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941.
942.
Jane Koskimäki Otso Huitu Janne S. Kotiaho Satu Lampila Antero Mäkelä Risto Sulkava Mikko Mönkkönen 《Population Ecology》2014,56(2):341-348
To devise effective conservation actions, it is important to know which factors are associated with the population parameters of a declining population. Using mark–recapture methods, we estimated the annual population size, growth rate and survival probability of an ear-tagged flying squirrel population over a 15-year period in a 4,500 ha study area in western Finland. The species is considered vulnerable, but detailed knowledge concerning population sizes or trends is lacking. The population parameters and changes therein were regressed against habitat availability, an indicator of predation pressure, and mean winter temperature (an indicator of climate change), to reveal potential reasons for trends in the population. The best-fit models suggested the annual growth rate to be below one, and on average it was 0.93 (±0.06; SE) across the 15-year period. The survival probability was about 0.22 (±0.03) for juveniles and 0.50 (±0.03) for adults. The population size of adult flying squirrels decreased from 65 (±11) individuals in 1995 to 29 (±6) individuals in 2009. The number of flying squirrels was associated with the amount of available habitat, but the decline in population size was more rapid than the loss of habitat area. If the current decreasing trend in habitat availability continues, the population might become extinct by the year 2020. To halt the population decline, it is necessary to refrain from clear-cutting mature spruce stands until new suitable habitats develop from the maturation of younger forests. 相似文献
943.
944.
Kathy Darragh Anna Orteu Daniella Black Kelsey J. R. P. Byers Daiane Szczerbowski Ian A. Warren Pasi Rastas Ana Pinharanda John W. Davey Sylvia Fernanda Garza Diana Abondano Almeida Richard M. Merrill W. Owen McMillan Stefan Schulz Chris D. Jiggins 《PLoS biology》2021,19(1)
Plants and insects often use the same compounds for chemical communication, but not much is known about the genetics of convergent evolution of chemical signals. The terpene (E)-β-ocimene is a common component of floral scent and is also used by the butterfly Heliconius melpomene as an anti-aphrodisiac pheromone. While the biosynthesis of terpenes has been described in plants and microorganisms, few terpene synthases (TPSs) have been identified in insects. Here, we study the recent divergence of 2 species, H. melpomene and Heliconius cydno, which differ in the presence of (E)-β-ocimene; combining linkage mapping, gene expression, and functional analyses, we identify 2 novel TPSs. Furthermore, we demonstrate that one, HmelOS, is able to synthesise (E)-β-ocimene in vitro. We find no evidence for TPS activity in HcydOS (HmelOS ortholog of H. cydno), suggesting that the loss of (E)-β-ocimene in this species is the result of coding, not regulatory, differences. The TPS enzymes we discovered are unrelated to previously described plant and insect TPSs, demonstrating that chemical convergence has independent evolutionary origins.Plants and insects often use the same compounds for chemical communication, but little is known about the convergent evolution of such chemical signals. This study identifies a novel terpene synthase involved in production of an anti-aphrodisiac pheromone by the butterfly Heliconius melpomene. This enzyme is unrelated to other insect terpene synthases, providing evidence that the ability to synthesise terpenes has arisen multiple times independently within the insects. 相似文献
945.
946.
Ingvild Aukrust Lise Bj?rkhaug Maria Negahdar Janne Molnes Bente B. Johansson Yvonne Müller Wilhelm Haas Steven P. Gygi Oddmund S?vik Torgeir Flatmark Rohit N. Kulkarni P?l R. Nj?lstad 《The Journal of biological chemistry》2013,288(8):5951-5962
Glucokinase is the predominant hexokinase expressed in hepatocytes and pancreatic β-cells, with a pivotal role in regulating glucose-stimulated insulin secretion, illustrated by glucokinase gene mutations causing monogenic diabetes and congenital hyperinsulinemic hypoglycemia. A complex tissue-specific network of mechanisms regulates this enzyme, and a major unanswered question in glucokinase biology is how post-translational modifications control the function of the enzyme. Here, we show that the pancreatic isoform of human glucokinase is SUMOylated in vitro, using recombinant enzymes, and in insulin-secreting model cells. Three N-terminal lysines unique for the pancreatic isoform (Lys-12/Lys-13 and/or Lys-15) may represent one SUMOylation site, with an additional site (Lys-346) common for the pancreatic and the liver isoform. SUMO-1 and E2 overexpression stabilized preferentially the wild-type human pancreatic enzyme in MIN6 β-cells, and SUMOylation increased the catalytic activity of recombinant human glucokinase in vitro and also of glucokinase in target cells. Small ubiquitin-like modifier conjugation represents a novel form of post-translational modification of the enzyme, and it may have an important regulatory function in pancreatic β-cells. 相似文献