全文获取类型
收费全文 | 129篇 |
免费 | 6篇 |
专业分类
135篇 |
出版年
2021年 | 1篇 |
2019年 | 1篇 |
2018年 | 3篇 |
2017年 | 2篇 |
2016年 | 2篇 |
2015年 | 5篇 |
2014年 | 2篇 |
2013年 | 3篇 |
2012年 | 6篇 |
2011年 | 6篇 |
2010年 | 6篇 |
2009年 | 4篇 |
2008年 | 12篇 |
2007年 | 8篇 |
2006年 | 8篇 |
2005年 | 9篇 |
2004年 | 12篇 |
2003年 | 8篇 |
2002年 | 17篇 |
2001年 | 4篇 |
2000年 | 2篇 |
1999年 | 4篇 |
1994年 | 1篇 |
1989年 | 2篇 |
1985年 | 2篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1975年 | 1篇 |
1974年 | 1篇 |
排序方式: 共有135条查询结果,搜索用时 15 毫秒
41.
This review reports and discusses the structural and the biological data available for the lipopolysaccharides from the Gram-negative bacterium Agrobacterium together with those of other related Rhizobiaceae species. 相似文献
42.
Brigitte Gasser Markku Saloheimo Ursula Rinas Martin Dragosits Escarlata Rodríguez-Carmona Kristin Baumann Maria Giuliani Ermenegilda Parrilli Paola Branduardi Christine Lang Danilo Porro Pau Ferrer Maria Luisa Tutino Diethard Mattanovich Antonio Villaverde 《Microbial cell factories》2008,7(1):1-18
Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. A major obstacle for protein production processes and a limit to overall success has been the abundance of misfolded polypeptides, which fail to reach their native conformation. The presence of misfolded or folding-reluctant protein species causes considerable stress in host cells. The characterization of such adverse conditions and the elicited cell responses have permitted to better understand the physiology and molecular biology of conformational stress. Therefore, microbial cell factories for recombinant protein production are depicted here as a source of knowledge that has considerably helped to picture the extremely rich landscape of in vivo protein folding, and the main cellular players of this complex process are described for the most important cell factories used for biotechnological purposes. 相似文献
43.
Francesca Salamanna Gianluca Giavaresi Deyanira Contartese Adriana Bigi Elisa Boanini Annapaola Parrilli Roberta Lolli Alessandro Gasbarrini Giovanni Barbanti Brodano Milena Fini 《Journal of cellular physiology》2019,234(11):20046-20056
Despite alternatives to autogenous bone graft for spinal fusion have been investigated, it has been shown that osteoconductive materials alone do not give a rate of fusion comparable with autogenous bone. This study analyzed a strontium substituted ß-tricalcium phosphate (Sr-ßTCP) associated with syngeneic, unexpanded, and undifferentiated mesenchymal stem cells from bone marrow (BMSC) or adipose tissue (ADSC) as a new tissue engineering approach for spinal fusion procedures. A posterolateral fusion was performed in 15 ovariectomized (OVX) and 15 sham-operated (SHAM) Inbred rats. Both SHAM and OVX animals were divided into three groups: Sr-ßTCP, Sr-ßTCP + BMCSs, and Sr-ßTCP + ADSCs. Animals were euthanized 8 weeks after surgery and the spines evaluated by manual palpation, micro-CT, and histology. For both SHAM and OVX animals, the fusion tissue in the Sr-ßTCP + BMSCs group was more solid. This effect was significantly higher in OVX animals by comparing the Sr-ßTCP + BMCSs group with Sr-ßTCP + ADSCs. Radiographical score, based on micro-CT 2D image, highlighted that the Sr-ßTCP + BMCSs group presented a similar fusion to Sr-ßTCP and higher than Sr-ßTCP + ADSCs in both SHAM and OVX animals. Micro-CT 3D parameters did not show significant differences among groups. Histological score showed significantly higher fusion in Sr-ßTCP + BMSCs group than Sr-ßTCP and Sr-ßTCP + ADSCs, for both SHAM and OVX animals. In conclusion, our results suggest that addition of BMSCs to a Sr-ßTCP improve bone formation and fusion, both in osteoporotic and nonosteoporotic animal, whereas spinal fusion is not enhanced in rats treated with Sr-ßTCP + ADSCs. Thus, for conducting cells therapy in spinal surgery BMSCs still seems to be a better choice compared with ADSCs. 相似文献
44.
Doherty GA Yang GX Borges E Tong S McCauley ED Treonz KM Van Riper G Pacholok S Si Q Koo GC Shah K Mumford RA Hagmann WK 《Bioorganic & medicinal chemistry letters》2003,13(11):1891-1895
A series of isonicotinoyl-(L)-aminophenylalanine derivatives was prepared and evaluated as VLA-4 antagonists. These compounds exhibit subnanomolar binding affinity to VLA-4 and significant off-rates. The interplay between off-rate, protein binding and pharmacokinetics is discussed. 相似文献
45.
Microbial degradation of aromatic hydrocarbons has been studied with the aim of developing applications for the removal of toxic compounds. Efforts have been directed toward the genetic manipulation of mesophilic bacteria to improve their ability to degrade pollutants, even though many pollution problems occur in sea waters and in effluents of industrial processes which are characterized by low temperatures. From these considerations the idea of engineering a psychrophilic microorganism for the oxidation of aromatic compounds was developed.In a previous paper it was demonstrated that the recombinant Antarctic Pseudoalteromonas haloplanktis TAC125 (PhTAC/tou) expressing a toluene-o-xylene monooxygenase (ToMO) is able to convert several aromatic compounds into corresponding catechols. In our work we improved the metabolic capability of PhTAC/tou cells by combining action of recombinant ToMO enzyme with that of the endogenous P. haloplanktis TAC125 laccase-like protein. This strategy allowed conferring new and specific degradative capabilities to a bacterium isolated from an unpolluted environment; indeed engineered PhTAC/tou cells are able to grow on aromatic compounds as sole carbon and energy sources. Our approach demonstrates the possibility to use the engineered psychrophilic bacterium for the bioremediation of chemically contaminated marine environments and/or cold effluents. 相似文献
46.
Isabel Maida Marco Fondi Maria Cristiana Papaleo Elena Perrin Valerio Orlandini Giovanni Emiliani Donatella de Pascale Ermenegilda Parrilli Maria Luisa Tutino Luigi Michaud Angelina Lo Giudice Riccardo Romoli Gianluca Bartolucci Renato Fani 《Extremophiles : life under extreme conditions》2014,18(1):35-49
Microorganisms from Antarctica have evolved particular strategies to cope with cold. Moreover, they have been recently reported as producers of antimicrobial compounds, which inhibit the growth of other bacteria. In this work we characterized from different viewpoints the Gillisia sp. CAL575 strain, a psychrotrophic bacterium that produces microbial volatile organic compounds involved in the growth inhibition of Burkholderia cepacia complex members. Sequencing and analysis of the whole genome of Gillisia sp. CAL575 revealed that it includes genes that are involved in secondary metabolite production, adaptation to cold conditions, and different metabolic pathways for the production of energy. All these features make Gillisia sp. CAL575 a possible tool for biotechnology. 相似文献
47.
Marco Fondi Valerio Orlandini Isabel Maida Elena Perrin Maria Cristiana Papaleo Giovanni Emiliani Donatella de Pascale Ermenegilda Parrilli Maria Luisa Tutino Luigi Michaud Angelina Lo Giudice Renato Fani 《Journal of bacteriology》2012,194(22):6334-6335
Arthrobacter sp. strain TB23 was isolated from the Antarctic sponge Lissodendoryx nobilis. This bacterium is able to produce antimicrobial compounds and volatile organic compounds (VOCs) that inhibit the growth of other Antarctic bacteria and of cystic fibrosis opportunistic pathogens, respectively. Here we report the draft genome sequence of Arthrobacter sp. TB23. 相似文献
48.
Silipo A Molinaro A Nazarenko EL Sturiale L Garozzo D Gorshkova RP Nedashkovskaya OI Lanzetta R Parrilli M 《Carbohydrate research》2005,340(16):2540-2549
49.
Cristina De Castro Olga De Castro Antonio Molinaro Michelangelo Parrilli 《European journal of biochemistry》2002,269(12):2885-2888
Agrobacterium tumefaciens is a Gram-negative, phytopathogenic bacterium and is characterized by an unique mode of action on dicotyledonous plants: it is able to genetically modify the host, and because of this feature, it is used as a tool for transgenic plants. Many experiments have demonstrated that lipopolysaccharides (LPSs) play an important role for the disease development, as they are involved in the adhesion process of the bacterium on the plant cell wall. Despite the wealth of information on the role of LPS on phytopathogenesis, the present paper appears as the first report on the molecular primary structure of the O-chain produced from Agrobacterium. Its repeating unit was determined by means of chemical and spectroscopical analysis, and has the following structure: (3)-alpha-D-Araf-(1-->3)-alpha-l-Fucp-(1-->. 相似文献
50.
Influence of growth temperature on lipid and phosphate contents of surface polysaccharides from the antarctic bacterium Pseudoalteromonas haloplanktis TAC 125 总被引:2,自引:0,他引:2 下载免费PDF全文
Corsaro MM Lanzetta R Parrilli E Parrilli M Tutino ML Ummarino S 《Journal of bacteriology》2004,186(1):29-34
The chemical structural variations induced by different growth temperatures in the lipooligosaccharide and exopolysaccharide components extracted from the Antarctic bacterium Pseudoalteromonas haloplanktis TAC 125 are described. The increase in phosphorylation with the increase in growth temperature seems to be general, because it happens not only for the lipooligosaccharide but also for the exopolysaccharide. Structural variations in the lipid components of lipid A also occur. In addition, free lipid A is found at both 25 and 4 degrees C but not at 15 degrees C, which is the optimal growth temperature, suggesting a incomplete biosynthesis of the lipooligosaccharide component under the first two temperature conditions. 相似文献