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91.
Maykon Passos Cristiano Danon Clemes CardosoTânia Maria Fernandes-Salomão 《Comptes rendus biologies》2014,337(2):78-85
The incorporation of fragments of mitochondrial DNA (mtDNA) in the nuclear genome, known as numts (nuclear mitochondrial pseudogenes), undermines general assumptions concerning the use of mtDNA in phylogenetic and phylogeographic studies. Accidental amplifications of these nuclear copies instead of the mitochondrial target can lead to crucial misinterpretations, thus the correct identification of numts and their differentiation from true mitochondrial sequences are important in preventing this kind of error. Our goal was to describe the existence of cytochrome b (cytb) numts in the leafcutter ant Acromyrmex striatus (Roger, 1863). PCR products were directly sequenced using a pair of universal primers designed to amplify the cytb gene of these insects. Other species of leafcutter ants were also sequenced. The sequences were analyzed and the numts were identified by the presence of double peaks, indels and premature stop codons. Only A. striatus clearly showed the presence of numts, while the other species displayed the expected amplification of the mtDNA cytb gene target using the same primer pair. We hope that our report will highlight the benefits and challenges of using mtDNA in the molecular phylogenetic reconstruction and phylogeographic studies of ants, while establishing the importance of numts reports for future studies. 相似文献
92.
Silaghi C Scheuerle MC Friche Passos LM Thiel C Pfister K 《Parasite (Paris, France)》2011,18(1):57-62
Central Switzerland is a highly endemic region for tick-borne fever (TBF) in cattle, however, little is known about A. phagocytophilum in goats. In the present study, 72 animals from six goat flocks (373 EDTA blood-samples) in Central Switzerland were analysed for A. phagocytophilum DNA. A real-time PCR targeting the msp2 gene of A. phagocytophilum was performed and in positive samples the partial 165 rRNA, groEL and msp4 gene were amplified for sequence analysis. Four DNA extracts were positive. Different sequence types on basis of the amplified genes were found. For comparison, sequences of A. phagocytophilum from 12 cattle (originating from Switzerland and Southern Germany) were analysed. The 165 rRNA gene sequences from cattle were all identical amongst each other, but the groEL and msp4 gene differed depending on the origin of the cattle samples and differed from the variants from goats. This study clearly provides molecular evidence for the presence of different types of A. phagocytophilum in goat flocks in Switzerland, a fact which deserves more thorough attention in clinical studies. 相似文献
93.
de Sousa AA Reis R Bento-Torres J Trévia N Lins NA Passos A Santos Z Diniz JA Vasconcelos PF Cunningham C Perry VH Diniz CW 《PloS one》2011,6(1):e15597
An enriched environment has previously been described as enhancing natural killer cell activity of recognizing and killing virally infected cells. However, the effects of environmental enrichment on behavioral changes in relation to virus clearance and the neuropathology of encephalitis have not been studied in detail. We tested the hypothesis that environmental enrichment leads to less CNS neuroinvasion and/or more rapid viral clearance in association with T cells without neuronal damage. Stereology-based estimates of activated microglia perineuronal nets and neurons in CA3 were correlated with behavioral changes in the Piry rhabdovirus model of encephalitis in the albino Swiss mouse. Two-month-old female mice maintained in impoverished (IE) or enriched environments (EE) for 3 months were behaviorally tested. After the tests, an equal volume of Piry virus (IEPy, EEPy)-infected or normal brain homogenates were nasally instilled. Eight days post-instillation (dpi), when behavioral changes became apparent, brains were fixed and processed to detect viral antigens, activated microglia, perineuronal nets, and T lymphocytes by immuno- or histochemical reactions. At 20 or 40 dpi, the remaining animals were behaviorally tested and processed for the same markers. In IEPy mice, burrowing activity decreased and recovered earlier (8-10 dpi) than open field (20-40 dpi) but remained unaltered in the EEPy group. EEPy mice presented higher T-cell infiltration, less CNS cell infection by the virus and/or faster virus clearance, less microgliosis, and less damage to the extracellular matrix than IEPy. In both EEPy and IEPy animals, CA3 neuronal number remained unaltered. The results suggest that an enriched environment promotes a more effective immune response to clear CNS virus and not at the cost of CNS damage. 相似文献
94.
Lucas M. Aguiar Rodrigo F. Moro-Rios Thiago Silvestre José E. Silva-Pereira Diego R. Bilski Fernando C. Passos 《Studies on Neotropical Fauna and Environment》2013,48(3):153-161
We described the diets of two procyonids, the brown-nosed coati Nasua nasua and the crab-eating raccoon Procyon cancrivorus, through analysis of stomach contents of road-killed specimens in southern Brazil. We compared them with previously published dietary information for another syntopic mesopredator, the crab-eating fox Cerdocyon thous. The landscape of the study area includes native grasslands, forests, exotic tree plantations, and other crops. Food items were represented by frequency of occurrence (FO) and relative volume (RV). Stomach contents of 23 coatis were analyzed. Animal and plant items were equally frequent, although the volume of plants was greater. Exotic plant species were consumed more than native plants. Among prey items, invertebrates were more important than vertebrates, mainly because of the frequency of coleopterans and annelids and the volume of necrophagous dipteran larvae. Five specimens of raccoons were analyzed, in which animal items had the highest FO and plant items had the highest RV. Both invertebrate and vertebrate prey had the same FO, although vertebrates had a higher RV. Our data suggest that these procyonids are opportunistic hypocarnivores, utilizing anthropic sources, with diet overlap. The coatis also overlapped their diet substantially with the foxes. The stomach-contents analysis revealed the importance of fruit pulp biomass, soft-bodied animals such as larvae, and also soil, which might indicate feeding habits such as scavenging and geophagy. 相似文献
95.
96.
Carlos Passos Federico Reyes Bettina Tassino Gil G. Rosenthal Andrés González 《Ethology : formerly Zeitschrift fur Tierpsychologie》2013,119(10):891-897
Chemical communication is an important mediator of social interactions in fish, including mate recognition, mate choice and intrasexual competition. We used a simultaneous‐choice design to test the response of adult females of the annual killifish, Austrolebias reicherti, towards male‐conditioned water, both crude and fractionated into its polar and non‐polar components. Females preferred male‐conditioned water when tested both against control water and female‐conditioned water. The polar and non‐polar fractions of the male‐conditioned water were not significantly attractive, although a tendency was observed towards the non‐polar fraction. Our study shows that annual fish respond to the chemical cues of potential mates. The chemical channel is likely to be particularly important in the muddy ephemeral ponds in which they occur. 相似文献
97.
98.
Mitochondria and ageing: winning and losing in the numbers game 总被引:3,自引:0,他引:3
Passos JF von Zglinicki T Kirkwood TB 《BioEssays : news and reviews in molecular, cellular and developmental biology》2007,29(9):908-917
Mitochondrial dysfunction has long been considered a key mechanism in the ageing process but surprisingly little attention has been paid to the impact of mitochondrial number or density within cells. Recent reports suggest a positive association between mitochondrial density, energy homeostasis and longevity. However, mitochondrial number also determines the number of sites generating reactive oxygen species (ROS) and we suggest that the links between mitochondrial density and ageing are more complex, potentially acting in both directions. The idea that increased density, especially when combined with mitochondrial dysfunction, might accelerate ageing is supported by a negative correlation between mitochondrial density and maximum longevity in an interspecies comparison in mammals, and by evidence for an intimate interconnection between cellular ROS levels, mitochondrial density and cellular ageing. Recent data suggest that retrograde response, which activates mitochondrial biogenesis, accompanies cellular ageing processes. We hypothesise that increased mitochondrial biogenesis, and possibly also impaired degradation and segregation of mitochondria, if occurring as adaptation to pre-existing mitochondrial dysfunction, might aggravate ROS production and thus actively contribute to ageing. 相似文献
99.
Soleimanpour-Lichaei HR Kühl I Gaisne M Passos JF Wydro M Rorbach J Temperley R Bonnefoy N Tate W Lightowlers R Chrzanowska-Lightowlers Z 《Molecular cell》2007,27(5):745-757
Human mitochondria contain their own genome, encoding 13 polypeptides that are synthesized within the organelle. The molecular processes that govern and facilitate this mitochondrial translation remain unclear. Many key factors have yet to be characterized-for example, those required for translation termination. All other systems have two classes of release factors that either promote codon-specific hydrolysis of peptidyl-tRNA (class I) or lack specificity but stimulate the dissociation of class I factors from the ribosome (class II). One human mitochondrial protein has been previously identified in silico as a putative member of the class I release factors. Although we could not confirm the function of this factor, we report the identification of a different mitochondrial protein, mtRF1a, that is capable in vitro and in vivo of terminating translation at UAA/UAG codons. Further, mtRF1a depletion in HeLa cells led to compromised growth in galactose and increased production of reactive oxygen species. 相似文献
100.
Santos Beatriz da Conceição Daniele Priscila Corrêa Diego Oliveira Passos Matheus Felipe Campos Monique Paiva Adamoski Douglas Galli-Terasawa Lygia Vitória Mariano André Bellin Vargas José Viriato Coelho Kava Vanessa Merlo 《Journal of applied phycology》2022,34(2):729-743
Journal of Applied Phycology - The present study evaluated the effect of blue, red, and white light on the expression of four carotenogenic genes (psy, lyc, bkt, and crtr-r); one gene related to... 相似文献