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31.
R Welbourn G Goldman L Kobzik I Paterson C R Valeri D Shepro H B Hechtman 《Journal of immunology (Baltimore, Md. : 1950)》1990,145(6):1906-1911
Neutrophils and eicosanoid chemoattractants are centrally involved with ischemia-reperfusion (I/R) injury. The CD 18 complex of adhesive glycoproteins, readily up-regulated by chemoattractants in vitro, is required for polymorphonuclear leukocyte (PMN) adherence to endothelium. This study tests whether CD 18 is up-regulated by ischemia in vivo and its role in mediating PMN diapedesis. Anesthetized rabbits underwent 3 h of bilateral hindlimb tourniquet ischemia (n = 16). Ten min after tourniquet release, levels of plasma leukotriene (LT)B4 increased to 390 +/- 62 pg/ml (mean +/- SE), higher than 134 +/- 26 pg/ml in control rabbits (n = 13, p less than 0.01). Aliquots of plasma were added to whole blood from normal rabbits (n = 6) for flow cytometric analysis of neutrophils with the CD 18 mAb R 15.7. Addition of I/R plasma failed to demonstrate an increase in surface expression of CD 18. Similarly, no CD 18 up-regulation was observed in vivo upon reperfusion in ischemic animals pretreated with mAb R 15.7 (n = 3). However, I/R plasma when introduced into plastic chambers taped atop dermabrasion sites in normal rabbits (n = 12) resulted in diapedesis, measured by the accumulation after 3 h of 1130 +/- 125 PMN/mm3 in the chambers relative to 120 +/- 31 PMN/mm3 with control plasma (p less than 0.01). Diapedesis in response to I/R plasma was abolished by pretreatment with mAb R 15.7 (less than 5 PMN/mm3, n = 6), was reduced by U 75,302, an LTB4 receptor antagonist (253 +/- 101 PMN/mm3, n = 6) (both p less than 0.01) and was not protein synthesis dependent. These results demonstrate that PMN diapedesis in response to I/R plasma is exclusively dependent upon the CD 18 glycoprotein complex by an LTB4-dependent mechanism, despite the fact that CD 18 is not up-regulated on circulating PMN in ischemia. These data indirectly indicate the functional importance of conformational changes of CD 18 in determining PMN adhesion. 相似文献
32.
The beta actin promoter. High levels of transcription depend upon a CCAAT binding factor 总被引:10,自引:0,他引:10
W W Quitschke Z Y Lin L DePonti-Zilli B M Paterson 《The Journal of biological chemistry》1989,264(16):9539-9546
33.
Transforming growth factor-alpha in the mammalian brain. Immunohistochemical detection in neurons and characterization of its mRNA 总被引:5,自引:0,他引:5
J E Kudlow A W Leung M S Kobrin A J Paterson S L Asa 《The Journal of biological chemistry》1989,264(7):3880-3883
In this communication, we demonstrate that adult mammalian brain neurons express transforming growth factor-alpha (TGF-alpha). We used the anti-TGF-alpha monoclonal antibody, MF9, to immunohistochemically localize TGF-alpha in human and rat brain. We found specific immunoreactivity in neurons throughout the brain which was not a result of cross-reactivity of MF9 with the neuropeptide, synenkephalin. Northern blot analysis of bovine and rat brain RNA using human and rat TGF-alpha cDNA probes, respectively, revealed a single 4.8-kilobase pair mRNA with approximately equal abundance in the bovine brainstem, cerebellum, hypothalamus, and cerebral cortex. Fetal rat brain had about 2-fold more TGF-alpha mRNA than did adult rat. The brain TGF-alpha cDNA was cloned from a human neonatal brainstem library. Four identical clones were isolated after screening 10(6) recombinant lambda gt11 phage. The sequence of the 894-base pair cDNA was virtually identical with the cDNA isolated from a human renal cell carcinoma. A single alanine codon was deleted in the brain cDNA at an exon-exon junction. The alanine deletion is within the amino-terminal region of the TGF-alpha precursor that is thought to be removed by proteolytic processing of the precursor to the mature growth factor. These studies indicate that the normal mammalian brain neurons express TGF-alpha. 相似文献
34.
The effect of dicyclohexylcarbodiimide (DCCD) on electron transfer in the acceptor quinone complex of reaction centers (RC) from Rhodobacter sphaeroides is reported. DCCD covalently labelled the RC over a wide concentration range. At low concentrations (<10 M) the binding was specific for the L subunit. At relatively high concentrations (>100 M) DCCD accelerated the rate of charge recombination of the P+QB
- state, consistent with a decrease in the equilibrium constant between QA
-QB and QAQB
-. At similar concentrations, in the presence of cytochrome c as exogenous donor, turnover of the RC was inhibited such that only three cytochromes were oxidized in a train of flashes. Both these inhibitory effects were fully reversed by dialysis, indicating that stable covalent binding was not involved. Possible mechanisms of action are discussed in terms of the putative role of specific residues in proton transfer and protonation and release of quinol from the RC. 相似文献
35.
J. D. Paterson 《International journal of primatology》1996,17(4):585-611
I analyzed somatometric measurements from subsets of the Texas and Oregon transplanted troops of Japanese macaques(Macaca fuscata) to reveal secular changes in body size and shape. Body weights of the Texas population (N = 59) are lower than those of the
Oregon population(N = 49) and the founding population from Arashiyama. The adult weights of the Oregon population are significantly higher than the
founding population from Mihara. There are significant differences in adult circumferential measures and in skinfolds, which
are correlated with the increased weight of the Oregon macaques. The adult Texas macaques have longer limb segments in comparison
with the adult Oregon troop members, while the latter have significantly longer heads and trunks. Examination of the developing
morphological trends through regression analyses on the complete sample suggests distinctive growth patterns for each population.
Members of the Texas population start with smaller initial measurements but hold a steeper growth pattern for limb segments,
while the Oregon macaques start larger in most measures and show lower growth rates. I argue that these differences in both
somatometry and growth patterns are related to the differing climatic conditions under which the translocated macaques have
lived. This set of analyses supports the basic arguments for Bergmann’s rule and Allen’s rule. 相似文献
36.
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38.
Asim K. Dutta-Roy Margaret J. Gordon Derek J. Leishman Brian J. Paterson Garry G. Duthie William P. T. James 《Molecular and cellular biochemistry》1993,123(1-2):139-144
An -tocopherol-binding protein has been isolated and purified from rabbit heart cytosol. The purified protein had an apparent molecular mass of 14,200, as derived from SDS-PAGE. The content of the protein in rabbit heart was around 11.8 g per g of tissue. The binding of -tocopherol to the purified protein was rapid, reversible, and saturable. Neither nor tocopherol could displace the bound -tocopherol from the protein, suggesting a high specificity for -tocopherol. -Tocopherol-binding protein did not bind oleate. Transfer of -tocopherol from liposomes to mitochodria was stimulated 8-fold in the presence of the binding protein, suggesting that this protein may be involved in the intracellular transport of -tocopherol in the heart. 相似文献
39.
An improved method of plant megabase DNA isolation in agarose microbeads suitable for physical mapping and YAC cloning 总被引:6,自引:0,他引:6
Rod A. Wing Vipin K. Rastogi Hong-Bin Zhang rew H. Paterson Steven D. Tanksley 《The Plant journal : for cell and molecular biology》1993,4(5):893-898
The isolation of high quality megabase DNA from plant cells that is susceptible to a variety of molecular reagents is a critical first step in the physical analysis of complex genomes. A method for the isolation of such DNA by encapsulating plant protoplasts in agarose microbeads is presented. In comparison with the conventional agarose plug method, microbeads provide a dramatic increase in the surface area yielding megabase DNA that can be treated essentially as an aqueous DNA solution. Examples of the utility of DNA prepared by this technique for physical mapping, partial restriction enzyme digestion and cloning of large inserts as YACs are presented. 相似文献
40.
A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis 总被引:13,自引:0,他引:13
Andrew H. Paterson Curt L. Brubaker Jonathan F. Wendel 《Plant Molecular Biology Reporter》1993,11(2):122-127
Extraction of high-quality genomic DNA fromGossypium (cotton) species is difficult due to high levels of polysaccharide, oxidizable quinones, and other interfering substances.
We describe a procedure that consistently permits isolation of cotton genomic DNA of satisfactory size and quality for RFLP
and PCR analysis, as well as for most routine cloning applications. Several antioxidants, phenol-binding reagents, and phenol
oxidase inhibitors are used throughout the procedure, and most polysaccharides are eliminated early in the procedure by isolation
of nuclei. 相似文献