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101.
Long-distance transport of many organelles inside eukaryotic cells is driven by the dynein and kinesin motors on microtubule filaments. More than 30 years since the discovery of these motors, unanswered questions include motor–organelle selectivity, structural determinants of processivity, collective behaviour of motors and track selection within the complex cytoskeletal architecture, to name a few. Fluorescence microscopy has been invaluable in addressing some of these questions. Here we present a review of some efforts to understand these sub-microscopic machines using fluorescence. 相似文献
102.
Dharam Pal Pathak Vidushi Sharma Sachin Kumar 《Bioorganic & medicinal chemistry letters》2018,28(9):1566-1569
Coumarin containing pyrazoline derivatives have been synthesized and tested as inhibitors of in vitro development of a chloroquine-sensitive (MRC-02) and chloroquine-resistant (RKL-2) strain of Plasmodium falciparum and in vivo Plasmodium berghei malaria. Docking study was also done on cysteine protease falcipain-2 which showed that the binding pose of C-14 molecule and epoxysuccinate, inhibitor of falcipain-2, binds in the similar pattern. The most active antimalarial compound was 3-(1-benzoyl-5-(4-flurophenyl)-4,5-dihydro-1H-pyrazol-3yl)-7-(diethyamino)-2H-chromen-2-one C-14, with an IC50 of 4.21?µg/ml provided complete protection to the infected mice at 24?mg/kg X 4?days respectively. 相似文献
103.
Natural infection of Aspergillus flavus was observed in adults of Blattella germanica. Though the adult insects exhibited
no external symptoms, they became hypoactive and later died. The dead and experimentally infected insects repeatedly yielded
Aspergillus flavus in culture on Czapek's medium. Direct microscopic observation of the tissues of infected insects revealed
fungal material. The blood films stained with Giemsa stain showed granulocytes (GRs) engulfing fungal hyphae. A remarkable
increase in GR and plasmatocyte (PL) counts occurred in differential haemocyte counts (DHCs) of the infected insects. Two
main types of immunological responses of the insect noticed were phagocytosis and encapsulation. DHC showed maximum involvement
of GRs and PLs in immune mechanism.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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107.
Loss of DNA following C-banding procedures 总被引:3,自引:0,他引:3
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109.
Sangeeta Mandal J. K. Jena Rajeev K. Singh Vindhya Mohindra W. S. Lakra Geetanjali Deshmukhe Abhinav Pathak Kuldeep K. Lal 《Molecular biology reports》2016,43(2):91-98
The stock characterization of wild populations of Silonia silondia is important for its scientific management. At present, the information on genetic parameters of S. silondia is very limited. The species-specific microsatellite markers were developed in current study. The validated markers were used to genotype individuals from four distant rivers. To develop de novo microsatellite loci, an enriched genomic library was constructed for S. silondia using affinity–capture approach. The markers were validated for utility in population genetics. A total number of 76 individuals from four natural riverine populations were used to generate data for population analysis. The screening of isolated repeat sequences yielded eleven novel polymorphic microsatellite loci. The microsatellite loci exhibited high level of polymorphism, with 6–24 alleles per locus and the PIC value ranged from 0.604 to 0.927. The observed (Ho) and expected (He) heterozygosities ranged from 0.081 to 0.84 and 0.66 to 0.938, respectively. The AMOVA analysis indicated significant genetic differentiation among riverine populations (overall FST = 0.075; P < 0.0001) with maximum variation (92.5 %) within populations. Cross-priming assessment revealed successful amplification (35–38 %) of heterologous loci in four related species viz. Clupisoma garua, C. taakree, Ailia coila and Eutropiichthys vacha. The results demonstrated that these de novo polymorphic microsatellite loci are promising for population genetic variation and diversity studies in S. silondia. Cross-priming results indicated that these primers can help to get polymorphic microsatellite loci in the related catfish species of family Schilbidae. 相似文献
110.
Asha S. Multani Mustafa Ozen Alpana Agrawal Vicki L. Hopwood Andrew C. Von Eschenbach Sen Pathak 《In vitro cellular & developmental biology. Animal》1999,35(4):236-239
Summary Conventional and molecular cytogenetic analyses of three murine cancer cell lines that had been induced in male athymic mice
by the injection of three different human prostate cancer cell lines revealed selective amplification of the Y chromosome.
In particular, analysis of metaphase and interphase nuclei by fluorescence in situ hybridization (FISH) with the mouse Y chromosome-specific
DNA painting probe revealed the presence of various numbers of Y chromosomes, ranging from one to eight, with a large majority
of nuclei showing two copies (46.5–60.1%). In Interphase nuclei, the Y chromosomes showed distinct morphology, allowing identification
irrespective of whether the preparations were treated for 15 min or for 5 h with Colcemid, a chemical known to cause chromosome
condensation. However, FISH performed on human lymphocyte cultures with chromosome-specific DNA painting probes other than
the Y chromosome did not reveal condensed chromosome morphology in interphase nuclei even after 12 h of Colcemid treatment.
Our FISH results indicate that (1) the Y chromosome is selectively amplified in all three cell lines; (2) the mouse Y chromosome
number is comparable in both interphase and metaphase cells; (3) the Y chromosome number varies between one and eight, with
a large majority of cells showing two or three copies in most interphase nuclei; (4) the condensation of the Y chromosome
is not affected by the duration of Colcemid treatment but by its inherent DNA constitution; and (5) the number of copies of
the Y chromosome is increased and retained not only in human prostate tumor cell lines but also in murine tumors induced by
these prostate tumor cell lines. 相似文献