Streptococci 总被引:1,自引:0,他引:1
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Several phage hosts of group A streptococci became resistant to lysis by bacteriophage as a consequence of having acquired the ability to grow in the presence of chloramphenicol. The phage was adsorbed to the streptococcal cell, and P(32)-labeling of the phage showed that the phage genome penetrated the chloramphenicol (CM)- resistant cells as it did the parent cells. However, artificial lysis of the infected CM-resistant cells with chloroform or enzymes revealed no intracellular mature phage particles. Lysates of infected CM-resistant cells contained no phage-related antigenic materials which possessed serum-blocking power, although they were readily detected in lysates of infected parent cells. The CM-resistant cells were not lysogenized by the phage. Only cells resistant to more than 10 mug/ml of chloramphenicol were resistant to phage, and this threshold effect was taken as an indication of at least two different loci of chloramphenicol resistance on the streptococcal genome. Strains resistant to high levels of other antibiotics, such as streptomycin and erythromycin, showed no resistance to lysis by phage. Evidence indicated that the mutant cells were deficient in an essential function associated with the phage genome. 相似文献
108.
109.
110.
Uptake, production and metabolism of cysteinyl leukotrienes in the isolated perfused rat liver. Inhibition of leukotriene uptake by cyclosporine. 总被引:3,自引:0,他引:3
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1. The isolated perfused rat liver efficiently takes up cysteinyl leukotrienes (LTs) C4, D4, E4 and N-acetyl-LTE4 from circulation. More than 70% of these cysteinyl LTs are excreted from liver into bile within 1 h of onset of a 5 min infusion, while about 5% remain in the liver. About 20% of infused N-acetyl-LTE4 escapes hepatic first-pass extraction under our conditions. 2. Metabolites of LTC4 appearing in bile within 20 min of the onset of infusion include mainly LTD4 and N-acetyl-LTE4, but also omega-hydroxy-N-acetyl-LTE4 and omega-carboxy-N-acetyl-LTE4. Metabolites generated from omega-carboxy-N-acetyl-LTE4 by beta-oxidation from the omega-end represent the major biliary LTs secreted at later times. 3. Stimulation of the isolated perfused liver by the combined infusion of the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA) and the Ca2+ ionophore A23187 results in a transient increase of endogenous cysteinyl LT production, which is independent of extrahepatic cells. 4. The immunosuppressive drug cyclosporine causes a dose-dependent inhibition of hepatobiliary cysteinyl LT excretion, probably by interference with the sinusoidal uptake system for cysteinyl LTs. 相似文献