Striae albae: a morphological study on the human skin

Specimens of abdomen skin, comprising alternate areas of striae albae and healthy skin, were removed during surgical lipectomy from multiparous and obese women between the ages of 24 and 53 years. A flattening and thinning of the striae albae surface and the almost complete disappearance of dermal papillae was observed in paraffin and thin sections. The papillary dermis was found to be almost completely replaced by straight bundles of collagen fibres running parallel to the skin surface. Immunofluorescence data revealed in these bundles high positivity for type I collagen. The underlying reticular dermis was also found to contain large densely packed bundles of collagen fibres running parallel to the skin surface. Both papillary and reticular dermis collagen fibres were mainly arranged orthogonally to the main axis of the stria. Furthermore, the density of the collagen fibre bundles and the diameter of the collagen fibrils was found to be greater than that of the clinically healthy skin. A larger number of elastic fibres, which presented an abnormal ultrastructural appearance, were visible in pathological papillary and reticular dermis.     

Tag loss is a minor limiting factor in sea turtle tagging programs relying on distant tag returns: the case of Mediterranean loggerhead sea turtles

As in many other species, tagging has been routinely conducted for decades in over a hundred sea turtle capture-mark-recapture (CMR) programs worldwide. Tag loss is a key limiting factor because it violates the main assumption in CMR models; however, very few estimates of tag loss exist, and we provide here a review. No published estimations of tag loss are available for the Mediterranean, in spite of intensive tagging since the 1980s. This study aims to provide an estimation of tag loss in loggerhead turtles tagged in the Mediterranean. We modeled 64 tag returns out of ca. 2200 loggerhead turtles tagged at Mediterranean foraging grounds, with mark-recapture intervals up to 11.5 years, in order to estimate a daily tag loss probability of the most used tag applied to the most common turtle species in the region. Five models were evaluated through maximum likelihood estimation. The model with the best fit described a tag loss initially high and then decreasing to a lower asymptote, which is probably due to some defective tag applications. The resulting tag loss (0.15 in the first year and 0.31 after 5 years) was comparable or even lower than those from other areas and/or species and predictions indicate that double tagging can make a turtle identifiable for a long period. Hence, in our tagging program and probably in similar ones as well, tag loss appears to be the less important of the factors affecting tag returns, and efforts in other directions are more likely to improve CMR results.     

Peritoneal dialysis fluid activates calcium signaling and apoptosis in mesothelial cells

A larger diffusion of peritoneal dialysis (PD) is limited by the progressive deterioration of the dialysis membrane structure and function, characterized in vitro and in vivo by mesothelial cell loss and closely related to the use of bioincompatible dialysis solutions. The apoptosis rate of rat and human mesothelial cells incubated in commercial PD fluid (PDF, 4.25 g/dL dextrose) became significant as early as 1 h after PDF addition and reached a plateau at 4–5 h. This pattern was unchanged after exposure to 1.5 g/dL dextrose PDF or freshly prepared PDF, indicating that effects were independent on the dextrose strength and manufacturing procedures but strictly dependent on PDF composition. Molecular studies revealed that PDF exposure inactivated the physiological volume recovery from hypertonic shrinkage, accompanied by an abnormal Ca²⁺ signaling: a progressive intracellular Ca²⁺ ([Ca²⁺]_i) rise resulting from an increased Ca²⁺ entry. PDF also affected cytoskeleton integrity: early dissolution of actin filaments occurred well before the appearance of typical apoptosis features. Lastly, the PDF dependent apoptosis was almost completely prevented by the contemporary Ca²⁺ concentration decrease and K⁺ addition. This study suggests that the PDF dependent apoptosis arises from the extreme volume perturbations in mesothelial cells, turned out unable to regulate their volume back once exposed to a hyperosmolal medium containing high Ca²⁺ levels in the absence of K⁺, such PDF.     

The lethal moiety of the surface slime glycolipoprotein ofPseudomonas aeruginosa

Phenol treatment of the glycolipoprotein fromPseudomonas aeruginosa, followed by gel filtration and anion-exchange chromatography, produced a glycolipid fragment free of protein. A second, lipid-rich fragment was obtained as a precipitate of dilute acetic-acid hydrolysis of the glycolipoprotein. After solubilization, the lipid-rich fragment was processed on Sephadex G-200. Both lipid-containing fragments were immunologically homogeneous in Ouchterlony plates against rabbit antiserum to crude glycolipoprotein. The precipitin bands indicated that polysaccharide residues conferred the antigenic specificity. General chemical analyses were made on each fragment. All lipid-containing fragments were lethal, to varying degrees, on injection in mice. Proteinase K digestion of the glycolipoprotein did not reduce its lethality. All lipid-containg fragments stimulated varying levels of antibody capable of passively protecting mice against lethal challenge with viablePseudomonas aeruginosa. The contribution of the carbohydrate moiety to the expression of lethality is discussed.     

Assessing the current status of urban forest resources in the context of Parco Nord, Milan, Italy

During the early part of the 1980s, a major project called Parco Nord was undertaken by the Lombardia Region to establish forest resources within an industrial area located in the northern part of the city of Milan. Since 1983, more than 60 ha of formerly industrial land has been converted into urban forest plantations, thus creating large patches of trees with the potential to sustain a wide range of functions and services. This paper describes an integrative study aimed to assess the current status of forest resources in Parco Nord. It focuses on the actions taken to determine whether forest resources significantly changed their status 20 years after their establishment, considering historical field data and records of management practices. Analyses have been conducted at both stand and tree level by collecting quantitative and qualitative parameters. Stand-level analysis gave a quantitative estimation of the response of species to ecological conditions and management practices while tree-level analysis provided evidence of species renovation after thinning operations.     

Specific interaction between cyclophilin and cyclic peptides

Cyclophilin A, a peptidyl-prolyl cis–trans isomerase that catalyzes the otherwise slow isomerization of Xaa-Pro imidic bond, specifically binds the immunosuppressant cyclosporin A. Herein we reportevidence on binding of cyclolinopeptide A and its synthetic analogue, [Aib^5,6-D -Ala⁸] cyclolinopeptide, to bovine cyclophilin A. Binding experiments were monitored by fluorescence. CD, and second-derivative spectroscopies, evidencing no remarkable rearrangement of protein structure organization. The possibility that cyclolinopeptide A could act as a substitute of cyclosporin A in the immunosuppression modulation is also briefly discussed. © 1995 John Wiley & Sons, Inc.