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1.
A Taddeo A Renieri A Fioravanti G Morozzi E Parrini M S D'Amato M G Ricci 《Bollettino della Società italiana di biologia sperimentale》1984,60(9):1709-1714
Modifications on the binding of uric acid to human plasma proteins have been studied in regularly menstruating females aged 25-30 years with a normal cycle, in comparison with a group of healthy age-matched males and with a group of post-menopausal females. The binding of uric acid to plasma proteins was estimated using micropartition system Amicon. The results obtained demonstrate a significant increase of uric acid binding during ovulatory and mid-luteal phase of menstrual cycle. No modifications are shown in post-menopausal females and in healthy males. No modifications have been shown with the same experiments performed in vitro. 相似文献
2.
F Petraglia F Facchinetti D Parrini A R Genazzani 《Bollettino della Società italiana di biologia sperimentale》1981,57(18):1862-1868
To evaluate the rabbit availability in beta-Lipotrophin studies we have investigated their brain, pituitary and plasmatic concentrations in basal conditions and after cold-stress. Previous silicic Acid extract and Sephadex G-75 column chromatography, the two peptides were measured through two specific and sensitive RIAs. The results reveal the presence of beta LPH and beta EP and their increase after cold-stress in hypothalamus, pituitary and peripheral plasma concentrations while not in midbrain. 相似文献
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Immature embryos culture in Italian red chicory 总被引:3,自引:0,他引:3
Varotto Serena Lucchin Margherita Parrini Paolo 《Plant Cell, Tissue and Organ Culture》2000,62(1):75-77
Chicory (Cichorium intybus L.) embryo maturation in vitro was achieved in B5 medium without growth regulators. Immature embryos were collected at different developmental stages 4–168
h after pollination. Only a few plantlets were obtained from ovules collected shortly after pollination since the embryos
at these early stages developed abnormal leaves and roots which regenerated shoots ‘via’ organogenesis. Zygotic embryos collected
24–72 h after pollination needed one month of culture in vitro before acclimation. Embryos collected at the heart or early torpedo stage of development (three days after pollination) produced
green plantlets after two weeks of in vitro culture and thus they could be transferred to the greenhouse for acclimation. Culture of zygotic embryos in vitro has shown to be a suitable technique to accelerate the breeding process in biennial cultivated Italian red chicory. In fact
it is possible to obtain plantlets ready for performing the selection for the desired agronomic traits one month after pollination.
Since flowering occurs after vernalization, the selection can be performed in the correct season before flowering.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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Determinants of Ras proteins specifying the sensitivity to yeast Ira2p and human p120-GAP. 总被引:1,自引:0,他引:1 下载免费PDF全文
Human and Saccharomyces cerevisiae Ras proteins and their regulators GAP (GTPase activating protein)and GEF (guanine nucleotide exchange factor) display structural similarities and are functionally interchangeable in vivo and in vitro, indicating that the molecular mechanism regulating Ras proteins has been conserved during evolution. As the only exceptions, the two S.cerevisiae GAPs, Ira1p and Ira2p, are strictly specific for yeast Ras proteins and cannot stimulate the GTPase of mammalian Ras. This study searches for the reasons for the different sensitivity to Ira2p of human H-ras p21 and yeast Ras2p. Construction of H-ras/Ras2p chimaeras showed that Gly18 of Ras2p (Ala11 of H-ras p21) is an important determinant for the specificity of Ira2p, revealing for the first time a function for this position. A second even more crucial determinant was found to be the 89-102 region of Ras2p (82-95 of H-ras p21) including the distal part of strand beta4, loop L6 and the proximal part of helix alpha3. It was possible to construct Ras2p's resistant to Ira2p but still sensitive to human p120-GAP and, conversely, a H-ras p21 sensitive to Ira2p. This work helps clarify specific aspects of the conserved molecular mechanism of interaction between Ras proteins and their negative GAP regulators. 相似文献
8.
Parrini C Taddei N Ramazzotti M Degl'Innocenti D Ramponi G Dobson CM Chiti F 《Structure (London, England : 1993)》2005,13(8):1143-1151
Six glycine residues of human muscle acylphosphatase (AcP) are evolutionarily conserved across the three domains of life. We have generated six variants of AcP, each having a glycine substituted by an alanine (G15A, G19A, G37A, G45A, G53A, and G69A). Three additional variants had Gly45 replaced by serine, glutamate, and arginine, respectively. The mutational variants do not, on average, have a lower conformational stability than other variants with substitutions of nonconserved residues. In addition, only the G15A variant is enzymatically inactive. However, all variants, with the exception of the G15A mutant, form amyloid aggregates more rapidly than the wild-type. Dynamic light-scattering experiments carried out under conditions close to physiological confirm that aggregate formation is generally more pronounced for the glycine-substituted variants. Apart from the glycine at position 15, all other conserved glycine residues in this protein could have been maintained during evolution because of their ability to inhibit aggregation. 相似文献
9.
An efficient and rapid procedure for plantlet regeneration from chicory mesophyll protoplasts 总被引:2,自引:0,他引:2
An efficient procedure for plantlet regeneration from chicory mesophyll protoplasts has been developed in order to perform
protoplast fusion experiments. Protoplasts were isolated from a genotype of Italian red chicory (CH 363) and purified by centrifugation
in a solution containing 13% (w/v) sucrose to collect uniform protoplasts in size. After 2 days culture at a density of 2×104 protoplasts ml−1 of liquid medium, protoplasts were cultured following three different procedures: in liquid medium, stratified in semi-solid
medium, and embedded in Ca-alginate droplets. Four different media were used and culture procedures were evaluated recording
the protoplast viability, protoplast division frequency and plating efficiency for each experiment. The embedding of protoplasts
in Ca-alginate droplets enhanced both division frequency and plating efficiency for chicory mesophyll cells. Furthermore,
this procedure shortened the cycle of plant regeneration from protoplasts, which could be completed in eight weeks.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
10.
Francesca Parrini Stefano Grignolio Siriano Luccarini Bruno Bassano Marco Apollonio 《Acta theriologica》2003,48(3):411-423
During a two year preliminary study, the spatial organization of a group of male Alpine ibexCapra ibex ibex Linnaeus, 1758 was examined in the Gran Paradiso National Park, Western Italian Alps, Italy. From December 1995 to January 1998 we measured annual, seasonal home range and home range during the rut, plus altitudinal migration of 13 radio-collared adult Alpine ibex. The small annual home range size showed a traditional use of space, confirmed by the high overlapping values between home ranges of consecutive years: the ibex used the same places from year to year. This was also true during periods of rut. Home ranges closely overlapped in consecutive ruts, while their size changed from winter to winter. Snow cover limited the movements of the ibex; winter and spring home ranges were smaller than those in summer and autumn. Mean vertical movement patterns were similar in the two years, showing the highest values in summer and the lowest in spring. Space use was never proportional to availability for each altitudinal range. 相似文献