3D Printed Scaffolds Manufactured with Biosilica from Marine Sponges for Bone Healing in a Cranial Defect in Rats

Marine Biotechnology - The inorganic part of marine sponges, called Biosilica (BS), presents an osteogenic potential and the ability of consolidating fractures. Moreover, 3D printing technique is...     

The Mode of Action of Pederin,a Drug Inhibiting Protein Synthesis in Eucaryotic Organisms

Abstract

Pederin, a toxic substance isolated from the insect Paederus fuscipes, inhibits growth of Saccharomyces cerevisiae and EUE cells but not of Bacillus subtilis. Protein synthesis in vitro appears to be inhibited by the drug in preparations obtained from organisms containing 80 S ribosomes (yeast, EUE cells and rat liver) but not in those from organisms endowed with 70 S ribosomes (E. coli and B. subtilis). Pederin inhibits protein synthesis at a stage subsequent to the formation of the ternary complex between ribosomes, aminoacyl-tRNA and messenger RNA. Resistance or susceptibility to the drug appears to be a characteristic of ribosomes.     

Peptide Chain Initiation in Bacteria,Mitochondria and Chloroplasts

Abstract

Ribosomes prepared from the bacterium Bacillus subtilis, from mitochondria of Neurospora crassa and from chloroplasts of Euglena gracilis carry out the ApUpG-dependent reactions for peptide chain initiation demonstrated for Escherichia coli ribosomes. Initiation factors functionally similar to those from E. coli appear to be present on the above reported ribosomal preparations. The activity of ribosomes washed with high concentrations of ammonium chloride may be restored by adding initiation factors prepared from E. coli.